50um Amino Magnetic Beads MNH50UM-10

50µm Amino Magnetic Beads MNH50UM-10

MNH50UM-10 is a 50µm amino-functional magnetic bead suspension supplied at a solids content of 5%.

The product is developed for the covalent immobilization of antibodies, antigens, proteins, peptides, enzymes and other affinity ligands used in immunoassay research, reagent development, process validation and batch manufacturing.

Primary amino groups on the bead surface provide reactive sites for biomolecule immobilization through a suitable activation or crosslinking system. After coupling, the functionalized beads can be used as a magnetic solid phase for target capture, magnetic separation, repeated washing and downstream signal detection.

MNH50UM-10 is intended for biotechnology companies, immunoassay developers, research institutions and reagent manufacturers requiring a defined 50µm magnetic carrier for laboratory evaluation, pilot production and bulk supply.

What Are 50µm Amino Magnetic Beads?

50µm amino magnetic beads are magnetic microspheres with a nominal particle diameter of approximately 50 micrometers and primary amino groups on the particle surface.

The amino groups serve as chemical reaction sites. They do not provide universal direct coupling to every antibody, protein or other biomolecule without an appropriate crosslinking or activation method.

Depending on the available functional groups of the selected ligand, developers may evaluate:

• Glutaraldehyde-mediated protein coupling;

• Activated carboxyl-to-amino coupling;

• Amine-to-sulfhydryl crosslinking;

• Aldehyde-to-amino coupling;

• Other heterobifunctional crosslinker systems.

Commercial amino magnetic bead series already include exact 50µm particle options, while other suppliers provide broader 30–150µm or 20–40µm ranges. This shows that larger amino-functional magnetic carriers are used when developers require different collection, washing, mixing or sample-processing characteristics from those provided by small micrometer or nanoscale beads.

Compared with smaller particles of similar composition, 50µm beads generally require greater attention to sedimentation, mixing and dispensing. They may also form a more clearly visible magnetic collection zone, which can simplify manual observation during washing and process development.

The suitability of MNH50UM-10 should be established using the intended ligand, assay format, sample matrix, magnetic separator and instrument platform.

MNH50UM-10 Product Specifications

The basis used to express the 5% solids content, together with the suspension medium, particle-size distribution, magnetic properties, amino-group density and storage conditions, should be confirmed using the applicable technical data sheet and lot-specific certificate of analysis.

Key Features of MNH50UM-10

Defined 50µm Particle Size

MNH50UM-10 provides a defined large-particle magnetic carrier rather than a broad particle-size range.

A specified nominal particle size can help developers establish consistent conditions for:

• Magnetic bead dispensing;

• Ligand coupling;

• Magnetic collection;

• Supernatant removal;

• Repeated washing;

• Bead resuspension;

• Automated processing;

• Lot-to-lot comparison.

Particle performance depends not only on nominal diameter but also on particle-size distribution, magnetic material content, density, surface coating, suspension medium and magnet configuration.

Amino-Functional Surface

Primary amino groups on the bead surface can participate in covalent biomolecule immobilization through compatible crosslinking chemistry.

Potential coupling targets include:

• Monoclonal antibodies;

• Polyclonal antibodies;

• Recombinant antigens;

• Natural proteins;

• Peptides;

• Enzymes;

• Streptavidin;

• Glycoproteins;

• Thiolated biomolecules;

• Carboxyl-containing ligands;

• Modified oligonucleotides;

• Other affinity molecules.

Commercial amino-functional magnetic particles are commonly used with glutaraldehyde, carbodiimide-related chemistry and other amine-reactive or heterobifunctional crosslinkers.

Concentrated 5% Solids Formulation

The 5% solids suspension gives developers flexibility to prepare different working concentrations during:

• Coupling-method screening;

• Ligand-loading optimization;

• Blocking studies;

• Immunoassay formulation;

• Stability evaluation;

• Pilot-scale production;

• Manufacturing scale-up.

