5umTosyl Magnetic Beads MTOS5UM-10 5%

5µm Tosyl Magnetic Beads for CLIA Development

SHBC MTOS5UM-10 Tosyl Magnetic Beads are 5µm magnetic particles with a Tosyl-functional surface.

They are developed for covalent coupling of antibodies, antigens, proteins, peptides and enzymes.

The product is supplied at 5% solids for chemiluminescent immunoassay research, magnetic washing and bulk reagent production.

Product: 5µm Tosyl Magnetic Beads
Catalog Number: MTOS5UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 5µm
Surface: Tosyl functionalized
Solids Content: 5%
Main Application: CLIA and immunoassay research
Supply: Samples, pilot batches and bulk production
Intended Use: Research use only

Product Overview

MTOS5UM-10 provides a reactive magnetic solid phase.

The Tosyl surface can immobilize antibodies, antigens, proteins, peptides and enzymes.

Coupling may occur through accessible primary amino or sulfhydryl groups.

After coupling, the functionalized beads can capture target molecules from biological samples.

An external magnet separates the bead complexes from unbound reagents.

The 5µm particle size provides a relatively large surface on each individual bead.

It may also support practical magnetic recovery and repeated washing.

Technical Specifications

The following values should be confirmed by the final TDS or lot-specific COA:

• Actual particle-size range

• Particle-size CV

• Particle morphology

• Particle material

• Magnetic material

• Magnetic content

• Magnetic response time

• Tosyl-group density

• Antibody coupling capacity

• Protein coupling capacity

• Suspension buffer

• Preservative

• Package size

• Shelf life

• Storage temperature

The 5% solids content alone cannot determine coupling capacity or magnetic response time.

Key Advantages

Tosyl-Functional Surface

The particle surface is prepared for covalent ligand-coupling development.

It can react with suitable amino- or thiol-containing biomolecules.

Stable Covalent Coupling

Covalent immobilization helps reduce ligand loss during washing and storage.

This supports stable magnetic immunoassay reagent development.

Suitable for Antibody Coupling

Monoclonal and polyclonal antibodies may be immobilized.

The final coupling conditions should preserve antigen-binding activity.

5µm Particle Size

The 5µm diameter provides a large surface on each individual bead.

It also makes the particles easier to observe than nanoscale magnetic beads.

Magnetic Separation

The beads can be collected with an external magnetic field.

This supports:

• Sample washing

• Buffer exchange

• Target enrichment

• Reporter removal

• Automated processing

Suitable for CLIA

MTOS5UM-10 can be evaluated as a magnetic solid phase in CLIA and CLEIA development.

Tosyl magnetic beads have been used in automated chemiluminescent immunoassay coating-process optimization.

Automation Potential

The magnetic format can be evaluated in:

• Manual assays

• Semi-automated systems

• Automated CLIA analyzers

• High-throughput magnetic workflows

5% Solids Suspension

The concentrated format supports:

• Laboratory testing

• Coupling optimization

• Pilot manufacturing

• Bulk reagent preparation

Bulk Manufacturing

SHBC supports:

• Research samples

• Pilot batches

• Repeat orders

• OEM production

• Private-label packaging

• Enterprise bulk supply

How Tosyl Coupling Works

Tosyl groups can react with suitable nucleophilic groups on biomolecules.

Common reactive groups include:

• Primary amino groups

• Sulfhydryl groups

These groups may be present on:

• Antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Lectins

• Modified biomolecules

The simplified process is:

Tosyl Magnetic Bead + Protein Ligand → Covalently Coupled Bead–Ligand

Thermo Fisher describes Tosyl-activated magnetic beads as reactive toward primary amino and sulfhydryl groups. No additional bead-surface activation is required before ligand coupling.

Factors Affecting Coupling

Coupling performance depends on:

• Ligand type

• Ligand concentration

• Available reactive groups

• Buffer pH

• Ionic strength

• Coupling temperature

• Incubation time

• Mixing method

• Bead concentration

• Tosyl-group density

Buffer Selection

Use a coupling buffer compatible with the ligand.

Avoid buffers containing reactive components that may compete with the ligand.

Potential interfering components include:

• Tris

• Glycine

• Free proteins

• Free amino acids

• Thiol-containing reducing agents

Coupling Time

Tosyl coupling normally requires longer incubation than simple adsorption.

Established protocols often use extended incubation with continuous gentle mixing. Exact conditions should be optimized for the selected ligand.

Ligand Orientation

Coupling through naturally occurring amino groups may produce mixed ligand orientations.

High protein loading does not always produce the highest assay signal.

Functional target-binding tests are essential.

CLIA and Immunoassay Applications

Chemiluminescent Sandwich Immunoassays

Couple a capture antibody to MTOS5UM-10.

The antibody-coated beads capture the target antigen.

A labeled detection antibody then generates the signal.

