10um Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB10UM 10%

10 μm Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB10UM

SHBC CPS/DVB10UM Carboxyl Crosslinked PS/DVB Microspheres are 10 μm functional polymer microspheres with a crosslinked polystyrene/divinylbenzene matrix and surface carboxyl groups.

Supplied at 10% solids, CPS/DVB10UM is designed for antibody coupling, protein immobilization, bead-based immunoassays and scalable reagent development.

CPS/DVB10UM Technical Specifications

Crosslinked PS/DVB Structure

The microsphere matrix consists of polystyrene crosslinked with divinylbenzene.

DVB crosslinking provides a more rigid polymer network and is commonly used in larger polystyrene microspheres when improved structural stability is required.

Potential advantages include:

• Good particle rigidity

• Stable spherical morphology

• Improved structural stability

• Suitable for repeated washing

• Suitable for surface functionalization

• Reliable handling in bead-based assays

Carboxyl Surface for Covalent Coupling

Surface carboxyl groups provide reactive sites for covalent biomolecule immobilization.

Typical coupling chemistry:

COOH Surface → EDC/NHS Activation → Amine-Containing Biomolecule → Stable Amide Bond

Carboxyl-functional polystyrene microspheres are widely used for covalent immobilization of proteins, peptides and amino-modified nucleic acids through carbodiimide-mediated chemistry.

Key Advantages of CPS/DVB10UM

• 10 μm particle size

• High 10% solid content

• Crosslinked PS/DVB matrix

• Reactive carboxyl surface

• Suitable for EDC/NHS coupling

• Stable covalent ligand attachment

• Convenient particle washing

• Suitable for antibodies and antigens

• Good choice for bead-based assays

• Suitable for bulk reagent production

Why Choose 10 μm Microspheres?

The 10 μm particle format provides convenient handling for bead-based research and immunoassay development.

Potential advantages include:

• Easy centrifugation

• Convenient washing

• Straightforward resuspension

• Good compatibility with optical detection

• Suitable for flow-based bead analysis

• Easy laboratory handling

Commercial crosslinked carboxyl polystyrene particles are available in approximately the 8–13 μm range, supporting this particle-size class for functional bead applications.

Immunoassay Applications

Antibody-Coupled Microspheres

Capture antibodies can be immobilized on CPS/DVB10UM for:

• Bead-based immunoassays

• Antigen detection

• Biomarker detection

• Serological assay development

• Multiplex assay research

• Diagnostic reagent development

Covalent coupling can provide stronger ligand retention than simple passive adsorption during repeated washing and storage.

Antigen-Coupled Microspheres

Antigens can be immobilized for:

• Antibody detection

• Serology research

• Autoantibody assays

• Infectious-disease research

• Assay development

• Method validation

Flow Cytometry Applications

The 10 μm particle size can also be evaluated for bead-based flow cytometry research.

Potential applications include:

• Antibody-binding assays

• Antigen detection

• Multiplex assay development

• Biomolecule-binding studies

• Flow cytometer method development

• Fluorescence assay research

Bangs lists latex bead-based flow cytometric immunoassay among applications associated with carboxyl polystyrene microspheres.

Protein and Biomolecule Applications

CPS/DVB10UM can also be evaluated for:

• Protein immobilization

• Enzyme immobilization

• Peptide coupling

• Affinity ligand preparation

• Biosensor development

• Biomolecule capture

• Surface chemistry research

• Amino-modified oligonucleotide coupling

Suitable Coupling Molecules

The carboxyl-functional surface can be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Diagnostic antigens

• Peptides

• Enzymes

• Amino-modified DNA

• Amino-modified RNA

• Other amine-containing ligands

Final performance depends on ligand purity, concentration and coupling conditions.

General EDC/NHS Coupling Workflow

1. Resuspend the Microspheres

Mix the CPS/DVB10UM suspension thoroughly before sampling.

2. Wash the Microspheres

Wash the microspheres using a suitable activation buffer.

3. Activate Carboxyl Groups

Add EDC with or without NHS to activate the surface carboxyl groups.