Because 50µm particles may settle during storage and temporary processing holds, the suspension should be fully homogenized before sampling or dispensing.

Convenient Magnetic Solid-Phase Processing

After ligand immobilization, MNH50UM-10 can be collected with a compatible magnetic separator.

A typical magnetic assay workflow may include:

1. Addition of ligand-coupled magnetic beads;

2. Incubation with the sample;

3. Capture of the target analyte;

4. Magnetic collection;

5. Removal of unbound components;

6. Repeated washing;

7. Addition of detection reagents;

8. Signal measurement.

Suitable for Research-to-Production Projects

MNH50UM-10 can support projects involving:

• Initial feasibility evaluation;

• Coupling-chemistry screening;

• Immunoassay optimization;

• Sample-preparation development;

• Automated-system evaluation;

• Pilot-scale manufacturing;

• Stability studies;

• Batch-consistency testing;

• Bulk raw-material procurement.

Why Choose 50µm Magnetic Beads for Immunoassays?

Particle size affects bead number, available surface, sedimentation, mixing, magnetic collection, washing and instrument compatibility.

Clearly Observable Magnetic Collection

Under an appropriate magnetic field, larger micrometer-scale particles may form a clearly visible collection zone.

This can help developers monitor:

• Whether bead collection is complete;

• Whether beads are being lost during aspiration;

• Whether aggregation occurs;

• Whether washing is consistent;

• Whether the beads can be fully redispersed.

Actual collection behavior depends on the magnetic material content, bead concentration, liquid viscosity, vessel geometry and magnetic separator.

Practical Manual Washing

MNH50UM-10 may be evaluated when a manual or semi-automated process requires a larger carrier that can be easily observed during repeated collection and washing.

A clearly defined collection zone may simplify:

• Supernatant removal;

• Wash-buffer replacement;

• Process troubleshooting;

• Bead-recovery assessment;

• Operator training.

Potential Use in Sample Pretreatment

A larger magnetic carrier may be useful in selected sample-preparation, affinity-capture or target-enrichment workflows where particles must be isolated from complex or relatively viscous samples.

The development team should verify how the sample matrix affects:

• Particle aggregation;

• Sedimentation;

• Magnetic recovery;

• Nonspecific adsorption;

• Ligand accessibility;

• Bead redispersion.

Important Surface-Area Tradeoff

For particles with comparable composition and density, smaller diameters generally provide more surface area per unit mass. A 50µm bead process may therefore require different bead quantities and ligand-to-bead ratios from a process developed using 1µm, 2µm or 3µm beads.

The following parameters should be optimized specifically for MNH50UM-10:

• Bead dosage;

• Ligand input;

• Coupling time;

• Mixing conditions;

• Sample-incubation time;

• Washing sequence;

• Final reagent concentration.

Automated Instrument Compatibility

MNH50UM-10 may be evaluated on automated or semi-automated magnetic immunoassay systems, but compatibility should be confirmed experimentally.

Important instrument parameters include:

• Reagent-reservoir mixing;

• Pipette-tip internal diameter;

• Tubing dimensions;

• Dispensing accuracy;

• Reaction-cup geometry;

• Magnet position;

• Collection time;

• Aspiration height;

• Wash volume;

• Bead carryover;

• Redispersion efficiency.

A particle size suitable for one analyzer may not be suitable for another analyzer with a different fluidic or magnetic design.

Amino Surface Chemistry and Biomolecule Coupling

The most suitable coupling chemistry depends on the structure of the ligand, its available functional groups, the desired orientation and the required stability of the finished conjugate.

Glutaraldehyde-Mediated Coupling

Glutaraldehyde can be used as a bifunctional reagent to activate amino-functional magnetic beads before reaction with amino-containing proteins or antibodies.

Commercial amino magnetic bead instructions describe washing the particles, activating the amino surface with glutaraldehyde, adding the biological ligand while maintaining bead suspension, and subsequently blocking residual reactive sites.

