Fmoc-His(Trt)-Wang Resin CAS109425-51-6 0.3~0.8 Mmol/g, 100~200 Mesh, 1% DVB

Fmoc-His(Trt)-Wang Resin for C-Terminal Histidine Peptides

SHBC Fmoc-His(Trt)-Wang Resin is a preloaded solid support for the Fmoc solid-phase peptide synthesis of peptide acids containing C-terminal L-histidine.

The resin contains N-alpha-Fmoc-Nim-trityl-L-histidine attached to an acid-labile Wang linker on a 1% DVB-crosslinked polystyrene matrix.

The Fmoc group protects the alpha-amino group, while the Trt group protects the histidine imidazole side chain during peptide assembly. Acidic cleavage releases the peptide as C-terminal L-His-OH and removes the Trt protecting group.

Product Specifications

Key Advantages of SHBC Fmoc-His(Trt)-Wang Resin

Ready-to-Use Preloaded Support

The C-terminal histidine derivative is already attached to the Wang linker, eliminating the separate amino acid loading step.

Produces C-Terminal Histidine Peptide Acids

Cleavage releases a peptide with a C-terminal L-histidine carboxylic acid, making the resin suitable for sequences ending in His-OH.

Trt-Protected Imidazole Side Chain

The trityl group protects the histidine imidazole nitrogen during repeated Fmoc deprotection and coupling cycles.

Compatible with Fmoc SPPS

The resin is suitable for standard manual and automated Fmoc peptide synthesis workflows.

Flexible Loading Range

A loading range of 0.3–0.8 mmol/g allows users to select an appropriate balance between synthesis capacity and reagent accessibility.

Practical Resin Handling

The 100–200 mesh, 1% DVB polystyrene matrix supports effective swelling, washing, filtration and reagent diffusion in compatible SPPS solvents.

How Fmoc-His(Trt)-Wang Resin Works

Fmoc-His(Trt)-OH is attached to the Wang linker through an acid-labile ester bond. The preloaded histidine becomes the C-terminal residue of the target peptide.

A typical synthesis workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Remove the Fmoc protecting group.

3. Couple the next protected amino acid.

4. Repeat Fmoc deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave and deprotect the completed peptide under validated acidic conditions.

7. Purify and characterize the resulting peptide acid.

Coupling and cleavage conditions should be optimized according to the peptide sequence, resin loading and synthesis scale.

Applications

SHBC Fmoc-His(Trt)-Wang Resin can be used for:

• C-terminal histidine peptide synthesis

• Fmoc solid-phase peptide synthesis

• Peptide acid preparation

• Histidine-containing peptide libraries

• Enzyme substrate peptide synthesis

• Antigenic and epitope peptide production

• Metal-binding and coordination peptide research

• Peptide reference standard preparation

• Modified and labeled peptide synthesis

• Therapeutic peptide research

• Process development and batch peptide production

It is particularly suitable when L-histidine is required as the final C-terminal residue.

Choosing the Right Resin Loading

Resin loading affects theoretical production capacity, coupling accessibility and peptide aggregation.

Lower loading may be preferred for:

• Long peptide sequences

• Hydrophobic peptides

• Aggregation-prone sequences

• Sterically demanding amino acids

• Difficult coupling processes

Higher loading may provide greater batch productivity for shorter or less demanding sequences.

Pilot-scale evaluation is recommended before transferring a synthesis process to larger batches.

Comparison with Other SPPS Supports

Choose Fmoc-His(Trt)-Wang Resin when the target product requires a C-terminal L-histidine carboxylic acid.

Product Identification and CAS Clarification

CAS 109425-51-6 identifies the free amino acid derivative Fmoc-His(Trt)-OH. It is not a unique CAS number for the polymer-bound Fmoc-His(Trt)-Wang Resin.

The resin product should be identified using:

• Product name

• SHBC product or catalog number

• Loading range

• Particle size

• DVB crosslinking

• Lot number

• SHBC Certificate of Analysis

• SHBC Safety Data Sheet

Contact SHBC if CAS-specific documentation is required for purchasing or customs purposes.

Quality Control and Storage

Recommended qualification parameters include:

• Fmoc amino acid loading

• Resin identity

• Particle-size distribution

• DVB crosslinking

• Moisture content

• Swelling performance

• Stereochemical purity

• D-histidine content

• Dipeptide content

• Trt protecting-group integrity

• Test coupling and cleavage performance

• Lot-to-lot consistency

Keep the resin tightly sealed in a cool, dry place according to the product label and SDS. Protect it from moisture, contamination and prolonged exposure to unsuitable conditions.

Pre-swell the resin completely before use.

Frequently Asked Questions

What is Fmoc-His(Trt)-Wang Resin used for?

It is used in Fmoc SPPS to prepare peptide acids containing C-terminal L-histidine.

Is the histidine already loaded onto the resin?

Yes. Fmoc-His(Trt)-OH is preloaded onto the Wang linker, reducing the number of preparation steps required before peptide assembly.

What does the Trt group protect?

The Trt group protects the imidazole side chain of histidine during Fmoc deprotection and amino acid coupling cycles.

Is the Trt group removed during cleavage?

It is normally removed together with peptide cleavage and side-chain deprotection under validated acidic conditions.

What C-terminus is obtained after cleavage?

The resulting peptide normally contains a C-terminal L-histidine carboxylic acid, written as His-OH.

Is this resin suitable for peptide amide synthesis?

No. Standard Fmoc-His(Trt)-Wang Resin produces a peptide acid. A suitable Rink Amide-based support should be selected for C-terminal peptide amides.

Is CAS 109425-51-6 the CAS number of the resin?

The CAS number identifies free Fmoc-His(Trt)-OH. Refer to the SHBC COA, SDS and product number for identification of the preloaded resin.

Which loading should I select?

Lower loading may benefit long or aggregation-prone peptides. Higher loading may increase productivity for shorter and less demanding sequences.

What information is required for a quotation?

Please provide the required quantity, target loading, peptide application, packaging requirements and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies Fmoc-His(Trt)-Wang Resin for research, process development and batch peptide synthesis.

Contact Shanghai SanYu Biotechnology Co., Ltd. to discuss evaluation samples, loading requirements, bulk packaging, COA, SDS and quotation details.

For research and process-development use only unless otherwise specified.