Fmoc-Asp(OtBu)-Wang Resin CAS129460-09-9 0.3~0.8 Mmol/g, 100~200 Mesh, 1% DVB

Fmoc-Asp(OtBu)-Wang Resin for C-Terminal Aspartic Acid Peptides

SHBC Fmoc-Asp(OtBu)-Wang Resin is a preloaded solid support for Fmoc solid-phase peptide synthesis of peptide acids containing C-terminal L-aspartic acid.

The resin contains N-Fmoc-L-aspartic acid beta-tert-butyl ester attached to an acid-labile Wang linker. The side-chain carboxyl group is protected by OtBu during peptide assembly.

After acidic cleavage and deprotection, the resin releases a peptide with a free C-terminal aspartic acid.

Product Specifications

Key Advantages of SHBC Fmoc-Asp(OtBu)-Wang Resin

Ready-to-Use Preloaded Support

The C-terminal Fmoc-Asp(OtBu) residue is already attached to the Wang linker, eliminating the initial amino acid loading step.

Produces C-Terminal Aspartic Acid Peptides

Acidic cleavage releases peptide acids ending in L-aspartic acid, with the side-chain carboxyl group deprotected.

Protected Aspartic Acid Side Chain

The OtBu group protects the beta-carboxyl group during repeated Fmoc deprotection and amino acid coupling cycles.

Flexible Loading Range

A loading range of 0.3–0.8 mmol/g supports different peptide lengths, sequence characteristics and production requirements.

Practical Resin Matrix

The 1% DVB-crosslinked polystyrene matrix and 100–200 mesh particle size provide a useful balance of swelling, reagent accessibility, filtration and washing.

Suitable for Research and Batch Synthesis

The resin can support laboratory synthesis, process development and qualified batch peptide production.

How Fmoc-Asp(OtBu)-Wang Resin Works

Fmoc-Asp(OtBu) is attached to the Wang linker through the amino acid alpha-carboxyl group. Its beta-carboxyl group remains protected as an OtBu ester during peptide assembly.

A general synthesis workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Remove the Fmoc protecting group.

3. Couple the next protected amino acid.

4. Repeat Fmoc deprotection and coupling cycles.

5. Wash the resin between each reaction step.

6. Cleave the completed peptide under validated acidic conditions.

7. Purify and characterize the resulting peptide acid.

Acidic cleavage releases the peptide from the Wang linker and removes the OtBu side-chain protection.

Applications

SHBC Fmoc-Asp(OtBu)-Wang Resin can be used for:

• Fmoc solid-phase peptide synthesis

• Peptides with C-terminal aspartic acid

• C-terminal peptide acid preparation

• Therapeutic peptide research

• Enzyme substrate peptide synthesis

• Antigenic and epitope peptides

• Peptide reference standards

• Biochemical research peptides

• Modified and labeled peptides

• Peptide library synthesis

• Process development and batch production

Choosing the Right Resin Loading

Resin loading affects synthesis capacity, reagent diffusion and peptide aggregation.

Lower loading may be preferred for:

• Long peptide sequences

• Hydrophobic peptides

• Sterically demanding amino acids

• Aggregation-prone sequences

Higher loading may provide greater productivity for shorter and less demanding peptides.

Select the loading according to peptide length, sequence hydrophobicity, synthesis scale and purification requirements.

Product Identification and CAS Clarification

Fmoc-Asp(OtBu)-Wang Resin is a polymer-bound product and should be identified using its complete product name, loading, particle size, DVB crosslinking and lot-specific documentation.

CAS 129460-09-9 identifies free Fmoc-Asp-OtBu, an alpha-tert-butyl ester compound. It is not a unique CAS number for the preloaded Wang resin.

For purchasing and regulatory documentation, refer to the SHBC product label, COA and SDS.

Quality Control and Storage

Important qualification parameters include:

• Fmoc loading or substitution

• Amino acid identity

• Particle-size distribution

• DVB crosslinking

• Enantiomeric purity

• Dipeptide content

• Moisture content

• Resin swelling performance

• Residual unreacted linker sites

• Test coupling and cleavage performance

• Lot-to-lot consistency

Request the lot-specific SHBC COA for available test results.

Store the resin tightly sealed in a cool, dry place according to the product label and SDS. Pre-swell the resin completely before use.

Frequently Asked Questions

What is Fmoc-Asp(OtBu)-Wang Resin used for?

It is used in Fmoc SPPS to prepare peptide acids containing C-terminal L-aspartic acid.

What peptide C-terminus is obtained after cleavage?

Validated acidic cleavage releases a peptide with a free C-terminal L-Asp carboxylic acid.

Why is the aspartic acid side chain protected with OtBu?

The OtBu group protects the beta-carboxyl group during peptide-chain assembly and is removed during acidic cleavage.

Is the first amino acid already loaded?

Yes. Fmoc-Asp(OtBu) is preloaded onto the Wang linker, allowing synthesis to begin with Fmoc removal and coupling of the next amino acid.

Is CAS 129460-09-9 the CAS number of this resin?

No. CAS 129460-09-9 identifies free Fmoc-Asp-OtBu, not the polymer-bound Fmoc-Asp(OtBu)-Wang Resin. Use the SHBC COA and SDS for product identification.

What loading should I choose?

Lower loading is often selected for long or aggregation-prone peptides. Higher loading may improve production capacity for shorter sequences.

What information is required for a quotation?

Please provide the required quantity, target loading, peptide length, synthesis scale, packaging requirements and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies Fmoc-Asp(OtBu)-Wang Resin for Fmoc peptide synthesis, process development and batch production.

Contact Shanghai SanYu Biotechnology Co., Ltd. to request evaluation samples, bulk pricing, loading options, packaging information, COA and SDS.

For research and process-development use unless otherwise specified.