Fmoc-Leu-Wang Resin CAS35661-60-0 0.3~0.8mmol/g, 100~200 Mesh, 1% DVB

Fmoc-Leu-Wang Resin for C-Terminal Leucine Peptides

SHBC Fmoc-Leu-Wang Resin is a preloaded solid support for Fmoc solid-phase peptide synthesis of peptide acids containing C-terminal L-leucine.

The resin contains N-Fmoc-L-leucine attached to an acid-labile Wang linker on a 1% DVB-crosslinked polystyrene matrix. Its 100–200 mesh particle size supports effective swelling, reagent diffusion, washing and filtration.

Acidic cleavage releases the completed peptide with a C-terminal L-leucine carboxylic acid.

Product Specifications

Key Advantages of SHBC Fmoc-Leu-Wang Resin

Ready-to-Use Preloaded Support

Fmoc-L-leucine is already attached to the Wang linker, eliminating the separate first amino acid loading step.

Produces C-Terminal Leucine Peptide Acids

Cleavage releases a peptide containing C-terminal L-Leu-OH without requiring additional solution-phase modification.

Compatible with Fmoc SPPS

The resin supports standard Fmoc deprotection and amino acid coupling cycles used in manual and automated peptide synthesis.

No Leucine Side-Chain Protection Required

The non-reactive aliphatic side chain of leucine normally requires no additional protecting group during peptide-chain assembly.

Flexible Loading Range

The 0.3–0.8 mmol/g range allows users to balance synthesis capacity, reagent accessibility and aggregation control.

Practical Resin Handling

The 100–200 mesh, 1% DVB polystyrene matrix provides a useful balance between swelling, mechanical stability, filtration speed and reagent diffusion.

How Fmoc-Leu-Wang Resin Works

Fmoc-L-leucine is attached to the Wang linker through an acid-labile ester bond. The preloaded leucine becomes the C-terminal residue of the target peptide.

A typical synthesis workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Remove the Fmoc protecting group.

3. Couple the next protected amino acid.

4. Repeat Fmoc deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave and deprotect the completed peptide under validated acidic conditions.

7. Purify and characterize the resulting peptide acid.

Reaction conditions should be optimized according to the peptide sequence, resin loading and synthesis scale.

Applications

SHBC Fmoc-Leu-Wang Resin can be used for:

• C-terminal leucine peptide synthesis

• Fmoc solid-phase peptide synthesis

• Peptide acid preparation

• Hydrophobic and amphipathic peptide research

• Enzyme substrate and inhibitor peptides

• Antigenic and epitope peptides

• Protein fragment synthesis

• Peptide structure–activity studies

• Peptide library preparation

• Modified and labeled peptides

• Peptide reference standards

• Therapeutic peptide research

• Process development and batch peptide production

The resin is particularly suitable when L-leucine is required as the final C-terminal amino acid.

Choosing the Right Resin Loading

Resin loading affects theoretical production capacity, coupling accessibility and peptide aggregation.

Lower loading may be preferred for:

• Long peptide sequences

• Hydrophobic peptides

• Aggregation-prone sequences

• Sterically demanding couplings

• Difficult or highly modified peptides

Higher loading may provide greater batch productivity for shorter and less demanding sequences.

Pilot-scale synthesis is recommended before transferring the process to larger batches.

Comparison with Other SPPS Supports

Choose Fmoc-Leu-Wang Resin when the target product requires a C-terminal L-leucine carboxylic acid.

Product Identification and CAS Clarification

CAS 35661-60-0 identifies the free amino acid derivative Fmoc-Leu-OH. It is not a unique CAS number for polymer-bound Fmoc-Leu-Wang Resin.

The preloaded resin should be identified using:

• Product name

• SHBC product or catalog number

• Loading range

• Particle size

• DVB crosslinking

• Lot number

• Certificate of Analysis

• Safety Data Sheet

Contact SHBC if product-specific identification documents are required for purchasing, customs or technical evaluation.

Quality Control and Storage

Recommended qualification parameters include:

• Fmoc amino acid loading

• Resin identity

• Particle-size distribution

• DVB crosslinking

• Moisture content

• DMF swelling performance

• Enantiomeric purity

• D-leucine content

• Dipeptide content

• Test coupling performance

• Test cleavage performance

• Lot-to-lot consistency

Control of epimerization and dipeptide formation during resin loading is important for reliable peptide synthesis.

Keep the resin tightly sealed in a cool, dry place according to the product label and SDS. Protect it from moisture, contamination and prolonged exposure to unsuitable conditions.

Pre-swell the resin completely before use.

Frequently Asked Questions

What is Fmoc-Leu-Wang Resin used for?

It is used in Fmoc SPPS to synthesize peptide acids containing C-terminal L-leucine.

Is the first amino acid already loaded?

Yes. Fmoc-L-leucine is preloaded onto the Wang linker, reducing resin preparation steps before peptide synthesis.

What C-terminus is obtained after cleavage?

The resulting peptide normally contains a C-terminal L-leucine carboxylic acid, written as Leu-OH.

Does leucine require side-chain protection?

No. Its aliphatic side chain normally does not require an additional protecting group during Fmoc SPPS.

Is this resin suitable for peptide amide synthesis?

No. Fmoc-Leu-Wang Resin produces a peptide acid. A suitable Rink Amide support should be selected for C-terminal peptide amides.

What is the difference between Fmoc-Leu-Wang Resin and Fmoc-Leu-OH?

Fmoc-Leu-Wang Resin is a polymer-bound, preloaded synthesis support. Fmoc-Leu-OH is a soluble protected amino acid building block.

Is CAS 35661-60-0 the CAS number of the resin?

The CAS number identifies free Fmoc-Leu-OH. Refer to the SHBC product number, COA and SDS for identification of the preloaded resin.

Which loading should I select?

Lower loading may benefit long, hydrophobic or aggregation-prone peptides. Higher loading may provide greater capacity for shorter sequences.

What information is required for a quotation?

Please provide the required quantity, target loading, peptide application, packaging requirements and required quality documents.

Request a Sample or Bulk Quote

SHBC supplies Fmoc-Leu-Wang Resin for research, process development and batch peptide synthesis.

Contact Shanghai SanYu Biotechnology Co., Ltd. to discuss evaluation samples, loading requirements, bulk packaging, COA, SDS and quotation details.

For research and process-development use only unless otherwise specified.