Variables requiring optimization include:

• Glutaraldehyde concentration;

• Activation time;

• Reaction pH;

• Bead concentration;

• Antibody or protein input;

• Mixing method;

• Coupling time;

• Blocking formulation.

Excessive activation may contribute to bead-to-bead crosslinking, protein aggregation, reduced ligand activity or poor redispersion.

Activated Carboxyl-to-Amino Coupling

Carboxyl groups on a protein, peptide or other ligand can be activated before reaction with amino groups on MNH50UM-10.

PureCube’s amine-functional magnetic beads use this general approach for coupling biomolecules that contain accessible carboxyl groups.

Parameters that may require optimization include:

• Activation buffer;

• Reaction pH;

• Carbodiimide concentration;

• Optional NHS or Sulfo-NHS concentration;

• Ligand-to-bead ratio;

• Activation time;

• Coupling time;

• Quenching conditions.

Because a protein may contain several available carboxyl groups, coupling orientation can be heterogeneous. Functional binding performance should therefore be evaluated in addition to chemical coupling yield.

BS3 and Other Amine-Reactive Crosslinkers

BS3 is a water-soluble homobifunctional crosslinker with amine-reactive groups at both ends. It can connect primary amines on different molecules and may be evaluated in selected protein-coupling workflows.

Because both the magnetic bead surface and proteins may contain multiple amino groups, amine-to-amine chemistry can produce random ligand orientation.

Developers should evaluate:

• Crosslinker concentration;

• Ligand concentration;

• Reaction pH;

• Reaction time;

• Bead mixing;

• Blocking or quenching;

• Retained biological activity.

Sulfo-SMCC Amine-to-Sulfhydryl Coupling

Sulfo-SMCC is a heterobifunctional crosslinker containing an NHS ester and a maleimide group. The NHS ester reacts with primary amines, while the maleimide group reacts with free sulfhydryl groups.

This route may be considered when:

• The antibody or protein contains an accessible sulfhydryl group;

• The ligand has been selectively thiolated;

• Greater functional-group selectivity is required;

• Random amine-to-amine crosslinking is undesirable.

Buffers containing substances such as primary amines or free sulfhydryls can interfere with the applicable reaction step and should be selected carefully.

Aldehyde-to-Amino Coupling

Aldehyde-containing carbohydrates, glycoproteins or modified ligands may be evaluated for coupling to surface amino groups through reductive amination.

The buffer, reductant, pH, ligand concentration and reaction time should be optimized for the specific molecule.

Selecting the Coupling Route

The best coupling method should be selected according to functional immunoassay performance rather than coupling yield alone.

Applications in Immunoassay Reagent Development

Magnetic Sandwich Immunoassays

A capture antibody can be immobilized on MNH50UM-10.

The antibody-coupled beads are incubated with the sample to capture the target antigen. A labelled detection antibody can subsequently bind to the captured analyte and form a bead–target–detection antibody complex.

After magnetic collection and washing, the signal is measured using the selected detection chemistry.

Chemiluminescence Immunoassay Development

MNH50UM-10 may be evaluated as a magnetic solid-phase carrier in chemiluminescence immunoassay research.

Key variables include:

• Magnetic bead dosage;

• Capture-antibody loading;

• Blocking formulation;

• Sample-incubation time;

• Magnetic collection;

• Wash efficiency;

• Detection-antibody concentration;

• Enzyme-label compatibility;

• Chemiluminescent substrate;

• Signal-to-background ratio.

The larger bead size should be validated against the reaction vessel, washing system and fluidic path of the intended analyzer.

Electrochemiluminescence Immunoassay Research

The beads may also be evaluated in electrochemiluminescence immunoassay development where a larger magnetic carrier is compatible with the platform.

The development team should confirm compatibility with:

• The magnetic collection module;

• Electrode configuration;

• Electrochemiluminescent label;

• Reaction buffer;

• Assay consumables;

• Washing program;

• Signal-reading sequence.

