Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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MagNi3UM-10
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SHBC
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5%
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3µm
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10ml,20ml,50ml ,500ml,1000ml
3µm Nickel Magnetic Beads for Immunoassay Research
SHBC MNi3UM-10 Nickel Magnetic Beads are 3µm magnetic particles with a nickel-functional surface.
They are developed for the capture, purification and immobilization of His-tagged proteins.
The product is supplied at 5% solids for immunoassay research, recombinant antigen immobilization, magnetic affinity capture and bulk reagent production.
Product: 3µm Nickel Magnetic Beads
Catalog Number: MNi3UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 3µm
Surface: Nickel functionalized
Solids Content: 5%
Primary Target: His-tagged proteins
Main Application: Immunoassay research
Supply: Samples, pilot batches and bulk production
Intended Use: Research use only
Product Overview
MNi3UM-10 provides a magnetic affinity surface for His-tagged biomolecules.
Nickel ions interact with exposed histidine residues in a polyhistidine tag.
The captured protein can be:
Magnetically separated
Washed
Concentrated
Used directly on the beads
Eluted for downstream research
Nickel magnetic beads are commonly used for the purification and screening of His-tagged recombinant proteins. Magnetic processing removes the need for traditional chromatography columns in many small-scale workflows.
The 3µm particle size provides a balance between:
Magnetic recovery
Particle number
Surface per particle
Suspension contact
Repeated washing
Automated handling
MNi3UM-10 may also be used to immobilize His-tagged antigens or capture proteins for magnetic immunoassay development.
Technical Specifications
Parameter | Specification |
|---|---|
Product name | Nickel Magnetic Beads |
Catalog number | MNi3UM-10 |
Brand | SHBC |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Nominal diameter | 3µm |
Surface | Nickel functionalized |
Solids content | 5% |
Physical form | Magnetic bead suspension |
Separation method | External magnetic field |
Main binding target | His-tagged proteins |
Main application | Immunoassay and protein-affinity research |
Supply format | Samples and bulk supply |
Intended use | Research use only |
The following values should be confirmed by the final TDS or lot-specific COA:
Actual particle-size range
Particle-size CV
Particle morphology
Magnetic material
Magnetic material content
Magnetic response time
Nickel loading
Nickel leakage
Chelating ligand type
His-tag protein binding capacity
Particle concentration
Suspension buffer
Preservative
Package size
Shelf life
Storage temperature
The exact nickel-chelating ligand must be stated in the final specification.
Possible chelating ligands include:
NTA
IDA
Other nickel-chelating structures
Do not describe MNi3UM-10 as Ni-NTA unless NTA has been confirmed.
The 5% solids content alone cannot determine protein-binding capacity or magnetic response time.
Key Advantages
Fast His-Tag Protein Capture
The nickel-functional surface binds accessible histidine residues in His-tagged proteins.
This supports rapid magnetic affinity capture.
No Biotinylation Required
A His-tagged protein can bind through its affinity tag.
Biotinylation and streptavidin are not required.
No EDC/NHS Activation Required
Nickel–His tag binding is based on metal-affinity coordination.
The user does not normally need to activate carboxyl groups or perform covalent coupling.
Reversible Protein Binding
Captured His-tagged proteins may be released using a compatible competitive elution buffer.
Imidazole is commonly used to compete with histidine residues for nickel-binding sites.
3µm Particle Size
The 3µm diameter provides a larger surface on each bead than 1µm particles.
It may also support easier magnetic collection under suitable conditions.
Magnetic Separation
An external magnet collects the particles.
This supports:
Washing
Buffer exchange
Sample cleanup
Target concentration
Parallel processing
Automated handling
Suitable for Immunoassay Research
His-tagged recombinant antigens can be loaded onto the magnetic beads.
The antigen-loaded beads can then be evaluated in antibody-detection assays.
Suitable for Automation
MNi3UM-10 can be evaluated in:
Manual magnetic workflows
Semi-automated systems
Automated magnetic processors
Multiwell-plate workflows
High-throughput screening
5% Solids Suspension
The concentrated format supports:
Laboratory testing
Process optimization
Pilot manufacturing
Repeated reagent preparation
Enterprise bulk production
Bulk Manufacturing
SHBC supports:
Research samples
Pilot batches
Repeat orders
OEM production
Private-label packaging
Enterprise bulk supply
How Nickel–His Tag Binding Works
Nickel magnetic beads contain immobilized nickel ions held by a chelating surface.
