100nm Polystyrene Microspheres PS100-10 5%

100nm Polystyrene Microspheres PS100-10

SHBC PS100-10 Polystyrene Microspheres are uniform 100nm latex particles supplied at 5% solids.

The standard polystyrene surface supports the physical adsorption of antibodies, antigens and other proteins, making the particles suitable for immunoassay research and reagent manufacturing.

Product Overview

PS100-10 provides a stable particle carrier for adsorption-based biomolecule immobilization.

Its 100nm particle size offers a high specific surface area for protein coating, while the 5% solids concentration supports laboratory evaluation, formulation development and bulk reagent production.

No chemical activation is normally required for initial physical adsorption studies.

Key Specifications

Advantages for Physical Adsorption

• Uniform 100nm particle size

• High specific surface area

• Standard hydrophobic polystyrene surface

• Suitable for passive protein adsorption

• No EDC or NHS activation required

• Stable aqueous dispersion

• Easy dilution and formulation

• Consistent batch production

• Suitable for pilot and bulk manufacturing

• Custom concentration and packaging available

Immunoassay Applications

PS100-10 Polystyrene Microspheres can be evaluated for:

• Antibody adsorption

• Antigen immobilization

• Protein coating studies

• Latex-enhanced immunoassays

• Agglutination assay development

• Immunoturbidimetric reagent research

• Diagnostic reagent formulation

• Blocking buffer screening

• Non-specific adsorption studies

• Biomolecule–particle interaction research

The final performance depends on the protein, coating concentration, buffer, pH, ionic strength and blocking system.

Physical Adsorption Principle

Proteins can adsorb onto the polystyrene surface through hydrophobic interactions, ionic interactions and other non-covalent forces.

The adsorption process is simple and does not require chemical coupling reagents. It is suitable for rapid antibody or antigen coating screening during early assay development.

Physical adsorption is less orientation-controlled than covalent coupling. Coating and blocking conditions should therefore be optimized for each biomolecule.

Recommended Coating Development

A typical development process includes:

1. Disperse the microspheres completely.

2. Dilute to the required working concentration.

3. Add the antibody, antigen or protein.

4. Incubate under controlled mixing conditions.

5. Separate or wash away unbound protein.

6. Add a suitable blocking reagent.

7. Resuspend in the storage buffer.

8. Evaluate sensitivity, stability and non-specific binding.

Protein concentration, pH, coating time and blocking formulation should be determined experimentally.

Why Choose 100nm Polystyrene Microspheres

The 100nm particle size provides a large surface area for biomolecule adsorption and is suitable for nanoscale immunoassay reagent development.

Compared with larger particles, 100nm microspheres provide more particles and more available coating surface at the same solids mass.

PS100-10 is suitable for customers requiring concentrated polystyrene latex raw material for formulation screening and scalable production.

Why Choose SHBC

Shanghai SanYu Biotechnology Co., Ltd. specializes in microsphere materials for immunoassay and life science research.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle dispersion

• Batch-to-batch consistency

• Laboratory and bulk quantities

• Technical support for coating development

• Flexible packaging options

• OEM and private-label services

• Customized surface chemistry

Bulk Supply and Customization

PS100-10 is available for research evaluation, pilot production and bulk manufacturing.

Customization options include:

• Particle size

• Solids concentration

• Buffer formulation

• Surfactant level

• Preservative system

• Surface functional groups

• Bottle volume

• Bulk packaging

• OEM labeling

Carboxyl, amino and other functionalized polystyrene microspheres are also available for covalent biomolecule coupling.

Frequently Asked Questions

What is PS100-10?

PS100-10 is a 100nm polystyrene microsphere suspension supplied at 5% solids.

Can antibodies be adsorbed directly?

Yes. The standard polystyrene surface can be used for passive antibody and protein adsorption without chemical activation.

Is EDC/NHS activation required?

No. EDC/NHS activation is not normally required for physical adsorption onto standard polystyrene microspheres.

What proteins can be coated?

The particles can be evaluated for antibodies, antigens, enzymes and other proteins. Adsorption efficiency must be tested for each biomolecule.

Can PS100-10 be used for immunoturbidimetric assays?

It can be evaluated as a particle carrier for latex-enhanced immunoturbidimetric reagent development. The formulation and reaction conditions require application-specific optimization.

What affects physical adsorption?

Important factors include protein concentration, buffer pH, ionic strength, particle concentration, coating time, temperature and blocking reagent.

Is bulk production available?

Yes. SHBC supports sample testing, pilot-scale production and bulk supply for research institutions and immunoassay reagent manufacturers.

Can the concentration be customized?

Yes. Solids concentration, buffer, packaging and other specifications can be customized according to project requirements.

Research Use Only

PS100-10 Polystyrene Microspheres are intended for research use and reagent development only. They are not intended for direct clinical diagnosis or therapeutic use.