1.5um NHS Magnetic Beads MNHS1.5UM-10 5%

1.5µm NHS Magnetic Beads for Immunoassay Research

SHBC MNHS1.5UM-10 NHS Magnetic Beads are 1.5µm magnetic particles with an NHS-activated surface.

They are designed for direct coupling of antibodies, antigens, proteins, peptides and other amine-containing biomolecules.

The product is supplied at 5% solids for immunoassay research, magnetic separation, affinity capture and bulk reagent production.

Product: 1.5µm NHS Magnetic Beads
Catalog Number: MNHS1.5UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 1.5µm
Surface: NHS activated
Solids Content: 5%
Main Application: Immunoassay research
Supply: Samples, pilot batches and bulk production
Intended Use: Research use only

Product Overview

MNHS1.5UM-10 provides a ready-to-couple magnetic surface.

The NHS groups react with primary amino groups on antibodies and proteins.

This forms stable covalent amide bonds.

No additional EDC/NHS activation is normally required before protein coupling.

The 1.5µm particle size provides a practical balance between:

• Particle number

• Collective surface area

• Magnetic response

• Suspension stability

• Bead recovery

• Automated washing

The magnetic format simplifies separation of bound and unbound reagents.

Technical Specifications

The following data should be confirmed by the final TDS or lot-specific COA:

• Actual particle-size range

• Particle-size CV

• Particle morphology

• Magnetic material

• Magnetic content

• Magnetic response time

• NHS-group density

• Antibody coupling capacity

• Protein coupling capacity

• Particle concentration

• Suspension buffer

• Preservative

• Package size

• Shelf life

• Storage temperature

The 5% solids content alone cannot determine coupling capacity or magnetic response time.

Key Advantages

Ready-to-Couple NHS Surface

The bead surface is already NHS activated.

The user does not need to perform a separate EDC/NHS activation step.

This reduces:

• Reagent preparation

• Activation time

• Intermediate washing

• Process variation

• Operator workload

Direct Antibody Coupling

Antibodies containing accessible primary amino groups can react directly with the NHS surface.

The process is suitable for monoclonal and polyclonal antibodies.

Stable Covalent Immobilization

NHS esters react with primary amines to form stable amide bonds.

Covalent attachment helps reduce ligand loss during washing, incubation and storage.

Balanced 1.5µm Particle Size

The 1.5µm size combines a high particle number with practical magnetic collection.

It is suitable for small-volume reactions and automated immunoassay development.

Magnetic Separation

The beads can be collected with an external magnetic field.

This supports:

• Fast washing

• Buffer exchange

• Removal of unbound reagents

• Target enrichment

• Automated assay processing

5% Solids Content

The concentrated suspension supports:

• Laboratory evaluation

• Process optimization

• Pilot production

• Bulk reagent preparation

Automation Potential

MNHS1.5UM-10 can be evaluated in manual, semi-automated and automated magnetic immunoassay workflows.

Magnetic response and resuspension must be validated on the intended analyzer.

Bulk Manufacturing

SHBC supports:

• Research samples

• Pilot batches

• Repeat orders

• OEM supply

• Private-label packaging

• Enterprise bulk production

How NHS Coupling Works

NHS esters mainly react with primary amino groups.

Primary amino groups are found on:

• Lysine residues

• Protein N-termini

• Peptide N-termini

• Amino-modified oligonucleotides

• Amino-modified synthetic ligands

The simplified reaction is:

NHS Magnetic Bead + Amine-Containing Ligand → Covalently Coupled Bead–Ligand

The reaction forms a stable amide linkage.

Use an Amine-Free Buffer

The coupling buffer should not contain competing primary amines.

Avoid during coupling:

• Tris

• Glycine

• Ethanolamine

• Amine-containing stabilizers

• High levels of free amino acids

These substances can consume NHS groups and reduce ligand loading.

Control NHS Hydrolysis

NHS groups are moisture sensitive and can hydrolyze in aqueous solution.

Prepare the beads immediately before coupling.

Do not leave activated beads in coupling buffer longer than necessary.

Quench Remaining NHS Groups

After ligand coupling, remaining active NHS groups should be quenched.

This helps reduce:

• Unwanted surface reactions

• Non-specific protein binding

• Assay background

• Lot-to-lot variation

Immunoassay Applications

Magnetic Sandwich Immunoassays

Couple a capture antibody to MNHS1.5UM-10.