Potential research targets include:

• Hormones

• Cytokines

• Tumor biomarkers

• Cardiac biomarkers

• Inflammatory markers

• Infectious-disease antigens

• Autoantibodies

• Veterinary biomarkers

Chemiluminescent Enzyme Immunoassays

The beads may be used with enzyme-labeled detection antibodies.

After magnetic washing, a compatible chemiluminescent substrate is added.

Competitive Immunoassays

Potential applications include:

• Drugs

• Haptens

• Hormones

• Toxins

• Metabolites

• Small molecules

• Peptides

Antigen-Coated Magnetic Beads

Recombinant antigens and proteins may be coupled for:

• Antibody detection

• Serological assay research

• Autoantibody research

• Vaccine-response studies

• Antibody screening

Antibody-Coated Magnetic Beads

Coupled antibodies may be used for:

• Antigen capture

• Biomarker enrichment

• Immunoprecipitation research

• Protein purification research

• Protein-complex capture

Peptide Coupling

Peptides with suitable reactive groups may be immobilized for:

• Epitope mapping

• Antibody screening

• Competitive assays

• Receptor-binding research

Enzyme Immobilization

Functional enzymes may be coupled for:

• Enzyme assays

• Biosensor research

• Biocatalysis

• Reporter-system development

Affinity Capture

The beads may also be evaluated for:

• Target enrichment

• Protein separation

• Immunoprecipitation

• Antigen purification

• Protein interaction studies

Suitable Coupling Molecules

MTOS5UM-10 may be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant antibodies

• Antibody fragments

• Recombinant antigens

• Proteins

• Peptides

• Enzymes

• Lectins

• Receptors

• Thiol-modified ligands

• Amino-containing ligands

The final coupling process depends on the selected molecule.

Recommended Coupling Workflow

1. Resuspend the Beads

Mix MTOS5UM-10 until homogeneous.

Avoid excessive foaming.

2. Transfer the Required Amount

Calculate the bead quantity according to:

• Number of tests

• Bead mass per test

• Required capture capacity

• Reaction volume

• Expected processing loss

3. Magnetically Collect the Beads

Place the tube in a compatible magnetic separator.

Remove the storage solution after bead collection.

4. Wash the Beads

Wash with the selected coupling buffer.

Use a buffer compatible with the ligand and Tosyl coupling.

5. Prepare the Ligand

Prepare the antibody, antigen, protein, peptide or enzyme.

Check:

• Purity

• Concentration

• Aggregation

• Buffer composition

• Biological activity

6. Add the Ligand

Combine the ligand with the washed magnetic beads.

Mix gently.

7. Incubate

Optimize:

• Ligand-to-bead ratio

• Buffer pH

• Salt concentration

• Temperature

• Coupling time

• Mixing speed

8. Wash the Coupled Beads

Magnetically collect the beads.

Remove unbound ligand.

Wash with a compatible buffer.

9. Block Remaining Surface

Use a suitable blocking formulation.

This helps reduce non-specific adsorption.

10. Formulate the Final Reagent

Evaluate:

• Particle dispersion

• Magnetic response

• Ligand activity

• Assay background

• Storage stability

Magnetic Immunoassay Workflow

1. Couple the capture antibody.

2. Wash and block the beads.

3. Add the test sample.

4. Incubate for target capture.

5. Magnetically collect the beads.

6. Wash away unbound sample.

7. Add the labeled detection antibody.

8. Incubate again.

9. Perform final magnetic washing.

10. Add the chemiluminescent substrate.

11. Measure the signal.

Possible detection systems include:

• Chemiluminescence

• Electrochemiluminescence

• Fluorescence

• Enzyme colorimetry

Why Choose 5µm Magnetic Beads?

Larger Surface per Individual Bead

Each 5µm bead provides more physical surface than each smaller magnetic particle.

This may support stronger signal generation per individual bead.

Practical Magnetic Recovery

The larger particle size may support easier magnetic collection under suitable conditions.

Actual magnetic response depends on:

• Magnetic material content

• Magnet strength

• Bead concentration

• Sample volume

• Sample viscosity

• Vessel geometry

Visible Magnetic Pellet

A larger bead population may form a more visible pellet during magnetic separation.

This can simplify manual process development.

Repeated Washing

The magnetic beads can be collected and resuspended through multiple wash cycles.

Microscopic Observation

The 5µm particles are easier to observe by microscopy than nanoscale beads.

Important Trade-Off

Compared with smaller beads, 5µm particles generally provide:

• Fewer particles per unit mass

• Less collective surface area per unit mass

• Faster settling

• Larger surface per individual bead

• Easier visual observation

The final particle size should be selected through direct assay testing.

Tosyl vs Epoxy and Carboxyl Magnetic Beads

Choose Tosyl magnetic beads when:

• Stable antibody or protein immobilization is required.

• The ligand contains accessible amino or thiol groups.

• A longer controlled coupling process is acceptable.

• CLIA or automated magnetic immunoassays are being developed.