Suitability for a specific ECL platform must be established experimentally.

Competitive Immunoassays

Antigens, antibodies, haptens or small-molecule conjugates may be immobilized on MNH50UM-10 for competitive assay development.

This format may be evaluated for analytes that cannot support a conventional two-site sandwich assay.

Antibody Detection

An antigen can be immobilized on the bead surface to capture target antibodies from research samples.

Immunomagnetic Separation

Ligand-functionalized MNH50UM-10 may be evaluated for magnetic enrichment or separation of:

• Proteins;

• Cells;

• Microorganisms;

• Biological particles;

• Other affinity-recognized targets.

Immunoprecipitation and Affinity Capture

Antibody- or protein-coupled beads may also be evaluated for immunoprecipitation, affinity capture and sample-preparation research.

Large amino-functional magnetic bead ranges are commercially positioned for the immobilization and separation of proteins, peptides, oligonucleotides and other biomolecules.

Recommended Antibody and Protein Coupling Workflow

The following workflow is a general development framework. It is not a validated product-specific protocol.

Step 1: Characterize the Ligand

Before coupling, record:

• Ligand type;

• Molecular weight;

• Concentration;

• Purity;

• Original buffer;

• Stabilizing additives;

• Available amino groups;

• Available carboxyl groups;

• Available sulfhydryl groups;

• Biological activity.

The original ligand formulation may require buffer exchange when it contains components that interfere with the selected crosslinker.

Step 2: Select the Coupling Chemistry

Choose the reaction according to the ligand and intended conjugate:

• Glutaraldehyde-mediated coupling;

• Activated carboxyl-to-amino coupling;

• BS3 or another amine-reactive crosslinker;

• Sulfo-SMCC amine-to-sulfhydryl coupling;

• Reductive amination;

• Another functional-group-specific method.

Step 3: Fully Resuspend MNH50UM-10

Mix the original suspension until it is homogeneous before removing an aliquot.

Suitable methods may include:

• Gentle inversion;

• Roller mixing;

• End-over-end rotation;

• Controlled vortexing;

• Validated low-energy sonication.

Because 50µm particles can settle, maintain suitable mixing while preparing multiple aliquots from the same container.

Step 4: Determine the Required Bead Quantity

Evaluate several bead quantities rather than relying on one fixed dosage.

The optimal amount depends on:

• Target concentration;

• Ligand-loading level;

• Sample volume;

• Required sensitivity;

• Sample matrix;

• Magnetic separator;

• Assay format.

Step 5: Wash and Exchange the Buffer

Collect the beads using a compatible magnetic separator.

Remove the original suspension medium and wash the particles using a buffer suitable for the selected coupling reaction.

Do not allow the collected beads to dry. Commercial magnetic bead suppliers warn that drying or freezing can cause aggregation and reduce effective bead performance.

Step 6: Activate the Beads or Ligand

Control the following parameters:

• Crosslinker concentration;

• Bead concentration;

• Activation pH;

• Reaction time;

• Temperature;

• Mixing rate;

• Reagent-addition order.

Step 7: Add the Antibody or Protein

Evaluate multiple ligand-to-bead ratios.

Excessive ligand input may increase raw-material cost or create surface crowding without improving target-binding performance.

Step 8: Maintain a Uniform Suspension

Use gentle continuous or intermittent mixing during activation and coupling.

This is especially important for 50µm beads because settling can produce uneven surface exposure, variable ligand loading and inconsistent batch results.

Step 9: Quench or Block Residual Sites

After coupling, add an appropriate quenching or blocking reagent.

The selected blocker should reduce nonspecific interactions without interfering with target recognition or downstream detection chemistry.

Step 10: Wash the Coupled Beads

Remove:

• Unbound ligand;

• Residual crosslinker;

• Reaction by-products;

• Excess blocking reagent.

