100nm Carboxyl Red-Fluo Microspheres CFR100-10 Overview

CFR100-10 100nm Carboxyl Red-Fluo Microspheres are high-performance fluorescent polymer microspheres developed for lateral flow immunoassay (LFIA), fluorescent immunoassay research, antibody labeling, and diagnostic reagent manufacturing.

With a controlled particle size of 100nm, red fluorescent signal, carboxyl-functionalized surface, and 1% solids concentration, CFR100-10 provides a reliable fluorescent labeling material for biotechnology companies, IVD reagent developers, and research organizations.

The surface carboxyl groups allow efficient covalent coupling with antibodies, proteins, peptides, antigens, and other biomolecules through EDC/NHS chemistry.

CFR100-10 is suitable for laboratory research, assay optimization, pilot-scale validation, and industrial bulk production of fluorescent immunochromatography reagents.

Product Specifications of CFR100-10 Red Fluorescent Microspheres

Key Features of 100nm Carboxyl Red-Fluo Microspheres

100nm Particle Size for Flexible Immunochromatography Development

CFR100-10 features a precisely controlled particle diameter of approximately 100nm.

Compared with smaller nanoparticles, 100nm fluorescent microspheres provide:

• Higher fluorescent loading capacity

• Strong optical signal output

• Stable conjugate formation

• Good membrane migration performance

• Flexible assay optimization capability

The 100nm particle size offers a balance between fluorescence intensity, surface area, and biological conjugation efficiency.

This makes CFR100-10 suitable for companies developing fluorescent lateral flow and immunoassay platforms.

Bright Red Fluorescence for Quantitative Detection

CFR100-10 incorporates red fluorescent dyes within the polymer matrix to provide stable optical performance.

The fluorescent signal supports:

• Quantitative immunochromatography

• Fluorescence lateral flow assays

• Reader-based detection systems

• Digital diagnostic platforms

• Multiplex assay development

Compared with traditional visible labels, fluorescent microspheres enable objective signal measurement and improved data analysis.

Carboxyl Functional Surface for Biomolecule Coupling

CFR100-10 contains surface carboxyl groups (-COOH), providing reactive sites for biomolecule immobilization.

Using EDC/NHS activation chemistry, CFR100-10 can be coupled with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Antigens

• Peptides

• Enzymes

• Nucleic acid probes

The covalent coupling approach improves conjugate stability and supports reproducible reagent preparation.

Excellent Dispersion Stability and Batch Consistency

Consistent particle properties are essential for commercial diagnostic reagent development.

CFR100-10 provides:

• Uniform particle size distribution

• Stable aqueous dispersion

• Reliable conjugation performance

• Good batch reproducibility

• Scalable manufacturing capability

These characteristics help customers move from laboratory research to industrial production.

Applications of CFR100-10 in Lateral Flow Immunoassays

Fluorescent Lateral Flow Assay Development

CFR100-10 is designed for fluorescent lateral flow assay research and development.

Applications include:

• Infectious disease testing

• Biomarker detection

• Food safety analysis

• Veterinary diagnostics

• Environmental monitoring

• Pharmaceutical testing

The red fluorescence signal can be measured using fluorescence readers for quantitative analysis.

Antibody and Protein Conjugation

Carboxyl fluorescent microspheres provide an effective platform for antibody labeling.

Typical conjugation targets include:

• Detection antibodies

• Capture antibodies

• Antigens

• Binding proteins

• Peptides

• Other biological molecules

Researchers can optimize coupling density according to assay requirements.

Quantitative Immunochromatography Platforms

Fluorescent LFIA systems provide advantages including:

• Digital signal output

• Reduced subjective interpretation

• Reader compatibility

• Quantitative detection capability

CFR100-10 supports companies developing next-generation rapid diagnostic products.

Diagnostic Reagent Production

CFR100-10 supports the complete reagent development workflow:

• Raw material screening

• Fluorescent conjugate preparation

• Strip optimization

• Pilot testing

• Production transfer

• Bulk manufacturing

How Do 100nm Fluorescent Microspheres Improve LFIA Performance?

