100um Carboxyl Red-Fluo Microspheres CFR100UM-10 1%

100um Carboxyl Red-Fluo Microspheres CFR100UM-10

SHBC CFR100UM-10 are 100 μm carboxyl-functionalized red fluorescent microspheres developed for fluorescence imaging, particle tracing, fluid-flow research and biomolecule immobilization.

The microspheres feature excitation at 535 nm, emission at 610 nm and 1% solid content. Surface carboxyl groups support covalent coupling with proteins, antibodies, peptides and other amino-containing molecules.

Product Specifications

Batch-specific particle size, size distribution, fluorescence intensity and carboxyl content are available in the Certificate of Analysis.

Red Fluorescence at 535/610nm

CFR100UM-10 produces red fluorescence with excitation at approximately 535 nm and emission centered near 610 nm.

The large fluorescent particles can be observed using compatible fluorescence microscopes, imaging systems and optical readers.

Key Advantages

Large 100um Particle Size

The large particle diameter supports easy optical identification, imaging and particle-distribution analysis.

Clear Red Fluorescence

The 535/610 nm fluorescence profile provides a clear red signal for tracing and imaging applications.

Reactive Carboxyl Surface

Surface COOH groups support covalent immobilization of antibodies, proteins, peptides and other amino-containing molecules.

Large Coupling Area per Particle

Each 100 μm particle provides substantial surface area for ligand immobilization and surface modification.

Easy Separation and Recovery

The large particles can be conveniently collected by sedimentation, filtration or low-speed centrifugation.

Custom and Bulk Supply

SHBC supports laboratory samples, customized specifications, pilot production and bulk manufacturing.

Main Applications

Fluorescence Microscopy

CFR100UM-10 can be used for particle localization, distribution analysis and fluorescence imaging.

Fluid and Flow Tracing

Suitable for selected flow visualization, liquid-distribution and particle-transport studies.

Filtration Research

Can be used to evaluate particle retention, filter performance, pore passage and separation processes.

Microfluidic Testing

Suitable for large-channel visualization, particle transport and microfluidic device evaluation.

Solid-Phase Assays

The carboxyl surface can be modified with antibodies, antigens or proteins for selected solid-phase assay formats.

Biomolecule Immobilization

Suitable for coupling proteins, peptides, enzymes and other molecules containing primary amine groups.

Surface Interaction Studies

Can be used to study particle adhesion, surface adsorption and particle–material interactions.

Optical Method Development

Suitable for fluorescence imaging, particle counting and optical detection method development.

Biomolecule Coupling

Surface carboxyl groups can be activated using EDC or EDC/NHS chemistry and reacted with primary amine groups on proteins.

A general workflow includes:

1. Wash and redisperse the microspheres.

2. Activate the surface carboxyl groups.

3. Add the target protein or ligand.

4. Block remaining reactive sites.

5. Wash and resuspend the conjugated particles.

6. Evaluate coupling and fluorescence performance.

Coupling pH, reagent ratio, protein loading and reaction time should be optimized for each biomolecule.

Product Benefits

CFR100UM-10 provides:

• Large and easily observed particles

• Clear red fluorescence

• Reactive carboxyl surface

• Large coupling area per particle

• Easy washing and recovery

• Flexible surface modification

• Stable particle identification

• Custom manufacturing support

Imaging and Tracing Considerations

Recommended parameters to evaluate include:

• Particle concentration

• Excitation filter

• Emission filter

• Exposure time

• Objective magnification

• Background fluorescence

• Particle settling

• Flow velocity

• Surface adsorption

• Image-analysis settings

Use an optical configuration compatible with excitation near 535 nm and emission near 610 nm.

Dispersion and Handling

Before use:

• Mix by gentle inversion

• Redisperse settled particles completely

• Avoid excessive bubbles and foam

• Protect the suspension from strong light

• Prevent the microspheres from drying

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

• Use clean laboratory equipment

The large particles may settle rapidly. Mix the suspension immediately before sampling.

Separation and Washing

CFR100UM-10 can be recovered using:

• Low-speed centrifugation

• Sedimentation

• Filtration

• Sieving

• Manual particle collection

Select the method according to the sample volume, buffer viscosity and required recovery rate.

Avoid excessive centrifugation that may produce a compact pellet.

Application Limitations

The 100 μm particles are generally unsuitable for conventional nitrocellulose lateral flow assays.

They may also be too large for many standard flow cytometers, narrow microfluidic channels and cellular uptake studies.

Confirm instrument fluidics, channel dimensions, filter pore size and application compatibility before use.

Custom Carboxyl Fluorescent Microspheres

SHBC can customize fluorescent microspheres according to research and production requirements.

Customization options may include:

• Custom particle size

• Custom fluorescence wavelength

• Different fluorescence colors

• Carboxyl group density

• Solid content

• Dispersion medium

• Surface modification

• Biomolecule conjugation

• Custom packaging

• OEM and ODM production

Please provide the target application, required wavelength, surface ligand and order quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Microscopy images

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFR100UM-10?

CFR100UM-10 is a 100 μm carboxyl-functionalized red fluorescent microsphere suspension with 1% solid content.

What are its excitation and emission wavelengths?

The excitation wavelength is 535 nm and the emission wavelength is 610 nm.

What is CFR100UM-10 mainly used for?

It is suitable for fluorescence microscopy, fluid tracing, filtration research, particle tracking, surface studies and biomolecule coupling.

Can proteins be coupled to CFR100UM-10?

Yes. Proteins and other molecules containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling chemistry can be used?

EDC or EDC/NHS chemistry can be evaluated for activating the surface carboxyl groups.

Will CFR100UM-10 settle during storage or use?

The large particles may settle rapidly. Redisperse the suspension immediately before sampling or application.

Can CFR100UM-10 be separated by centrifugation?

Yes. Low-speed centrifugation, sedimentation or filtration can be evaluated for particle recovery.

Is CFR100UM-10 suitable for flow cytometry?

Many standard flow cytometers cannot process 100 μm particles. Instrument fluidics and flow-cell dimensions must be confirmed.

Is CFR100UM-10 suitable for lateral flow assays?

No. The particles are generally too large for conventional nitrocellulose membrane migration.

Can CFR100UM-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, dispersion medium and packaging can be customized.

Are samples and bulk quantities available?

Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.

Request a Sample or Quotation

Contact SHBC for CFR100UM-10 samples, technical specifications, conjugation support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Detection equipment

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 100um Carboxyl Red-Fluo Microspheres
Catalog Number: CFR100UM-10
Particle Size: 100 μm
Solid Content: 1%
Excitation/Emission: 535/610 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.