Use consistent magnet type, collection time and aspiration settings.

Step 11: Resuspend in Storage Buffer

Potential formulation variables include:

• Buffer type;

• pH;

• Ionic strength;

• Protein stabilizer;

• Synthetic polymer;

• Surfactant;

• Preservative;

• Sugar or polyol;

• Final bead concentration.

Step 12: Evaluate Coupling Performance

Recommended evaluation items include:

• Ligand depletion from the supernatant;

• Chemical coupling yield;

• Functional target-binding capacity;

• Nonspecific binding;

• Magnetic recovery;

• Bead redispersion;

• Immunoassay signal;

• Signal-to-background ratio;

• Precision;

• Accelerated stability;

• Real-time stability.

How to Optimize MNH50UM-10 in an Immunoassay

Optimize Bead Dosage

Test several bead quantities in the finished assay.

Insufficient beads may limit target capture, while excessive beads may increase sedimentation, reagent consumption, background or washing requirements.

Optimize Ligand Density

Prepare conjugates with low, medium and high ligand-loading levels.

Compare:

• Coupling yield;

• Functional binding;

• Blank signal;

• Positive signal;

• Signal-to-background ratio;

• Analytical sensitivity;

• Precision;

• Storage stability.

The condition with the highest measured protein loading is not necessarily the condition with the best assay performance.

Optimize Mixing Conditions

Because 50µm particles can settle during processing, evaluate:

• Continuous versus intermittent mixing;

• Rotation speed;

• Shaking speed;

• Mixing duration;

• Vessel geometry;

• Foam formation;

• Ligand stability;

• Bead uniformity during sampling.

Optimize Magnetic Collection

Confirm:

• Magnet strength;

• Collection time;

• Container geometry;

• Sample volume;

• Collection-zone position;

• Residual liquid volume;

• Bead loss during aspiration;

• Recovery after repeated washing.

Optimize Redispersion

Ensure that the collected particles can be completely and consistently redispersed.

Incomplete redispersion may cause:

• Variable bead dosage;

• Reduced target capture;

• Increased precision error;

• Uneven reagent filling;

• Instrument blockage;

• Inconsistent analytical signal.

Evaluate Matrix Interference

Depending on the intended immunoassay, interference testing may include:

• Hemoglobin;

• Bilirubin;

• Lipids;

• Rheumatoid factor;

• Heterophilic antibodies;

• Biotin;

• Anticoagulants;

• High concentrations of unrelated proteins;

• Sample preservatives.

Evaluate Instrument Compatibility

For automated systems, evaluate:

• Reagent-reservoir mixing;

• Bead settling during standby;

• Dispensing accuracy;

• Tip and tubing dimensions;

• Magnetic collection;

• Aspiration height;

• Wash efficiency;

• Bead carryover;

• Resuspension after washing.

Quality Control for Research and Batch Production

MNH50UM-10 should be evaluated using both physical characterization and functional immunoassay testing.

Appearance and Dispersion

Inspect:

• Suspension appearance;

• Visible aggregation;

• Sedimentation behavior;

• Homogeneity after mixing;

• Ease of redispersion;

• Stability during process holds.

Particle Size and Distribution

Confirm the nominal 50µm particle size using an appropriate analytical method.

For manufacturing projects, monitor both average size and particle-size distribution.

Solids Content

Verify solids content because it affects:

• Bead dosage;

• Ligand consumption;

• Coupling calculations;

• Production yield;

• Final reagent concentration.

Amino Surface Reactivity

A standardized model ligand or crosslinking reaction can be used to evaluate the functional reactivity of the amino surface.

Magnetic Collection Performance

Use standardized conditions, including:

• Magnet type;

• Container;

• Sample volume;

• Buffer composition;

• Bead concentration;

• Collection time;

• Temperature.

Coupling Performance

Conduct a standardized coupling test using a representative antibody or protein.

