H-Thr-OL-2-CT Resin 100-200mesh,1% DVB

H-Thr-ol-2-Chlorotrityl Resin for Peptide Alcohol Synthesis

SHBC H-Thr-ol-2-Chlorotrityl Resin is a threoninol-preloaded solid support designed for the solid-phase synthesis of peptides containing a C-terminal threoninol or amino-alcohol functionality.

The threoninol building block is attached to an acid-labile 2-chlorotrityl linker on a 1% DVB-crosslinked polystyrene matrix. Its free amino group is available for direct coupling with the next Fmoc-protected amino acid.

The 100–200 mesh particle size provides practical swelling, reagent accessibility, filtration and washing performance.

Product Specifications

This specification assumes that the threoninol hydroxyl group is not tert-butyl protected. Please refer to the SHBC COA for the exact protection state and stereochemical designation.

Key Advantages of SHBC H-Thr-ol-2-CT Resin

Direct Synthesis of Peptide Alcohols

The preloaded threoninol building block allows direct preparation of peptides with a C-terminal amino-alcohol structure.

Eliminates In-House Threoninol Loading

The resin is supplied with threoninol already immobilized, reducing loading steps and simplifying synthesis preparation.

Free Amine for Direct Coupling

The H-Thr-ol amino group is unprotected and ready for coupling with the next Fmoc-protected amino acid.

Mild Acidic Cleavage

The 2-chlorotrityl linkage can be cleaved under mild acidic conditions, supporting the recovery of protected or acid-sensitive peptide fragments.

Avoids Post-Synthesis Carboxyl Reduction

Direct use of a threoninol-preloaded support can reduce the need to convert a C-terminal peptide carboxylic acid into an alcohol after synthesis.

Practical Resin Matrix

The 1% DVB-crosslinked polystyrene and 100–200 mesh particle size provide a balance between swelling, reagent diffusion, filtration and mechanical handling.

How H-Thr-ol-2-CT Resin Works

The terminal hydroxyl group of threoninol is immobilized on the 2-chlorotrityl support through an acid-labile linkage. Its amino group remains available for peptide-chain assembly.

A typical workflow includes:

1. Pre-swell the resin in a compatible SPPS solvent.

2. Couple the first Fmoc-protected amino acid to the free threoninol amino group.

3. Remove the Fmoc protecting group.

4. Continue repeated deprotection and coupling cycles.

5. Wash the resin between reaction steps.

6. Cleave the peptide from the resin using validated mild-acid conditions.

7. Purify and characterize the resulting peptide alcohol.

Cleavage conditions must be optimized according to the peptide sequence, protecting groups and required final protection state.

Applications

SHBC H-Thr-ol-2-Chlorotrityl Resin can be used for:

• C-terminal peptide alcohol synthesis

• Peptidyl threoninol preparation

• Fmoc solid-phase peptide synthesis

• Protected peptide fragments

• Acid-sensitive peptide intermediates

• Peptidomimetic research

• Enzyme inhibitor development

• Medicinal chemistry

• Peptide fragment coupling

• Modified peptide synthesis

• Bioconjugation intermediates

• Peptide library development

• Research-scale and batch synthesis

Peptide alcohols can serve as final research compounds or as intermediates for further chemical modification.

H-Thr-ol vs H-Thr(tBu)-ol Resin

These products are not interchangeable. The protection state should be confirmed before ordering or starting synthesis.

Comparison with Other SPPS Resins

Choose H-Thr-ol-2-CT Resin when a threoninol-derived C-terminal amino alcohol is required.

Loading Selection and Quality Control

Lower loading may improve reagent accessibility for long, hydrophobic or aggregation-prone sequences. Higher loading may increase productivity for shorter peptides.

Recommended qualification parameters include:

• Threoninol loading

• Building-block identity

• Stereochemical designation

• Amino-group availability

• Hydroxyl protection state

• Particle-size distribution

• DVB crosslinking percentage

• Moisture content

• Swelling performance

• Test-coupling efficiency

• Mild-cleavage performance

• Lot-to-lot consistency

Keep the resin tightly sealed in a cool, dry environment according to the product label and SDS.

Frequently Asked Questions

What is H-Thr-ol-2-CT Resin?

It is a 2-chlorotrityl polystyrene resin preloaded with threoninol for the solid-phase synthesis of C-terminal peptide alcohols.

What does “H” mean?

“H” indicates that the threoninol amino group is unprotected and available for direct amino acid coupling.

What does “ol” mean?

“ol” indicates that the loaded building block is threoninol, the amino-alcohol derivative of threonine.

Is this the same as H-Thr(tBu)-ol-2-CT Resin?

No. H-Thr(tBu)-ol contains a tert-butyl-protected hydroxyl group. The protection state must be confirmed before use.

Is the resin compatible with Fmoc SPPS?

Yes. The free threoninol amine can be coupled with an Fmoc-protected amino acid, followed by standard Fmoc deprotection and coupling cycles.

What product is obtained after cleavage?

The resin is designed to release a peptide containing a C-terminal threoninol or amino-al