10um Carboxy Silica Microspheres CSi10UM-10 5%

10um Carboxyl Silica Microspheres for Immunoassay R&D

SHBC CSi10UM-10 Carboxyl Silica Microspheres are 10 µm silica particles with surface carboxyl groups and a solid content of 5%.

The carboxyl-functionalized surface supports covalent immobilization of antibodies, antigens, proteins, peptides and other biomolecules. The product is suitable for immunoassay research, solid-phase carrier development and diagnostic reagent production.

Product Specifications

Batch-specific particle size, dispersion medium and surface carboxyl content should be confirmed in the applicable product specification.

Key Features and Advantages

Reactive Carboxyl Surface

Surface carboxyl groups can be activated for covalent coupling with amino groups on antibodies, proteins, peptides and other biomolecules.

Practical 10um Particle Size

The 10 µm particle size provides a useful balance between available coupling surface, particle recovery and handling during assay development.

Convenient 5% Suspension

The standard 5% solid content supports laboratory dilution, coupling optimization, pilot production and batch formulation.

Stable Silica Matrix

The silica structure provides a stable solid-phase carrier for repeated washing, surface modification and biomolecule immobilization.

Easy Particle Recovery

The microspheres can be evaluated for separation by centrifugation, filtration or sedimentation, depending on the assay format.

Scalable Supply

SHBC supports research samples, customized specifications, pilot orders and bulk manufacturing.

Carboxyl Surface for Biomolecule Coupling

CSi10UM-10 can be evaluated for immobilizing:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Amino-modified oligonucleotides

• Affinity ligands

• Other amine-containing biomolecules

Carboxyl-functionalized silica microspheres are commonly coupled to amino-containing biomolecules using carbodiimide chemistry. EDC, often combined with NHS or Sulfo-NHS, activates the surface carboxyl groups before reaction with primary amino groups.

The final coupling efficiency depends on buffer pH, protein concentration, reagent ratio, reaction time and blocking conditions.

Immunoassay Applications

CSi10UM-10 can be evaluated for:

• Antibody immobilization

• Antigen coating

• Solid-phase immunoassays

• Particle-capture ELISA

• Immunofluorescence assay development

• Agglutination assay research

• Biomolecule conjugation

• Affinity capture

• Biosensor carrier development

• Protein binding studies

• Nucleic acid probe immobilization

• Multiplex detection research

• Diagnostic reagent raw material development

Functionalized silica microspheres have been used as solid carriers in bead-based immunoassays and microfluidic detection systems.

Why Choose 10um Carboxyl Silica Microspheres?

Compared with nanoscale particles, 10 µm silica microspheres are easier to recover and wash during laboratory development.

Key benefits include:

• Convenient centrifugation and filtration

• Easy microscopic observation

• Stable solid-phase carrier

• Flexible biomolecule immobilization

• Suitable for repeated washing

• Reduced particle loss during manual operation

• Compatible with different coupling strategies

• Suitable for research-to-production scale-up

Customers should confirm compatibility with their analyzer, dispensing system and assay format before commercial production.

Recommended EDC/NHS Coupling Workflow

A typical development workflow may include:

1. Resuspend and wash the microspheres.

2. Prepare the microspheres in an amine-free activation buffer.

3. Add freshly prepared EDC and NHS or Sulfo-NHS.

4. Activate the surface carboxyl groups.

5. Wash or adjust the reaction conditions.

6. Add the antibody, antigen or target protein.

7. Incubate under gentle mixing.

8. Block remaining active sites.

9. Wash the conjugated microspheres.

10. Resuspend in a suitable storage buffer.

The reagent concentration, reaction time and protein loading should be optimized for each biomolecule.

Assay Optimization Considerations

Important development parameters include:

• Microsphere-to-protein ratio

• EDC and NHS concentration

• Activation pH

• Coupling pH

• Protein concentration

• Reaction time

• Mixing method

• Blocking reagent

• Washing conditions

• Storage buffer

• Preservative compatibility

• Nonspecific adsorption

Excessive activation may increase particle aggregation or reduce biomolecule activity. Small-scale screening is recommended before production scale-up.

Recommended Handling

• Mix gently before sampling.

• Resuspend settled microspheres completely.

• Avoid allowing the particles to dry.

• Use clean, low-binding containers.

• Avoid buffers containing free primary amines during EDC/NHS activation.

• Use gentle mixing to reduce aggregation.

• Optimize centrifugation conditions before scale-up.

• Avoid repeated freeze-thaw cycles.

• Follow the batch-specific storage instructions.

Customization and Bulk Production

Shanghai SanYu Biotechnology Co., Ltd. provides carboxyl silica microspheres for biotechnology companies, research institutions and diagnostic reagent manufacturers.

Customization options may include:

• Different particle sizes

• Different solid contents

• Carboxyl group density adjustment

• Custom dispersion media

• Fluorescent modification

• Protein or antibody conjugation

• Custom packaging

• Pilot-scale production

• Bulk manufacturing

• OEM and ODM services

Customization feasibility depends on the required specification, application and order volume.

Why Choose SHBC?

• Specialized microsphere manufacturer

• Controlled 10 µm particle size

• Reactive carboxyl surface

• Standard 5% solid content

• Suitable for biomolecule coupling

• Support for immunoassay development

• Sample and pilot-order availability

• Stable batch production

• Customized surface chemistry

• Bulk supply capability

• Custom packaging

• OEM and ODM support

Quality Documentation

Available documentation may include:

• Certificate of Analysis

• Safety Data Sheet

• Product specification

• Particle size information

• Solid-content information

• Batch identification

• Storage guidance

• Handling recommendations

Please confirm the available test items and acceptance criteria before placing a bulk order.

Frequently Asked Questions

What is CSi10UM-10?

CSi10UM-10 is a 10 µm carboxyl-functionalized silica microsphere suspension with a solid content of 5%, supplied by SHBC.

What can be coupled to the carboxyl surface?

Antibodies, antigens, proteins, peptides and amino-modified oligonucleotides can be evaluated for covalent coupling.

Which coupling chemistry is recommended?

EDC/NHS or EDC/Sulfo-NHS chemistry can be evaluated to activate the surface carboxyl groups for reaction with primary amino groups.

Why choose 10um silica microspheres?

The 10 µm size supports convenient washing, centrifugation, filtration and microscopic observation during assay development.

Can the microspheres be used for immunoassays?

Yes. They can be evaluated for solid-phase immunoassays, particle-capture assays, affinity capture and biomolecule immobilization.

Is blocking required after coupling?

Blocking is normally evaluated to reduce remaining reactive sites and nonspecific binding.

Can SHBC customize the particle size?

Yes. Different particle sizes, concentrations, surface group densities and dispersion media can be discussed.

Are bulk quantities available?

Yes. SHBC supports sample evaluation, pilot orders and bulk production.

Is CSi10UM-10 a finished diagnostic reagent?

No. It is a raw material for research and reagent development. Customers must complete application-specific validation.

Request Samples and Bulk Pricing

Contact SHBC for CSi10UM-10 samples, technical specifications, customized surfaces and bulk quotations.

Please provide:

• Required quantity

• Target application

• Target biomolecule

• Preferred coupling method

• Packaging requirements

• Expected annual demand

Product: 10um Carboxyl Silica Microspheres
Catalog Number: CSi10UM-10
Particle Size: 10 µm
Solid Content: 5%
Surface Group: Carboxyl, –COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Intended Use: Research and Reagent Development Use