Fmoc-Ile-Wang Resin CAS71989-23-6 0.3~0.8mmol/g, 100~200 Mesh, 1% DVB

Fmoc-Ile-Wang Resin for C-Terminal Isoleucine Peptides

SHBC Fmoc-Ile-Wang Resin is a preloaded solid support for Fmoc solid-phase peptide synthesis of peptide acids containing C-terminal L-isoleucine.

The resin contains N-Fmoc-L-isoleucine attached to an acid-labile Wang linker on a 1% DVB-crosslinked polystyrene matrix. Its 100–200 mesh particle size supports effective swelling, washing, filtration and reagent contact.

After peptide assembly, acidic cleavage releases the target peptide with a C-terminal L-isoleucine carboxylic acid.

Product Specifications

Key Advantages of SHBC Fmoc-Ile-Wang Resin

Ready-to-Use Preloaded Resin

Fmoc-L-isoleucine is already attached to the Wang linker, eliminating the separate initial amino acid loading step.

Produces C-Terminal Isoleucine Peptide Acids

Acidic cleavage releases a peptide containing C-terminal L-Ile-OH, without requiring an additional solution-phase modification step.

Compatible with Fmoc SPPS

The resin supports standard Fmoc deprotection and amino acid coupling cycles used in manual and automated peptide synthesis.

No Isoleucine Side-Chain Protection Required

The non-reactive aliphatic side chain of isoleucine normally requires no additional protecting group during peptide assembly.

Flexible Loading Range

The 0.3–0.8 mmol/g loading range allows users to balance theoretical synthesis capacity with reagent accessibility and aggregation control.

Practical Resin Handling

The 100–200 mesh, 1% DVB polystyrene matrix provides a useful balance between swelling, reagent diffusion, mechanical stability and filtration speed.

How Fmoc-Ile-Wang Resin Works

Fmoc-L-isoleucine is attached to the Wang linker through an acid-labile ester bond. The preloaded isoleucine becomes the C-terminal residue of the target peptide.

A typical synthesis workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Remove the Fmoc protecting group.

3. Couple the next protected amino acid.

4. Repeat Fmoc deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave and deprotect the completed peptide under validated acidic conditions.

7. Purify and characterize the resulting peptide acid.

Coupling, deprotection and cleavage conditions should be optimized for the peptide sequence, loading and synthesis scale.

Applications

SHBC Fmoc-Ile-Wang Resin can be used for:

• C-terminal isoleucine peptide synthesis

• Fmoc solid-phase peptide synthesis

• Peptide acid preparation

• Hydrophobic peptide research

• Enzyme substrate and inhibitor peptides

• Antigenic and epitope peptides

• Peptide structure–activity studies

• Peptide library synthesis

• Modified and labeled peptides

• Peptide reference standards

• Therapeutic peptide research

• Process development and batch peptide production

The resin is particularly useful when L-isoleucine is required as the final C-terminal amino acid.

Choosing the Right Resin Loading

Resin loading affects theoretical production capacity, coupling efficiency and peptide aggregation.

Lower loading may be preferred for:

• Long peptide sequences

• Hydrophobic peptides

• Aggregation-prone sequences

• Sterically demanding couplings

• Difficult or modified peptides

Higher loading may increase batch productivity for shorter and less demanding sequences.

Pilot-scale synthesis is recommended before transferring a process to larger batches.

Comparison with Other SPPS Supports

Choose Fmoc-Ile-Wang Resin when the target peptide requires a C-terminal L-isoleucine carboxylic acid.

Product Identification and CAS Clarification

CAS 71989-23-6 identifies the free amino acid derivative Fmoc-Ile-OH. It is not a unique CAS number for polymer-bound Fmoc-Ile-Wang Resin.

The preloaded resin should be identified using:

• Product name

• SHBC product or catalog number

• Loading range

• Particle size

• DVB crosslinking

• Lot number

• Certificate of Analysis

• Safety Data Sheet

Contact SHBC if product-specific identification documents are required for purchasing, customs or technical evaluation.

Quality Control and Storage

Recommended qualification parameters include:

• Fmoc amino acid loading

• Resin identity

• Particle-size distribution

• DVB crosslinking

• Moisture content

• DMF swelling performance

• Enantiomeric purity

• D-allo-isoleucine content

• Dipeptide content

• Test coupling performance

• Test cleavage performance

• Lot-to-lot consistency

Isoleucine contains two stereogenic centers. Control of epimerization during resin loading is important for maintaining the required stereochemical purity.

Keep the resin tightly sealed in a cool, dry place according to the product label and SDS. Protect it from moisture, contamination and prolonged exposure to unsuitable conditions.

Pre-swell the resin completely before use.

Frequently Asked Questions

What is Fmoc-Ile-Wang Resin used for?

It is used in Fmoc SPPS to synthesize peptide acids containing C-terminal L-isoleucine.

Is the first amino acid already loaded?

Yes. Fmoc-L-isoleucine is preloaded onto the Wang linker, reducing resin preparation steps before peptide assembly.

What C-terminus is obtained after cleavage?

The resulting peptide normally contains a C-terminal L-isoleucine carboxylic acid, written as Ile-OH.

Does isoleucine require side-chain protection?

No. Its aliphatic side chain normally does not require an additional protecting group during Fmoc SPPS.

Is this resin suitable for peptide amide synthesis?

No. Standard Fmoc-Ile-Wang Resin produces a peptide acid. A suitable Rink Amide support should be selected for C-terminal peptide amides.

What is the difference between Fmoc-Ile-Wang Resin and Fmoc-Ile-OH?

Fmoc-Ile-Wang Resin is a polymer-bound, preloaded synthesis support. Fmoc-Ile-OH is a soluble protected amino acid building block.

Is CAS 71989-23-6 the CAS number of the resin?

The CAS number identifies free Fmoc-Ile-OH. Refer to the SHBC product number, COA and SDS for identification of the preloaded resin.

Which loading should I select?

Lower loading may benefit long, hydrophobic or aggregation-prone peptides. Higher loading may provide greater capacity for shorter sequences.

What information is required for a quotation?

Please provide the required quantity, target loading, peptide application, packaging requirements and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies Fmoc-Ile-Wang Resin for research, process development and batch peptide synthesis.

Contact Shanghai SanYu Biotechnology Co., Ltd. to discuss evaluation samples, loading requirements, bulk packaging, COA, SDS and quotation details.

For research and process-development use only unless otherwise specified.