Potential analytical approaches include:

• Protein depletion from the supernatant;

• Colorimetric protein assays;

• Fluorescent ligand measurements;

• Functional target-binding tests;

• Immunoassay signal evaluation.

Nonspecific Binding

Compare:

• Untreated beads;

• Activated beads;

• Blocked beads;

• Ligand-coupled beads;

• Negative sample matrices;

• Potential interfering substances.

Functional Immunoassay Testing

Recommended tests include:

• Blank signal;

• Negative-sample signal;

• Positive-sample signal;

• Signal-to-background ratio;

• Analytical sensitivity;

• Precision;

• Recovery;

• Linearity;

• Specificity;

• Hook effect;

• Stability.

Lot-to-Lot Consistency

For routine manufacturing, establish acceptance ranges for critical physical and functional parameters.

Final raw-material approval should be based on performance in the complete immunoassay system rather than particle characterization alone.

Scale-Up and Bulk Supply for Immunoassay Manufacturers

MNH50UM-10 can support projects progressing from laboratory evaluation to batch manufacturing.

Evaluation Stage

Evaluation samples may be used to screen:

• Coupling chemistry;

• Crosslinker concentration;

• Ligand-to-bead ratio;

• Blocking formulation;

• Mixing conditions;

• Magnetic separation;

• Assay sensitivity;

• Background signal.

Pilot-Scale Stage

Pilot quantities can support:

• Coupling-process confirmation;

• Reagent formulation;

• Automated-platform testing;

• Stability studies;

• Filling-process evaluation;

• Initial lot-consistency testing.

Batch-Production Stage

Bulk supply planning may include:

• Annual demand;

• Forecasted purchasing schedule;

• Packaging volume;

• Quality specifications;

• Technical documentation;

• Reserved-lot requirements;

• Change-control expectations;

• Safety-stock planning.

Scale-Up Considerations

A laboratory process should not be enlarged only by multiplying every reagent volume.

The following factors must also be revalidated:

• Reaction-vessel geometry;

• Mixing efficiency;

• Bead-suspension uniformity;

• Reagent-addition sequence;

• Temperature distribution;

• pH control;

• Magnetic collection;

• Washing efficiency;

• Process hold time;

• Final filling homogeneity.

For 50µm beads, maintaining a uniform suspension during coupling, storage and filling is particularly important.

Custom Amino Magnetic Bead Services

SHBC can evaluate customized requirements for biotechnology companies, research institutions and immunoassay reagent manufacturers.

Potential customization options include:

• Alternative particle sizes;

• Customized solids content;

• Adjusted amino-group density;

• Customized suspension buffer;

• Alternative preservative systems;

• Customer-specified pH;

• Special packaging volumes;

• Pilot-scale manufacturing;

• Bulk manufacturing;

• OEM packaging;

• Private-label service;

• Customer-specific quality testing.

Customization feasibility depends on the requested specifications, intended application, validation requirements and order quantity.

Storage and Handling Recommendations

Store MNH50UM-10 according to the product label, technical data sheet and lot-specific certificate of analysis.

General recommendations include:

• Mix thoroughly before sampling;

• Maintain suspension uniformity during dispensing;

• Use clean and calibrated pipetting equipment;

• Avoid allowing the beads to dry;

• Prevent microbial and chemical contamination;

• Confirm buffer compatibility before buffer exchange;

• Avoid freezing unless specifically validated;

• Avoid unnecessary exposure to extreme temperatures;

• Record the product lot number;

• Evaluate finished-conjugate stability separately.

Other commercial amino magnetic bead suppliers similarly recommend thorough mixing before use and caution against freezing or drying because these conditions may promote aggregation and make complete redispersion difficult.

Frequently Asked Questions

What is MNH50UM-10?

MNH50UM-10 is a 50µm amino-functional magnetic bead suspension supplied at 5% solids for biomolecule coupling, immunoassay development, process scale-up and batch production.

What functional group is present on the bead surface?