The nickel ions coordinate with histidine residues in a polyhistidine tag.
The simplified structure is:
Magnetic Bead – Chelator – Ni²⁺ – His-Tagged Protein
A common affinity tag contains six histidine residues and is known as a 6×His tag.
The His tag may be located at the N-terminus or C-terminus of a recombinant protein.
Binding
Mix the His-tagged protein with equilibrated nickel magnetic beads.
The exposed histidine tag binds to available nickel sites.
Washing
Wash away non-bound and weakly bound sample components.
A low concentration of imidazole may help reduce non-specific protein binding.
The concentration must be optimized for the target protein.
Elution
A higher imidazole concentration can compete with the His tag and release the captured protein.
Lower pH or a compatible chelating condition may also release the protein, depending on the final bead chemistry.
On-Bead Use
Elution is not always necessary.
The His-tagged protein can remain on the beads for:
Immunoassay development
Antibody screening
Protein interaction research
Target capture
Biosensor research
Immunoassay Applications
His-Tagged Antigen Immobilization
A recombinant antigen containing a His tag can be captured on MNi3UM-10.
The antigen-loaded beads can be used for antibody-detection research.
Potential applications include:
Serological assay development
Antibody screening
Hybridoma screening
Vaccine-response research
Autoantibody research
Infectious-disease research
His-Tagged Capture Protein Immobilization
His-tagged receptors, antigens and binding proteins may be loaded onto the beads.
The prepared beads can capture a target analyte from the sample.
Chemiluminescent Immunoassay Research
MNi3UM-10 may be evaluated as a magnetic solid phase in CLIA development.
A general workflow includes:
Load a His-tagged capture protein.
Wash the beads.
Add the test sample.
Capture the target.
Perform magnetic washing.
Add the labeled detection reagent.
Wash again.
Add the chemiluminescent substrate.
Measure the signal.
Ligand retention, background and signal stability must be validated.
Fluorescent Immunoassays
The beads may be combined with fluorescent antibodies or probes.
Enzyme Immunoassays
The magnetic solid phase may be used with enzyme-labeled detection reagents.
Antibody Screening
His-tagged antigens can be immobilized for:
Monoclonal antibody screening
Polyclonal antibody testing
Antibody-specificity studies
Cross-reactivity evaluation
Binding comparison
Recombinant Protein Purification
MNi3UM-10 may be evaluated for His-tagged protein purification from:
Bacterial lysates
Yeast expression samples
Mammalian-cell samples
Insect-cell samples
Cell-free expression systems
Protein Interaction Research
His-tagged bait proteins may be immobilized for:
Protein–protein interaction studies
Ligand screening
Receptor-binding research
Pull-down research
Affinity capture
Suitable Target Molecules
MNi3UM-10 may be evaluated with:
6×His-tagged proteins
Polyhistidine-tagged proteins
His-tagged recombinant antigens
His-tagged antibodies
His-tagged antibody fragments
His-tagged enzymes
His-tagged receptors
His-tagged peptides
Histidine-rich affinity ligands
The His tag must remain accessible after protein folding.
A hidden or sterically blocked tag may reduce binding.
Important Limitation
Standard antibodies without a His tag should not be assumed to bind specifically.
For non-His-tagged antibodies, consider:
Carboxyl magnetic beads
NHS magnetic beads
Epoxy magnetic beads
Tosyl magnetic beads
Protein A/G magnetic beads
Streptavidin magnetic beads with biotinylated antibodies
Recommended Protein Binding Workflow
1. Resuspend the Beads
Mix MNi3UM-10 until homogeneous.
Use gentle inversion or controlled vortexing.
Avoid excessive foam.
2. Transfer the Required Amount
Calculate the bead amount according to:
Protein quantity
Required binding capacity
Number of tests
Reaction volume
Expected processing loss
3. Magnetically Collect the Beads
Place the tube in a compatible magnetic separator.
Wait until the beads have collected.
Remove the storage solution.
4. Equilibrate the Beads
Wash the beads with a compatible binding buffer.
The buffer may contain:
Phosphate or another compatible buffer
Sodium chloride
Low imidazole
Compatible nonionic detergent
Use the final SHBC protocol as the primary reference.
