60 um Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB60UM 10%

60 μm Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB60UM

SHBC CPS/DVB60UM Carboxyl Crosslinked PS/DVB Microspheres are 60 μm functional polymer microspheres with a crosslinked polystyrene/divinylbenzene matrix and reactive surface carboxyl groups.

Supplied at 10% solids, CPS/DVB60UM is designed for antibody coupling, protein immobilization, bead-based immunoassay development and custom functional particle production.

The larger particle format provides convenient washing, recovery and handling for research and scalable production.

CPS/DVB60UM Technical Specifications

Crosslinked PS/DVB Structure

CPS/DVB60UM uses divinylbenzene to crosslink the polystyrene matrix.

DVB crosslinking creates a more rigid polymer structure suitable for larger microspheres and repeated processing. Commercial PS microspheres of larger sizes are commonly crosslinked with DVB to maintain particle structure.

Key benefits include:

• Stable spherical morphology

• Good particle rigidity

• Convenient repeated washing

• Reliable particle recovery

• Suitable surface functionalization

• Easy handling in batch processes

Carboxyl Surface for Covalent Coupling

Surface carboxyl groups provide reactive sites for covalent attachment of amine-containing biomolecules.

Typical coupling route:

COOH Surface → EDC/NHS Activation → Amine-Containing Ligand → Stable Amide Bond

Carboxyl-functional PS and PS/DVB microspheres are commonly used for EDAC/EDC-mediated immobilization of proteins, peptides, DNA and other amine-containing molecules.

Key Advantages of CPS/DVB60UM

• 60 μm particle size

• High 10% solid content

• Crosslinked PS/DVB matrix

• Reactive carboxyl surface

• Suitable for EDC/NHS coupling

• Stable covalent ligand attachment

• Easy particle recovery

• Convenient washing and centrifugation

• Suitable for antibodies and antigens

• Suitable for bulk production

Why Choose 60 μm Carboxyl Microspheres?

The 60 μm particle format is suitable for applications where straightforward particle handling and recovery are important.

Potential advantages include:

• Fast sedimentation

• Easy centrifugation

• Convenient washing

• Easy visual observation

• Reduced particle loss during processing

• Suitable for batch coupling

• Straightforward resuspension

• Convenient scale-up

Larger microspheres generally provide easier physical handling, while smaller particles provide greater total surface area per unit mass.

Immunoassay Applications

Antibody-Coupled Microspheres

Capture antibodies can be covalently immobilized on CPS/DVB60UM for:

• Bead-based immunoassays

• Antigen detection

• Biomarker detection

• Serological assay development

• Pathogen-detection research

• Custom diagnostic reagent development

Covalent immobilization is useful when stable antibody retention is required during washing and storage.

Antigen-Coupled Microspheres

Antigens can be immobilized for:

• Antibody detection

• Serology research

• Autoantibody assay development

• Infectious-disease research

• Assay development

• Method validation

Protein and Biomolecule Applications

CPS/DVB60UM can also be evaluated for:

• Protein immobilization

• Enzyme immobilization

• Peptide coupling

• Affinity ligand preparation

• Biomolecule capture

• Biosensor development

• Surface chemistry research

• Amino-modified oligonucleotide coupling

• Custom functional particle preparation

Suitable Coupling Molecules

The carboxyl-functional surface can be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Diagnostic antigens

• Peptides

• Enzymes

• Amino-modified DNA

• Amino-modified RNA

• Other amine-containing ligands

Final coupling efficiency depends on ligand purity, concentration and reaction conditions.

General EDC/NHS Coupling Workflow

1. Resuspend the Microspheres

Mix CPS/DVB60UM thoroughly before sampling.

Because 60 μm particles settle relatively quickly, resuspend the particles immediately before use.

2. Wash the Microspheres

Wash the particles using a suitable activation buffer.

3. Activate Carboxyl Groups

Add EDC, with or without NHS, to activate the surface carboxyl groups.

4. Add the Biomolecule

Add the purified antibody, antigen, protein or other amine-containing ligand.

5. Perform Covalent Coupling

Allow activated carboxyl groups to react with primary amino groups on the biomolecule.

6. Block Residual Reactive Sites

Use a suitable blocking or quenching reagent.

7. Wash and Resuspend

Remove unbound biomolecules and coupling reagents, then resuspend the functionalized microspheres in the selected assay or storage buffer.

