10um Crosslinked PMMA Microspheres PM10UM-300A

10µm Porous Crosslinked PMMA Microspheres with 300Å Pores

SHBC PM10UM-300A is a porous crosslinked polymethyl methacrylate microsphere with a nominal particle size of 10µm and a pore size of 300Å.

The controlled porous structure, rigid polymer matrix and moderately polar methacrylate surface make it suitable for chromatography packing, biomolecule separation, adsorbent development and custom stationary-phase research.

Product Overview

PM10UM-300A is manufactured from a crosslinked PMMA-based polymer matrix.

The 10µm particle size provides a practical balance between separation efficiency, column pressure and preparative loading. The 300Å pores provide internal surface access for peptides, oligonucleotides, proteins and other biomolecules.

The product can be supplied as bulk porous microsphere media for analytical research, preparative purification and chromatography-material development.

Key Features and Benefits

• Nominal particle size of 10µm

• Controlled pore size of 300Å

• Porous crosslinked PMMA matrix

• Uniform spherical morphology

• Accessible internal pore structure

• Moderately polar methacrylate surface

• Alternative selectivity to PS/DVB media

• Suitable for chromatography column packing

• Suitable for biomolecule purification

• Compatible with further surface modification

• Laboratory and bulk quantities available

• Custom particle and pore sizes supported

Product Specifications

Particle-size distribution, pore volume, specific surface area, crosslinking degree and supply form should be confirmed in the SHBC technical data sheet.

Benefits of 10µm Particles and 300Å Pores

Balanced Column Performance

The 10µm particle size provides useful separation efficiency while generally producing less column resistance than smaller analytical particles.

Wide-Pore Accessibility

The 300Å pore structure allows relatively large biomolecules to access the internal particle surface.

Increased Interaction Area

The porous structure provides more accessible internal area than nonporous PMMA particles.

Suitable for Preparative Purification

The combination of 10µm particles and wide pores is suitable for preparative column packing and process-development research.

Stable Crosslinked Structure

Crosslinking helps the polymer particles maintain their spherical shape and porous structure during packing, washing and chromatographic operation.

Recommended Applications

Peptide Purification

Suitable for analytical and preparative separation of synthetic peptides, biological peptides and peptide-related impurities.

Oligonucleotide Separation

Can be evaluated for purification of DNA or RNA oligonucleotides under optimized chromatographic conditions.

Protein Purification

The 300Å pores provide internal access for proteins, protein fragments and selected larger biomolecules.

Natural Product Separation

Suitable for method development involving plant extracts, fermentation products and other complex natural mixtures.

Pharmaceutical Intermediate Purification

Can be used for separating active compounds, intermediates and process-related impurities.

Polymeric Reversed-Phase Chromatography

The methacrylate polymer matrix can provide different retention and selectivity from conventional silica or highly hydrophobic PS/DVB media.

Size Exclusion Media Development

The controlled porous structure can be evaluated as a base material for size exclusion chromatography after suitable surface treatment and method validation.

Solid-Phase Extraction

Can be developed as a porous polymer sorbent for sample cleanup, concentration and impurity removal.

Functional Chromatography Media

The PMMA matrix can be further modified to prepare ion exchange, affinity, hydrophobic interaction or mixed-mode separation media.

PMMA vs. PS/DVB Chromatography Media

PMMA media may be preferred when PS/DVB provides excessive hydrophobic retention or when alternative selectivity is required.

Actual chromatographic performance depends on the analyte, mobile phase, pore structure and surface chemistry.

Advantages of the PMMA Matrix

Alternative Chromatographic Selectivity

The ester-containing methacrylate structure provides different surface polarity from aromatic PS/DVB materials.

Reduced Excessive Hydrophobic Retention

The PMMA matrix can provide milder interaction for selected proteins, peptides and polar analytes.

Flexible Surface Modification

The polymer surface can be activated or coated with functional groups and application-specific ligands.

No Silanol Surface

PMMA-based particles do not contain the residual silanol groups found on conventional silica materials.

Suitable for Custom Resin Development

The porous matrix can serve as a base particle for developing specialized chromatography and adsorption media.

Chromatography Packing Applications

PM10UM-300A can be evaluated for packing:

• Analytical chromatography columns

• Semi-preparative columns

• Preparative purification columns

• Peptide purification columns

• Oligonucleotide purification columns

• Protein separation columns

• Solid-phase extraction cartridges

• Custom research columns

• Functionalized chromatography resins

This product is supplied as bulk microsphere media unless a prepacked column is specifically ordered.