5. Prepare the Protein Sample
Check:
His-tag accessibility
Protein solubility
Sample pH
Salt concentration
Imidazole concentration
Chelator content
Reducing-agent content
Detergent compatibility
6. Add the Sample
Combine the sample with the equilibrated magnetic beads.
Mix gently.
7. Incubate
Optimize:
Binding time
Temperature
Mixing speed
Bead-to-protein ratio
Buffer pH
Salt concentration
Imidazole concentration
8. Magnetically Wash
Collect the beads with a magnet.
Remove unbound material.
Repeat washing as required.
9. Use or Elute the Protein
Use the protein-loaded beads directly in the assay.
Alternatively, elute the protein using a validated buffer.
10. Evaluate Performance
Measure:
Protein recovery
Protein purity
Binding capacity
Non-specific binding
Ligand activity
Magnetic recovery
Bead aggregation
Magnetic Immunoassay Workflow
Load the His-tagged antigen or capture protein.
Wash the magnetic beads.
Block the remaining surface when required.
Add the test sample.
Incubate for target binding.
Magnetically collect the beads.
Wash away unbound sample.
Add the labeled detection reagent.
Incubate again.
Perform final magnetic washing.
Add the detection substrate.
Measure the signal.
Possible detection systems include:
Chemiluminescence
Electrochemiluminescence
Fluorescence
Enzyme colorimetry
Other compatible reporters
Why Choose 3µm Magnetic Beads?
Larger Surface per Particle
Each 3µm bead provides more physical surface than each 1µm bead.
This may support higher ligand loading per individual bead.
Practical Magnetic Recovery
The larger particle size may support easier magnetic collection.
Actual performance depends on:
Magnetic material content
Magnet strength
Sample volume
Liquid height
Sample viscosity
Vessel geometry
Visible Magnetic Pellet
A 3µm bead population may form a more visible magnetic pellet than smaller particles.
This can simplify manual process development.
Repeated Washing
The beads can be collected and redispersed through multiple washing cycles.
Balanced Particle Size
Compared with 1µm magnetic beads, 3µm beads generally provide:
Larger surface per bead
Fewer particles per unit mass
Easier microscopic observation
Potentially easier magnetic collection
Faster settling
The final particle size should be selected through direct testing.
Nickel vs NHS and Streptavidin Magnetic Beads
Surface | Binding target | Main advantage | Main limitation |
|---|---|---|---|
Nickel | His-tagged proteins | Fast and reversible affinity capture | Target requires an accessible His tag |
NHS | Primary amines | Direct covalent protein coupling | NHS surface is sensitive to hydrolysis |
Carboxyl | Primary amines after activation | Flexible covalent coupling | Requires EDC/NHS activation |
Streptavidin | Biotinylated molecules | Fast modular loading | Target must be biotinylated |
Protein A/G | Antibody Fc region | Direct antibody capture | Species and isotype compatibility vary |
Choose nickel magnetic beads when:
The target protein has a His tag.
Reversible binding is useful.
Recombinant antigen immobilization is required.
His-tagged protein purification is needed.
Protein screening is planned.
Choose NHS or carboxyl beads when:
A non-His-tagged protein must be covalently immobilized.
Permanent ligand attachment is preferred.
Choose streptavidin beads when:
The ligand is biotinylated.
Rapid modular loading is required.
Quality Control
Recommended quality-control items include:
Mean particle diameter
Particle-size range
Particle-size CV
Particle morphology
Suspension appearance
Solids content
Magnetic material content
Magnetic response
Magnetic recovery
Redispersion
Aggregate level
Nickel loading
Nickel leakage
His-tag protein binding capacity
Non-specific binding
Functional antigen activity
Batch consistency
Protein-Binding Capacity
Binding capacity should be measured with a defined His-tagged protein.
The result depends on:
Protein size
His-tag position
Tag accessibility
Protein purity
Buffer composition
Incubation time
Test method
Do not estimate binding capacity from solids content alone.