The antibody-coated beads capture the target antigen.

A labeled detection antibody then generates the signal.

Potential research targets include:

• Hormones

• Cytokines

• Tumor biomarkers

• Cardiac biomarkers

• Inflammatory markers

• Infectious-disease antigens

• Autoantibodies

• Veterinary biomarkers

Chemiluminescent Immunoassays

MNHS1.5UM-10 can be evaluated as a magnetic solid phase in CLIA and CLEIA development.

The magnetic beads support:

• Target capture

• Magnetic washing

• Reporter binding

• Substrate reaction

• Luminescence measurement

Fluorescent Immunoassays

Fluorescent detection antibodies or probes may be combined with the magnetic solid phase.

Enzyme Immunoassays

The beads can be used with enzyme-labeled detection reagents and compatible substrates.

Competitive Immunoassays

Potential targets include:

• Small molecules

• Drugs

• Haptens

• Hormones

• Toxins

• Metabolites

• Peptides

Antigen-Coated Magnetic Beads

Recombinant antigens and proteins may be coupled for:

• Antibody detection

• Serological research

• Autoantibody studies

• Vaccine-response research

• Antibody screening

Affinity Capture

Antibody- or protein-coated beads may be used for:

• Target enrichment

• Immunoprecipitation research

• Protein purification research

• Antigen capture

• Protein interaction studies

Suitable Coupling Molecules

MNHS1.5UM-10 may be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant antibodies

• Antibody fragments

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Lectins

• Receptors

• Amino-modified oligonucleotides

• Amino-modified aptamers

• Amine-containing synthetic ligands

The molecule must contain an accessible primary amino group.

Recommended Coupling Workflow

1. Resuspend the Beads

Mix MNHS1.5UM-10 until the suspension is homogeneous.

Avoid excessive foaming.

2. Transfer the Required Amount

Calculate the bead amount according to:

• Number of tests

• Beads per test

• Required capture capacity

• Reaction volume

• Expected processing loss

3. Magnetically Collect the Beads

Place the tube in a compatible magnetic separator.

Wait until the beads are collected.

Remove the storage solution.

4. Wash with Coupling Buffer

Use a validated amine-free buffer.

Avoid Tris and glycine during coupling.

5. Prepare the Ligand

Transfer the antibody or protein into the coupling buffer.

Remove incompatible components when necessary.

Potential interfering components include:

• Tris

• Glycine

• Carrier proteins

• Free amino acids

• Amine-containing stabilizers

6. Add the Ligand

Add the antibody or protein to the prepared NHS magnetic beads.

Mix immediately.

7. Incubate

Maintain gentle mixing.

Optimize:

• Ligand concentration

• Bead concentration

• Buffer pH

• Reaction time

• Temperature

• Mixing speed

8. Wash the Coupled Beads

Magnetically collect the beads.

Remove unbound ligand.

Wash with a compatible buffer.

9. Quench Remaining NHS Groups

Use an application-compatible quenching reagent.

Validate the effect on background and ligand activity.

10. Block and Formulate

Add a suitable blocking and storage formulation.

Evaluate:

• Particle dispersion

• Magnetic response

• Antibody activity

• Non-specific binding

• Storage stability

Magnetic Immunoassay Workflow

1. Couple the capture antibody.

2. Quench remaining NHS groups.

3. Block and formulate the beads.

4. Add the test sample.

5. Incubate for target capture.

6. Magnetically separate the beads.

7. Wash away unbound sample.

8. Add the labeled detection antibody.

9. Incubate again.

10. Perform final magnetic washing.

11. Add the detection substrate.

12. Measure the signal.

Possible detection methods include:

• Chemiluminescence

• Electrochemiluminescence

• Fluorescence

• Enzyme colorimetry

• Other compatible reporter systems

Why Choose 1.5µm Magnetic Beads?

Compared with 200nm Beads

1.5µm beads generally provide:

• Larger surface per individual particle

• Easier magnetic collection

• Easier particle recovery

• Easier microscopic observation

The 200nm beads provide more particles and potentially more collective surface per unit mass.

Compared with Larger Magnetic Beads

1.5µm beads generally provide:

• More particles per unit mass

• Better suspension performance

• More capture surfaces

• Suitability for smaller reaction volumes

Larger beads may provide faster collection in some magnetic systems.