General Column Packing Procedure

1. Select a compatible slurry liquid.

2. Fully wet and disperse the PMMA microspheres.

3. Prepare a uniform slurry without visible aggregates.

4. Remove trapped air when necessary.

5. Transfer the slurry into the column or packing reservoir.

6. Pack using controlled flow or pressure.

7. Avoid exceeding the validated pressure limit.

8. Flush the packed bed with the working mobile phase.

9. Equilibrate until pressure and baseline are stable.

10. Test column efficiency with a suitable standard.

Packing pressure, slurry concentration and solvent should be optimized for the selected column hardware.

Method Development Considerations

Chromatographic performance may be influenced by:

• Analyte molecular size

• Analyte polarity

• Mobile-phase composition

• Organic solvent concentration

• Buffer pH

• Ionic strength

• Column temperature

• Sample loading

• Flow rate

• Surface functionalization

Small-scale screening is recommended before preparative or industrial scale-up.

Surface Functionalization Options

SHBC can provide customized porous PMMA microspheres with different surface properties.

Available options may include:

• Carboxyl groups

• Amino groups

• Hydroxyl groups

• Epoxy groups

• Quaternary ammonium groups

• Sulfonic acid groups

• Hydrophobic coatings

• Hydrophilic coatings

• Affinity ligands

• Mixed-mode functional groups

• Custom molecules

Functionalized products can be developed for ion exchange, affinity purification, hydrophobic interaction, enzyme immobilization and specialized adsorption.

Why Choose SHBC

Controlled Particle Manufacturing

SHBC applies controlled polymerization, pore formation and particle-classification processes.

Defined Particle and Pore Size

PM10UM-300A combines a 10µm particle size with a controlled 300Å porous structure.

Flexible Product Customization

Particle size, pore size, crosslinking degree, surface chemistry and packaging can be customized.

Reliable Batch Consistency

Controlled production and quality inspection support consistent product performance between batches.

Scale-Up Manufacturing

SHBC supports laboratory evaluation, pilot-scale purification and bulk production.

Technical Support

Technical assistance is available for media selection, slurry preparation, column packing and surface modification.

Custom and Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supplies porous PMMA microspheres to pharmaceutical companies, biotechnology manufacturers, chromatography developers and research institutions.

Available customization includes:

• Custom particle size

• Custom pore size

• Custom pore volume

• Custom specific surface area

• Custom crosslinking degree

• Surface functionalization

• Custom supply form

• Custom packaging

• Pilot-scale production

• Bulk manufacturing

• OEM and ODM services

• Batch quality documentation

Please provide the target analyte, separation mode, column dimensions and estimated order quantity when requesting a quotation.

Frequently Asked Questions

What is PM10UM-300A?

PM10UM-300A is a porous crosslinked PMMA microsphere with a nominal particle size of 10µm and a pore size of 300Å.

What does 300Å mean?

It represents a nominal pore diameter of approximately 30nm inside the microspheres.

What is the benefit of 300Å pores?

The wide pores provide internal access for peptides, oligonucleotides, proteins and other relatively large analytes.

What is the advantage of 10µm particles?

The 10µm size provides a balance between chromatographic efficiency, loading capacity and operating pressure.

Is PM10UM-300A suitable for protein purification?

Yes. It can be evaluated for protein and protein-fragment separation when the pore structure and surface chemistry match the target molecule.

Can it be used for reversed-phase chromatography?

Yes. Porous methacrylate polymer media can be used for polymeric reversed-phase chromatography under optimized conditions.

How is PMMA different from PS/DVB?

PMMA has a more polar methacrylate surface, while PS/DVB generally provides stronger hydrophobic retention.

Can it be used for ion exchange chromatography?

The standard material does not contain ion-exchange groups. Charged surface groups can be customized.

Is this a finished chromatography column?

No. PM10UM-300A is supplied as bulk packing media unless a prepacked column is specifically requested.

Can SHBC customize the pore size?

Yes. Particle size, pore size, crosslinking degree and surface chemistry can be customized.

Is bulk production available?

Yes. SHBC supports samples, pilot-scale quantities and bulk manufacturing.

Storage and Handling

Store PM10UM-300A according to the SHBC technical documentation.

Keep the product tightly sealed and protected from contamination. Avoid exposure to unvalidated solvents, temperatures, pressures and strong oxidizing agents.

Request a Sample or Quotation

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized specifications or bulk pricing.

Product: 10µm Porous Crosslinked PMMA Microspheres
Catalog Number: PM10UM-300A
Brand: SHBC
Particle Size: 10µm
Pore Size: 300Å
Matrix: Crosslinked PMMA
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

For research and industrial chromatography use only.