Choose carboxyl magnetic beads when:

• A validated EDC/NHS process is already available.

• Amide coupling is preferred.

• Activation conditions can be tightly controlled.

Quality Control

Recommended quality-control items include:

• Mean particle diameter

• Particle-size range

• Particle-size CV

• Particle morphology

• Suspension appearance

• Solids content

• Tosyl-group activity

• Magnetic response

• Magnetic recovery

• Redispersion

• Aggregate level

• Antibody coupling capacity

• Functional target binding

• Non-specific binding

• CLIA background

• Batch consistency

Coupling Efficiency

Possible evaluation methods include:

• Protein mass balance

• Fluorescent ligand measurement

• Surface Tosyl activity testing

• Functional target-binding assay

Protein uptake does not always equal active antibody loading.

Functional Testing

A model CLIA may evaluate:

• Positive signal

• Negative background

• Signal-to-background ratio

• Dose-response behavior

• Precision

• Recovery

• Magnetic wash efficiency

• Lot-to-lot consistency

Magnetic Performance

Evaluate:

• Magnetic collection time

• Bead recovery

• Residual beads in the supernatant

• Redispersion after magnetic collection

• Performance in the intended reaction vessel

• Compatibility with the intended analyzer

Bulk Manufacturing and Customization

Shanghai SanYu Biotechnology Co., Ltd. supports:

• Research samples

• Pilot batches

• Bulk manufacturing

• OEM production

• Private-label packaging

• Custom package sizes

• Custom Tosyl-group density

• Custom solids concentration

• Custom magnetic response

• Customer-specific quality standards

For project evaluation, provide:

• Required quantity

• Annual demand

• Intended CLIA application

• Ligand type

• Required coupling capacity

• Bead amount per test

• Required magnetic response

• Automated analyzer model

• Preferred package size

• Buffer restrictions

• Preservative restrictions

• Quality-control requirements

Handling and Storage

Follow the final SHBC product label, TDS and lot-specific COA.

General recommendations:

• Store under the specified conditions.

• Do not freeze unless validated.

• Keep the container tightly closed.

• Mix before sampling.

• Do not allow the beads to dry.

• Use clean, low-binding tubes.

• Protect the surface from contamination.

• Maintain gentle mixing during coupling.

• Do not return diluted material to the original bottle.

Resuspension

Before use:

1. Gently invert the bottle.

2. Apply controlled vortexing when necessary.

3. Confirm a uniform suspension.

4. Mix again before each aliquot.

5. Inspect for irreversible aggregates.

Avoid Drying

Do not allow the magnetic bead pellet to dry.

Drying may cause:

• Aggregation

• Poor redispersion

• Reduced coupling performance

• Increased background

• Poor automation performance

Frequently Asked Questions

What is MTOS5UM-10?

MTOS5UM-10 is a 5µm Tosyl-functional magnetic bead suspension supplied at 5% solids.

What is the main application?

It is designed for antibody and protein coupling in CLIA and immunoassay research.

Which molecules can be coupled?

Antibodies, antigens, proteins, peptides, enzymes and other suitable amino- or thiol-containing molecules may be evaluated.

Which groups react with Tosyl?

Tosyl surfaces can form covalent links with suitable primary amino and sulfhydryl groups.

Is additional surface activation required?

The Tosyl functionality is already present.

The user should optimize the ligand-coupling process rather than perform EDC/NHS activation.

Can MTOS5UM-10 be used in CLIA?

Yes. It can be evaluated as a magnetic solid phase in chemiluminescent immunoassay development.

Can it be used on automated analyzers?

Yes, subject to validation of dispensing, magnetic collection, washing, redispersion and carryover.

Is blocking required?

Blocking is generally evaluated after coupling to reduce non-specific adsorption.

What is the antibody coupling capacity?

Use the final product specification or lot-specific COA.

Do not estimate coupling capacity from solids content alone.

How are the beads separated?

Use an external magnetic separator compatible with the vessel and sample volume.

Can the beads be frozen?

Freezing is generally not recommended unless validated.

Can the bead pellet be allowed to dry?

No. Drying may cause aggregation and poor redispersion.

Is bulk production available?

Yes. SHBC supports samples, pilot batches, OEM projects and enterprise bulk manufacturing.

Request a Sample or Quotation

SHBC MTOS5UM-10 provides:

• 5µm nominal particle size

• Tosyl-functional surface

• 5% solids content

• Covalent antibody and protein coupling

• Magnetic separation

• CLIA and CLEIA compatibility

• Automation-development potential

• Sample and pilot-batch supply

• Enterprise bulk production

• OEM and customization support

Product Name: 5µm Tosyl Magnetic Beads
Catalog Number: MTOS5UM-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 5µm
Surface: Tosyl functionalized
Solids Content: 5%
Application: CLIA and immunoassay research
Supply: Samples, pilot batches and bulk production
Intended Use: Research Use Only. Not for diagnostic or therapeutic use.