The final size should be selected through direct assay testing.

NHS Magnetic Beads vs Carboxyl Magnetic Beads

Choose NHS beads when rapid direct coupling is preferred.

Choose carboxyl beads when an established EDC/NHS process is already available.

Quality Control

Recommended tests include:

• Mean particle diameter

• Particle-size distribution

• Particle-size CV

• Suspension appearance

• Solids content

• NHS activity

• Magnetic response

• Magnetic recovery

• Redispersion

• Aggregate level

• Antibody coupling capacity

• Functional target binding

• Non-specific binding

• Batch consistency

Coupling Efficiency

Possible evaluation methods include:

• Protein mass balance

• Fluorescent ligand measurement

• Surface NHS activity testing

• Functional target-binding assays

Protein uptake does not always equal active antibody loading.

Functional immunoassay testing is essential.

Magnetic Performance

Evaluate:

• Collection time

• Bead recovery

• Residual beads in the supernatant

• Redispersion after magnetic capture

• Performance in the intended reaction vessel

• Compatibility with the intended analyzer

Bulk Manufacturing and Customization

Shanghai SanYu Biotechnology Co., Ltd. supports:

• Research samples

• Pilot batches

• Bulk manufacturing

• OEM production

• Private-label packaging

• Custom package sizes

• Custom NHS density

• Custom solids concentration

• Custom magnetic response

• Customer-specific quality standards

For project evaluation, provide:

• Required quantity

• Annual demand

• Intended immunoassay

• Ligand type

• Required coupling capacity

• Bead quantity per test

• Magnetic separator or analyzer

• Preferred package size

• Buffer restrictions

• Preservative restrictions

• Quality-control requirements

Handling and Storage

Follow the final SHBC product label, TDS and lot-specific COA.

General recommendations:

• Store under the specified refrigerated conditions.

• Do not freeze unless validated.

• Keep the container tightly closed.

• Mix before sampling.

• Do not allow the beads to dry.

• Use clean, low-binding tubes.

• Avoid amine-containing buffers before coupling.

• Protect NHS groups from moisture and hydrolysis.

• Use the prepared beads promptly.

• Do not return diluted material to the original bottle.

Frequently Asked Questions

What is MNHS1.5UM-10?

MNHS1.5UM-10 is a 1.5µm NHS-activated magnetic bead suspension supplied at 5% solids.

What is the main application?

It is designed for antibody and protein coupling in immunoassay research.

Is EDC/NHS activation required?

No additional EDC/NHS activation is normally required.

The surface is already NHS activated.

Which molecules can be coupled?

Antibodies, antigens, proteins, peptides, enzymes and other amine-containing molecules may be evaluated.

Which groups react with NHS?

NHS esters mainly react with primary amino groups.

Which buffers should be avoided?

Avoid Tris, glycine and other amine-containing buffers during coupling.

Is quenching required?

Quenching remaining NHS groups is generally recommended after ligand coupling.

Can MNHS1.5UM-10 be used in CLIA research?

Yes. It can be evaluated as a magnetic solid phase in chemiluminescent immunoassay development.

Can it be used on automated analyzers?

Yes, subject to validation of bead dispensing, magnetic collection, washing and redispersion.

What is the antibody coupling capacity?

Use the final product specification or lot-specific COA.

Do not estimate it from solids content alone.

How are the beads separated?

Use an external magnetic separator compatible with the reaction vessel and sample volume.

Can the beads be frozen?

Freezing is generally not recommended unless validated.

Can the bead pellet be allowed to dry?

No. Drying may cause aggregation and poor redispersion.

Is bulk production available?

Yes. SHBC supports samples, pilot batches, OEM projects and enterprise bulk manufacturing.

Request a Sample or Quotation

SHBC MNHS1.5UM-10 provides:

• 1.5µm nominal particle size

• NHS-activated surface

• 5% solids content

• Direct amine-based coupling

• Stable covalent ligand attachment

• Magnetic separation

• Immunoassay compatibility

• Automation-development potential

• Sample and bulk supply

• OEM and customization support

Product Name: 1.5µm NHS Magnetic Beads
Catalog Number: MNHS1.5UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 1.5µm
Surface: NHS activated
Solids Content: 5%
Application: Immunoassay research
Supply: Samples, pilot batches and bulk production
Intended Use: Research Use Only. Not for diagnostic or therapeutic use.