The particle surface contains primary amino groups, commonly written as NH₂ groups.

Which biomolecules can be coupled to MNH50UM-10?

Potential coupling targets include antibodies, antigens, proteins, peptides, enzymes, streptavidin, glycoproteins and suitably modified oligonucleotides.

Can antibodies be coupled directly to amino magnetic beads?

A suitable activation or crosslinking system is normally required. The optimal method depends on the available functional groups of the antibody and the desired conjugate structure.

Which coupling methods can be evaluated?

Potential routes include glutaraldehyde-mediated coupling, activated carboxyl-to-amino coupling, BS3-type amine crosslinking, Sulfo-SMCC amine-to-thiol coupling and reductive amination.

Why choose a 50µm particle size?

The 50µm format may be selected when a process requires a larger magnetic carrier, clearly observable collection, repeated washing and convenient manual handling.

How do 50µm beads differ from smaller magnetic beads?

Compared with smaller particles of similar composition, 50µm beads generally provide fewer individual particles and less geometric surface area per unit mass, while offering different settling, collection and washing behavior.

Do 50µm magnetic beads settle?

Large particles may settle during storage, dispensing and incubation. The suspension should be thoroughly mixed before sampling and maintained under suitable mixing conditions during processing.

Can MNH50UM-10 be used for chemiluminescence immunoassays?

MNH50UM-10 can be evaluated as a magnetic solid-phase carrier in chemiluminescence immunoassay development. Compatibility with the complete reagent and instrument system must be validated.

Can it be used for electrochemiluminescence immunoassays?

The product may be evaluated in electrochemiluminescence immunoassay research. Compatibility with the magnetic module, electrode, label chemistry, fluidic path and assay formulation should be confirmed experimentally.

Does 5% solids mean the beads are ready to use without dilution?

No. The 5% suspension is a concentrated raw material. The optimal working concentration should be determined during coupling and assay development.

Does higher antibody loading always improve sensitivity?

No. Excessive ligand density may cause steric hindrance, reduced target accessibility or increased nonspecific binding.

How can coupling efficiency be evaluated?

One approach is to compare the initial ligand concentration with the unbound ligand remaining in the post-coupling supernatant. Functional target-binding activity should also be measured.

How should MNH50UM-10 be mixed before use?

Mix until homogeneous using a validated method such as inversion, roller mixing, end-over-end rotation or gentle controlled vortexing.

What should be checked if magnetic collection is incomplete?

Check magnet strength, collection time, bead concentration, liquid viscosity, particle aggregation and vessel geometry.

Can MNH50UM-10 be used with automated immunoassay analyzers?

It can be evaluated for automated systems, but dispensing, mixing, magnetic collection, aspiration, washing and redispersion must be validated on the specific platform.

Is MNH50UM-10 a finished diagnostic reagent?

No. MNH50UM-10 is supplied as a raw material for research and reagent development. The customer is responsible for validating the finished reagent, manufacturing process and intended application.

Are evaluation samples available?

Evaluation samples can be discussed according to the intended application, coupling target and required test quantity.

Is bulk supply available?

Pilot-scale and bulk supply can be arranged for qualified research, development and manufacturing projects.

Can the concentration, buffer or packaging be customized?

Customized solids content, suspension buffer, amino surface parameters and packaging formats may be evaluated according to project requirements.

Request a Sample or Bulk Quotation

Contact SHBC to request an evaluation sample, technical information or bulk quotation for MNH50UM-10 50µm Amino Magnetic Beads.

Please provide:

• Intended immunoassay application;

• Target antibody, antigen or protein;

• Preferred coupling method;

• Required evaluation quantity;

• Estimated pilot quantity;

• Expected annual demand;

• Preferred packaging volume;

• Required technical documents;

• Required quality documents;

• Target development schedule;

• Custom specification requirements.

SHBC supports amino magnetic bead projects from early-stage coupling evaluation and immunoassay optimization to pilot production and bulk manufacturing.