10um Carboxyl Green-Fluo Microspheres CFG10UM-10

10um Carboxyl Green-Fluo Microspheres CFG10UM-10

SHBC CFG10UM-10 are 10um green fluorescent microspheres with carboxyl-functionalized surfaces.

With excitation at 470/488nm and emission at 525nm, the microspheres provide strong particle-level fluorescence for flow cytometry, fluorescence microscopy, cell interaction studies, particle tracking and biomolecule conjugation.

Product Specifications

Key Advantages

• Bright green fluorescence at 525nm

• Compatible with 470nm and 488nm excitation

• Reactive carboxyl surface

• Strong fluorescence per particle

• Easy microscopic observation

• Large surface area for biomolecule loading

• Clear particle identification in flow systems

• Suitable for research and batch production

Why Choose 10um Green Fluorescent Microspheres?

The 10um particle size provides excellent optical visibility and easy particle discrimination.

Key benefits include:

• Convenient particle counting

• Strong single-particle fluorescence

• Easy localization under microscopy

• Large coupling surface

• Clear flow cytometry gating

• Good suitability for cell–particle interaction studies

Carboxyl Surface for Biomolecule Coupling

Surface carboxyl groups can be activated for covalent coupling with primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amino-containing molecules

EDC/NHS chemistry may be used to form stable amide bonds between the microspheres and target biomolecules.

Main Applications

Flow Cytometry

The 10um size and green fluorescence support clear particle identification in suitable flow cytometry systems.

Typical uses include:

• Fluorescent particle counting

• Binding assay development

• Particle population analysis

• Fluorescence channel evaluation

• Flow cytometry method development

Fluorescence Microscopy

CFG10UM-10 can be easily visualized using compatible fluorescence microscopes.

It is suitable for:

• Particle localization

• Fluorescence imaging

• Particle movement analysis

• Surface binding studies

• Material distribution research

Cell Uptake and Phagocytosis Research

The microspheres can be evaluated for studying interactions between cells and micrometer-sized particles.

Potential applications include:

• Phagocytosis studies

• Cell–particle binding

• Immune cell research

• Particle uptake analysis

• Intracellular localization

• Cell surface marker research

Particle Tracking and Tracing

Bright green fluorescence allows particle movement and distribution to be monitored in suitable research systems.

Applications include:

• Fluid-flow visualization

• Filtration studies

• Particle transport research

• Microfluidic tracking

• Material distribution analysis

Particle-Based Immunoassays

Antibodies, antigens or proteins can be immobilized on the carboxyl surface to prepare fluorescent capture particles.

Potential assay formats include:

• Fluorescent binding assays

• Sandwich immunoassays

• Competitive assays

• Antibody detection

• Antigen detection

• Fluorescent agglutination research

Multiplex Suspension Assays

Different particle sizes, fluorescence levels or surface ligands can be used to create distinguishable microsphere populations for multiplex research.

Biosensors and Biochips

The functional surface supports ligand immobilization for biosensors, microarrays, biochips and microfluidic detection platforms.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling and fluorescence performance.

Coupling pH, reagent concentration, reaction time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix gently before use.

• Protect from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid repeated freezing and thawing.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Application Notes

For flow cytometry, optimize particle concentration, detector settings, fluorescence filters, flow rate and gating strategy.

For cell studies, optimize the particle-to-cell ratio, incubation time and washing conditions.

Because 10um microspheres are larger than particles commonly used in lateral flow assays, membrane migration must be validated before use in lateral flow systems.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Particle size

• Fluorescence wavelength

• Fluorescence intensity

• Carboxyl density

• Alternative surface groups

• Solids content

• Suspension buffer

• Packaging volume

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, bioseparation and biological research.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot and bulk production

Frequently Asked Questions

What is CFG10UM-10?

CFG10UM-10 is a 10um carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.

Is it compatible with a 488nm laser?

Yes. CFG10UM-10 can be excited using a suitable 488nm laser and detected through a compatible green fluorescence channel.

Can it be coupled with antibodies?

Yes. Surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is it suitable for flow cytometry?

Yes. The 10um particle size and green fluorescence support particle counting, binding assays and flow cytometry research.

Can it be used for phagocytosis studies?

Yes. It can be evaluated for phagocytosis, cell uptake and cell–particle interaction research.

Is it suitable for fluorescence microscopy?

Yes. Its large particle size and strong fluorescence support easy imaging, localization and tracking.

Can SHBC provide samples and bulk quantities?

Yes. SHBC supports research samples, pilot-scale evaluation and bulk manufacturing.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 10um Carboxyl Green-Fluo Microspheres
Catalog Number: CFG10UM-10
Particle Size: 10um
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.