4. Add the Biomolecule

Add the antibody, antigen, protein or other amine-containing ligand.

5. Perform Covalent Coupling

Allow the activated carboxyl groups to react with primary amines on the biomolecule.

6. Block Residual Reactive Sites

Use a suitable blocking or quenching reagent.

7. Wash and Resuspend

Remove unbound biomolecules and coupling reagents, then resuspend the functionalized microspheres in the selected assay or storage buffer.

EDC-mediated coupling is an established approach for attaching amine-containing biomolecules to carboxyl-functionalized polymer microspheres.

Factors Affecting Coupling Efficiency

Important optimization factors include:

• Microsphere concentration

• Ligand concentration

• EDC concentration

• NHS concentration

• Activation pH

• Coupling-buffer composition

• Reaction time

• Reaction temperature

• Mixing efficiency

• Blocking conditions

Small-scale optimization is recommended before production scale-up.

Crosslinked PS/DVB vs. Standard PS Microspheres

Crosslinked PS/DVB microspheres are preferred when both particle stability and reactive surface chemistry are required.

Covalent Coupling vs. Passive Adsorption

Quality Control for Bulk Production

For immunoassay reagent development, evaluate:

• Particle-size consistency

• Suspension uniformity

• Solid-content consistency

• Surface carboxyl functionality

• Coupling efficiency

• Ligand loading

• Nonspecific binding

• Signal-to-background ratio

• Assay sensitivity

• Storage stability

• Batch-to-batch consistency

Why Choose SHBC CPS/DVB10UM?

• 10 μm carboxyl crosslinked PS/DVB microspheres

• 10% solid-content suspension

• Functional COOH surface

• Suitable for EDC/NHS coupling

• Designed for antibody and protein immobilization

• Suitable for immunoassay research

• Samples available for evaluation

• Stable batch production

• Bulk manufacturing capability

• Support for research and production quantities

Storage and Handling

Recommended practices include:

• Keep the container tightly sealed.

• Mix thoroughly before sampling.

• Prevent microbial contamination.

• Avoid unnecessary freezing.

• Use clean pipettes and containers.

• Avoid conditions causing irreversible aggregation.

• Validate storage conditions after biomolecule coupling.

Frequently Asked Questions

What is CPS/DVB10UM?

CPS/DVB10UM is a 10 μm carboxyl-functional crosslinked PS/DVB microsphere product supplied at 10% solids.

What is PS/DVB?

PS/DVB refers to polystyrene crosslinked with divinylbenzene.

Why use a crosslinked structure?

The crosslinked polymer network provides improved particle rigidity and structural stability compared with conventional non-crosslinked PS particles.

What is the function of the carboxyl surface?

Surface carboxyl groups provide reactive sites for covalent attachment of antibodies, proteins and other amine-containing biomolecules.

Can antibodies be coupled to CPS/DVB10UM?

Yes. Antibodies can be immobilized using optimized EDC or EDC/NHS coupling chemistry.

Can CPS/DVB10UM be used for immunoassays?

Yes. It can be evaluated for bead-based antigen, antibody and biomarker detection.

Can it be used for flow cytometry?

Yes. The 10 μm particle size can be evaluated in suitable bead-based flow cytometry applications.

What is the solid content?

CPS/DVB10UM is supplied at 10% solids.

Can amino-modified oligonucleotides be coupled?

Yes. Amino-modified DNA or RNA can be evaluated for carbodiimide-mediated coupling to the carboxyl surface.

Is coupling optimization required?

Yes. EDC/NHS concentration, pH, ligand loading, reaction time and blocking conditions should be optimized for each project.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk supply for immunoassay developers, biotechnology companies and research organizations.

Request CPS/DVB10UM Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for CPS/DVB10UM samples, technical documentation and bulk quotations.

Please provide:

• Target antibody or protein

• Assay format

• Coupling chemistry

• Required microsphere quantity

• Target application

• Development stage

• Estimated annual demand

CPS/DVB10UM is supplied for research and process development. Final diagnostic applications should be validated by the customer.