In fluorescent lateral flow assays:

1. CFR100-10 microspheres are conjugated with detection antibodies.

2. Samples are applied onto the test strip.

3. Target molecules bind with fluorescent conjugates.

4. Complexes migrate through the membrane.

5. Target molecules are captured at the test line.

6. Fluorescence intensity is measured by an optical reader.

7. Detection results are calculated based on fluorescence signals.

The final assay performance depends on:

• Antibody affinity

• Coupling efficiency

• Particle concentration

• Membrane selection

• Buffer formulation

• Detection system

Antibody Coupling Process Using Carboxyl Red-Fluo Microspheres

Step 1: Carboxyl Activation

Carboxyl groups on CFR100-10 are activated using EDC or EDC/NHS chemistry.

Key factors include:

• Buffer pH

• Activator concentration

• Reaction time

• Particle concentration

Step 2: Antibody Conjugation

Activated microspheres react with amino groups on antibodies or proteins.

Optimization parameters:

• Antibody concentration

• Particle/protein ratio

• Reaction temperature

• Incubation time

Step 3: Blocking and Purification

After conjugation:

• Remaining active sites are blocked

• Unbound proteins are removed

• Fluorescent conjugates are purified

Step 4: Performance Evaluation

Quality evaluation includes:

• Particle size analysis

• Fluorescence intensity

• Coupling efficiency

• Dispersion stability

• LFIA strip performance

Advantages of Fluorescent Microspheres Compared with Colloidal Gold

Quality Control and Bulk Manufacturing Capability

For diagnostic reagent manufacturers, consistent raw materials are critical.

CFR100-10 quality control includes:

Particle Size Control

Ensures consistent migration and conjugation.

Fluorescence Testing

Evaluates signal intensity and stability.

Functional Group Analysis

Confirms available carboxyl groups.

Solid Content Verification

Maintains formulation consistency.

Batch Consistency Evaluation

Supports reliable commercial production.

Customization Options for CFR100-10

SHBC provides customized fluorescent microsphere solutions.

Particle Size Customization

Available particle sizes can be optimized for:

• Membrane migration

• Detection sensitivity

• Assay design

Fluorescence Customization

Options include:

• Different fluorescent colors

• Customized emission properties

• Reader compatibility optimization

Surface Functionalization

Available modifications:

• Carboxyl

• Amino

• Streptavidin

• Other functional groups

Concentration Customization

Solid content can be adjusted according to:

• Coupling requirements

• Shipping conditions

• Production process

OEM and Bulk Production

SHBC supports:

• Research samples

• Pilot production

• Bulk supply

• Custom packaging

• Long-term supply agreements

Frequently Asked Questions About CFR100-10

What are 100nm Carboxyl Red-Fluo Microspheres?

CFR100-10 are 100nm fluorescent polymer microspheres with carboxyl functional surfaces designed for LFIA, immunoassay development, and biomolecule coupling.

Can CFR100-10 be used for immunochromatography?

Yes. CFR100-10 is suitable for fluorescent lateral flow assay research and diagnostic reagent development.

How are antibodies attached to CFR100-10?

Antibodies are commonly coupled using EDC/NHS chemistry, creating stable covalent bonds between microsphere carboxyl groups and antibody amino groups.

Why choose 100nm fluorescent microspheres?

100nm microspheres provide strong fluorescence output, good conjugation efficiency, and stable performance for immunoassay development.

Can CFR100-10 be supplied in bulk quantities?

Yes. SHBC provides laboratory samples, pilot batches, and industrial bulk production.

Are customized specifications available?

Yes. Particle size, fluorescence properties, surface chemistry, concentration, and packaging can be customized.

Why Choose SHBC CFR100-10 Carboxyl Red-Fluo Microspheres?

SHBC specializes in fluorescent microsphere materials for immunodiagnostic research and reagent manufacturing.

CFR100-10 provides:

• 100nm controlled particle size

• Red fluorescent labeling

• Carboxyl functional surface

• Stable biomolecule coupling

• LFIA development support

• Bulk production capability

• OEM customization service

Contact SHBC for technical specifications, samples, and bulk quotation for 100nm Carboxyl Red-Fluo Microspheres CFR100-10.