Magnetic Performance
Evaluate:
Magnetic collection time
Bead recovery
Residual beads in the supernatant
Redispersion after collection
Performance in the intended vessel
Compatibility with the intended analyzer
Functional Immunoassay Testing
A model assay may evaluate:
Positive signal
Negative background
Signal-to-background ratio
Ligand retention
Dose-response performance
Precision
Washing efficiency
Lot-to-lot consistency
Bulk Manufacturing and Customization
Shanghai SanYu Biotechnology Co., Ltd. supports:
Research samples
Pilot batches
Bulk manufacturing
OEM production
Private-label packaging
Custom package sizes
Custom solids concentration
Custom nickel loading
Custom magnetic response
Customer-specific quality standards
For project evaluation, provide:
Required quantity
Annual demand
Intended application
His-tagged protein type
Required binding capacity
Bead amount per test
Magnetic separator or analyzer
Preferred package size
Buffer restrictions
Preservative restrictions
Quality-control requirements
Handling and Storage
Follow the final SHBC product label, TDS and lot-specific COA.
General recommendations:
Store under the specified conditions.
Do not freeze unless validated.
Keep the container tightly closed.
Mix before sampling.
Do not allow the beads to dry.
Use clean, low-binding tubes.
Maintain gentle mixing during binding.
Protect the product from contamination.
Do not return used material to the original bottle.
Buffer Compatibility
Nickel-affinity binding may be affected by:
EDTA
Other strong chelators
High imidazole concentrations
Extreme pH
Certain reducing agents
Compatibility depends on the chelating ligand used on MNi3UM-10.
Do not publish an exact compatibility table until SHBC testing is complete.
Avoid Drying
Do not allow the bead pellet to dry.
Drying may cause:
Aggregation
Poor redispersion
Reduced protein binding
Lower magnetic recovery
Higher assay variation
Frequently Asked Questions
What is MNi3UM-10?
MNi3UM-10 is a 3µm nickel-functional magnetic bead suspension supplied at 5% solids.
What is its main target?
It is designed primarily for His-tagged proteins.
What is a His tag?
A His tag is a short sequence containing multiple histidine residues.
A common format is the 6×His tag.
Can ordinary antibodies bind directly?
Ordinary antibodies should not be assumed to bind specifically.
The antibody should contain an accessible His tag or compatible histidine-rich sequence.
Can His-tagged antigens be immobilized?
Yes.
His-tagged recombinant antigens may be captured for immunoassay and antibody-screening research.
Is the binding covalent?
No.
Nickel–His tag binding is a reversible metal-affinity interaction.
Can the protein be eluted?
Yes.
His-tagged proteins may be eluted using a validated competitive condition, commonly involving imidazole.
Can the protein-loaded beads be used directly?
Yes.
The beads may be used directly in immunoassays, target capture and protein-interaction studies.
Can MNi3UM-10 be used in CLIA research?
Yes.
His-tagged antigens or capture proteins may be loaded onto the beads for magnetic CLIA development.
Can it be used for protein purification?
Yes.
It may be evaluated for magnetic purification and screening of His-tagged proteins.
Can it be used on automated instruments?
Yes, subject to validation of dispensing, magnetic collection, washing and redispersion.
What is the protein-binding capacity?
Use the final SHBC specification or lot-specific COA.
Do not estimate it from the 5% solids content.
Does the product use NTA?
The exact nickel-chelating ligand must be confirmed in the final TDS.
Do not describe the product as Ni-NTA unless NTA is confirmed.
Can EDTA be used?
EDTA may remove or compete with nickel ions in many nickel-affinity systems.
Compatibility must be confirmed for MNi3UM-10.
Can the beads be frozen?
Freezing is generally not recommended unless validated.
Can the bead pellet be allowed to dry?
No.
Drying may cause aggregation and poor redispersion.
Is bulk production available?
Yes.
SHBC supports samples, pilot batches, OEM projects and enterprise bulk manufacturing.
Request a Sample or Quotation
SHBC MNi3UM-10 provides:
3µm nominal particle size
Nickel-functional surface
5% solids content
His-tag protein capture
Magnetic separation
Recombinant antigen immobilization
Immunoassay research compatibility
CLIA development potential
Sample and pilot-batch supply
Enterprise bulk production
OEM and customization support
Product Name: 3µm Nickel Magnetic Beads
Catalog Number: MNi3UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 3µm
Surface: Nickel functionalized
Solids Content: 5%
Primary Target: His-tagged proteins
Application: Immunoassay and protein-affinity research
Supply: Samples, pilot batches and bulk production
Intended Use: Research Use Only. Not for diagnostic or therapeutic use.