Product Overview
SHBC IMAC TopAC HP is an NTA-based metal chelate affinity chromatography resin designed for protein separation and purification.
The TopAC-IMAC FF/HP series consists of cross-linked agarose and nitrilotriacetic acid (NTA) and can chelate metal ions including Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺ and Fe³⁺.
IMAC TopAC HP has a specified Ni²⁺ chelating metal capacity of approximately 15 μmol/mL.
Chelated metal ions can interact with histidine, cysteine and tryptophan residues in proteins, enabling affinity-based protein separation and purification.
Key Features
NTA-based metal chelate chromatography resin
Cross-linked agarose matrix
≈15 μmol/mL Ni²⁺ chelating capacity
Supports multiple metal ions
Good physical stability
Good chemical stability
High specificity
Suitable for protein separation
Suitable for protein purification
Package sizes from 25 mL to 20 L
Technical Specifications
Parameter | Specification |
|---|
Product Name | IMAC TopAC HP |
Brand | SHBC |
English Name | Ni-NTA Affinity Chromatography Resin HP |
Series | HP |
Matrix | Cross-linked Agarose |
Ligand | NTA (Nitrilotriacetic Acid) |
Reference Metal Ion | Ni²⁺ |
Chelating Metal Capacity | ≈15 μmol/mL (Ni²⁺) |
Chelatable Metal Ions | Cu²⁺ / Zn²⁺ / Ni²⁺ / Co²⁺ / Fe³⁺ |
Chromatography Mode | Metal Chelate Affinity Chromatography |
Main Application | Protein Separation and Purification |
Package | 25 mL / 100 mL / 300 mL / 1 L / 5 L / 10 L / 20 L |
SHBC Art.No. | Packaging Specification |
|---|
A5036 | 25 mL |
A5037 | 100 mL |
A5038 | 300 mL |
A5039 | 1 L |
A5040 | 5 L |
A5041 | 10 L |
A5042 | 20 L |
IMAC TopAC HP uses NTA (nitrilotriacetic acid) as the metal-chelating ligand.
NTA is combined with the cross-linked agarose matrix to provide sites capable of coordinating metal ions for immobilized metal affinity chromatography.
The resulting metal-chelate structure provides affinity interactions that can be used for protein separation.
The specified chelating metal capacity of IMAC TopAC HP is:
≈15 μmol/mL (Ni²⁺)
This specification represents the resin's Ni²⁺ metal chelating capacity and should not be interpreted as protein binding capacity.
According to the supplied TopAC-IMAC FF/HP series information, the resin can chelate:
The NTA structure helps improve the stability of the chelated metal ions.
Protein Interaction Mechanism
The chelated metal ions can interact with amino acid residues in proteins, including:
Histidine
Cysteine
Tryptophan
These interactions provide the affinity mechanism used to separate and purify suitable proteins.
Cross-Linked Agarose Matrix
IMAC TopAC HP is based on a cross-linked agarose matrix.
According to the supplied series description, the chromatography medium provides good physical and chemical stability, supporting its use in protein purification workflows.
High Specificity
The supplied product information identifies high specificity as one of the advantages of the TopAC-IMAC FF/HP series.
Metal ion-mediated interactions with suitable amino acid residues enable selective affinity separation of proteins under appropriate chromatographic conditions.
Protein Separation and Purification
IMAC TopAC HP can be used for affinity-based protein separation and purification.
The NTA ligand first coordinates suitable metal ions. These immobilized metal ions subsequently interact with specific residues on proteins, enabling chromatographic separation.
Applications
SHBC IMAC TopAC HP can be used for:
Immobilized metal affinity chromatography
Metal chelate affinity chromatography
Protein separation
Protein purification
Affinity chromatography
Biomolecule purification
Protein downstream processing
Chromatography method development
Product Advantages
IMAC TopAC HP provides:
NTA chelating ligand
Cross-linked agarose matrix
≈15 μmol/mL Ni²⁺ chelating capacity
Multiple metal ion compatibility
Good physical stability
Good chemical stability
High specificity
Suitable for protein separation and purification
Package sizes up to 20 L
IMAC TopAC HP vs. IMAC TopAC FF
Both products belong to the supplied TopAC-IMAC FF/HP series.
Feature | IMAC TopAC HP | IMAC TopAC FF |
|---|
English Name | Ni-NTA Affinity Chromatography Resin HP | Ni-NTA Affinity Chromatography Resin FF |
Series | HP | FF |
Matrix | Cross-linked Agarose | Cross-linked Agarose |
Ligand | NTA | NTA |
Chelating Capacity | ≈15 μmol/mL Ni²⁺ | ≈15 μmol/mL Ni²⁺ |
Metal Ions | Cu²⁺ / Zn²⁺ / Ni²⁺ / Co²⁺ / Fe³⁺ | Cu²⁺ / Zn²⁺ / Ni²⁺ / Co²⁺ / Fe³⁺ |
Package | 25 mL–20 L | 25 mL–20 L |
The supplied table does not provide separate particle size, pressure resistance or quantitative flow-rate specifications for the HP and FF versions, so these parameters are not added.
Available Package Sizes
IMAC TopAC HP is available in:
25 mL
100 mL
300 mL
1 L
5 L
10 L
20 L
Frequently Asked Questions
What is IMAC TopAC HP?
IMAC TopAC HP is an NTA-based metal chelate affinity chromatography resin designed for protein separation and purification.
What is the English product name?
The supplied English name is Ni-NTA Affinity Chromatography Resin HP.
What matrix is used?
The TopAC-IMAC FF/HP series uses cross-linked agarose.
What ligand is used?
The resin uses NTA (nitrilotriacetic acid) as the chelating ligand.
What is the Ni²⁺ chelating capacity?
The specified capacity is approximately 15 μmol/mL (Ni²⁺).
Is 15 μmol/mL the protein binding capacity?
No. It is the Ni²⁺ chelating metal capacity, not protein binding capacity.
The supplied series information lists Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺ and Fe³⁺.
The supplied information lists histidine, cysteine and tryptophan residues.
What is IMAC TopAC HP used for?
It is used for metal chelate affinity chromatography and protein separation and purification.
Is the particle size specified?
No. The supplied table does not provide a particle-size specification.
Is the pressure resistance specified?
No. A quantitative pressure-resistance value is not provided in the supplied table.
What package sizes are available?
Available packages are 25 mL, 100 mL, 300 mL, 1 L, 5 L, 10 L and 20 L.
What are the SHBC Art.No. numbers?
The corresponding Art.No. are A5036, A5037, A5038, A5039, A5040, A5041 and A5042.
What brand is IMAC TopAC HP?
IMAC TopAC HP is supplied under the SHBC brand.