EDC-mediated coupling is an established method for covalent immobilization on carboxyl-functionalized polymer microspheres.

Factors Affecting Coupling Efficiency

Important optimization parameters include:

• Microsphere concentration

• Ligand concentration

• EDC concentration

• NHS concentration

• Activation pH

• Coupling-buffer composition

• Reaction temperature

• Incubation time

• Mixing efficiency

• Blocking conditions

Small-scale optimization is recommended before production scale-up.

Immunoassay Development Tips

For consistent results:

• Use purified antibodies or antigens.

• Avoid unnecessary carrier proteins.

• Resuspend particles before sampling.

• Maintain mixing during coupling.

• Optimize EDC/NHS concentration.

• Control activation pH.

• Optimize ligand loading.

• Block residual reactive sites.

• Evaluate nonspecific binding.

• Test coupled-particle stability.

CPS/DVB60UM vs. Smaller Carboxyl Microspheres

The preferred particle size depends on binding capacity, assay kinetics and handling requirements.

Crosslinked PS/DVB vs. Standard PS Microspheres

DVB-crosslinked polystyrene is widely used in larger PS microspheres when improved structural stability is required.

Covalent Coupling vs. Passive Adsorption

Quality Control for Bulk Production

For immunoassay reagent development, recommended evaluation items include:

• Particle-size consistency

• Suspension uniformity

• Solid-content consistency

• Surface carboxyl functionality

• Coupling efficiency

• Ligand loading

• Nonspecific binding

• Signal-to-background ratio

• Assay sensitivity

• Storage stability

• Batch-to-batch reproducibility

Why Choose SHBC CPS/DVB60UM?

• 60 μm carboxyl crosslinked PS/DVB microspheres

• High 10% solid-content suspension

• Reactive COOH surface

• Suitable for EDC/NHS coupling

• Designed for antibody and protein immobilization

• Suitable for immunoassay development

• Samples available for evaluation

• Stable batch manufacturing

• Bulk production capability

• Custom quantity and packaging support

Storage and Handling

Recommended practices include:

• Keep the container tightly sealed.

• Mix thoroughly before sampling.

• Resuspend settled particles before use.

• Avoid unnecessary freezing.

• Prevent microbial contamination.

• Use clean pipettes and containers.

• Avoid conditions causing irreversible aggregation.

• Validate storage conditions after biomolecule coupling.

Frequently Asked Questions

What is CPS/DVB60UM?

CPS/DVB60UM is a 60 μm carboxyl-functional crosslinked PS/DVB microsphere product supplied at 10% solids.

What does PS/DVB mean?

PS/DVB means polystyrene crosslinked with divinylbenzene.

Why use DVB crosslinking?

DVB creates a more rigid polymer network and helps maintain structural stability in larger polystyrene microspheres.

What is the function of the carboxyl surface?

Surface carboxyl groups provide reactive sites for covalent attachment of antibodies, proteins and other amine-containing biomolecules.

Can antibodies be coupled to CPS/DVB60UM?

Yes. Antibodies can be immobilized using optimized EDC or EDC/NHS coupling chemistry.

Can CPS/DVB60UM be used for immunoassays?

Yes. It can be evaluated for bead-based antibody, antigen and biomarker-detection applications.

What is the solid content?

CPS/DVB60UM is supplied at 10% solids.

Can amino-modified oligonucleotides be coupled?

Yes. Amino-modified DNA or RNA can be evaluated for carbodiimide-mediated coupling to the carboxyl surface.

Why choose 60 μm instead of smaller microspheres?

60 μm particles provide easier handling, washing and recovery. Smaller particles generally provide more surface area per unit mass.

Do 60 μm microspheres require mixing?

Yes. Larger particles settle faster, so regular mixing is recommended during coupling and assay reactions.

Is coupling optimization necessary?

Yes. EDC/NHS concentration, activation pH, ligand loading, reaction time and blocking conditions should be optimized for each project.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk supply for immunoassay developers, biotechnology companies and research organizations.

Request CPS/DVB60UM Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for CPS/DVB60UM samples, technical documentation and bulk quotations.

Please provide:

• Target antibody or protein

• Assay format

• Coupling chemistry

• Required microsphere quantity

• Target application

• Development stage

• Estimated annual demand

CPS/DVB60UM is supplied for research and process development. Final diagnostic applications should be validated by the customer.