Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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PM10UM-300A
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SHBC
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10µm
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5g 10g 50g 100g 1000g
10µm Porous Crosslinked PMMA Microspheres with 300Å Pores
SHBC PM10UM-300A is a porous crosslinked polymethyl methacrylate microsphere with a nominal particle size of 10µm and a pore size of 300Å.
The controlled porous structure, rigid polymer matrix and moderately polar methacrylate surface make it suitable for chromatography packing, biomolecule separation, adsorbent development and custom stationary-phase research.
Product Overview
PM10UM-300A is manufactured from a crosslinked PMMA-based polymer matrix.
The 10µm particle size provides a practical balance between separation efficiency, column pressure and preparative loading. The 300Å pores provide internal surface access for peptides, oligonucleotides, proteins and other biomolecules.
The product can be supplied as bulk porous microsphere media for analytical research, preparative purification and chromatography-material development.
Key Features and Benefits
Nominal particle size of 10µm
Controlled pore size of 300Å
Porous crosslinked PMMA matrix
Uniform spherical morphology
Accessible internal pore structure
Moderately polar methacrylate surface
Alternative selectivity to PS/DVB media
Suitable for chromatography column packing
Suitable for biomolecule purification
Compatible with further surface modification
Laboratory and bulk quantities available
Custom particle and pore sizes supported
Product Specifications
Parameter | Specification |
|---|---|
Product Name | Porous Crosslinked PMMA Microspheres |
Catalog Number | PM10UM-300A |
Brand | SHBC |
Particle Size | 10µm |
Pore Size | 300Å |
Equivalent Pore Diameter | 30nm |
Matrix | Crosslinked PMMA |
Particle Structure | Porous spherical microspheres |
Standard Surface | Methacrylate polymer surface |
Product Form | Bulk chromatography packing media |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Intended Use | Research and industrial separation |
Particle-size distribution, pore volume, specific surface area, crosslinking degree and supply form should be confirmed in the SHBC technical data sheet.
Benefits of 10µm Particles and 300Å Pores
Balanced Column Performance
The 10µm particle size provides useful separation efficiency while generally producing less column resistance than smaller analytical particles.
Wide-Pore Accessibility
The 300Å pore structure allows relatively large biomolecules to access the internal particle surface.
Increased Interaction Area
The porous structure provides more accessible internal area than nonporous PMMA particles.
Suitable for Preparative Purification
The combination of 10µm particles and wide pores is suitable for preparative column packing and process-development research.
Stable Crosslinked Structure
Crosslinking helps the polymer particles maintain their spherical shape and porous structure during packing, washing and chromatographic operation.
Recommended Applications
Peptide Purification
Suitable for analytical and preparative separation of synthetic peptides, biological peptides and peptide-related impurities.
Oligonucleotide Separation
Can be evaluated for purification of DNA or RNA oligonucleotides under optimized chromatographic conditions.
Protein Purification
The 300Å pores provide internal access for proteins, protein fragments and selected larger biomolecules.
Natural Product Separation
Suitable for method development involving plant extracts, fermentation products and other complex natural mixtures.
Pharmaceutical Intermediate Purification
Can be used for separating active compounds, intermediates and process-related impurities.
Polymeric Reversed-Phase Chromatography
The methacrylate polymer matrix can provide different retention and selectivity from conventional silica or highly hydrophobic PS/DVB media.
Size Exclusion Media Development
The controlled porous structure can be evaluated as a base material for size exclusion chromatography after suitable surface treatment and method validation.
Solid-Phase Extraction
Can be developed as a porous polymer sorbent for sample cleanup, concentration and impurity removal.
Functional Chromatography Media
The PMMA matrix can be further modified to prepare ion exchange, affinity, hydrophobic interaction or mixed-mode separation media.
PMMA vs. PS/DVB Chromatography Media
Feature | Porous PMMA | Porous PS/DVB |
|---|---|---|
Polymer Surface | Moderately polar | Strongly hydrophobic |
Main Selectivity | Alternative polymer selectivity | Strong hydrophobic retention |
Biomolecule Interaction | Potentially milder retention | Generally stronger retention |
Surface Modification | Available | Available |
Typical Use | Biomolecules and polar compounds | Hydrophobic compounds and RPC |
Matrix Type | Methacrylate polymer | Styrene-divinylbenzene polymer |
PMMA media may be preferred when PS/DVB provides excessive hydrophobic retention or when alternative selectivity is required.
Actual chromatographic performance depends on the analyte, mobile phase, pore structure and surface chemistry.
Advantages of the PMMA Matrix
Alternative Chromatographic Selectivity
The ester-containing methacrylate structure provides different surface polarity from aromatic PS/DVB materials.
Reduced Excessive Hydrophobic Retention
The PMMA matrix can provide milder interaction for selected proteins, peptides and polar analytes.
Flexible Surface Modification
The polymer surface can be activated or coated with functional groups and application-specific ligands.
No Silanol Surface
PMMA-based particles do not contain the residual silanol groups found on conventional silica materials.
Suitable for Custom Resin Development
The porous matrix can serve as a base particle for developing specialized chromatography and adsorption media.
Chromatography Packing Applications
PM10UM-300A can be evaluated for packing:
Analytical chromatography columns
Semi-preparative columns
Preparative purification columns
Peptide purification columns
Oligonucleotide purification columns
Protein separation columns
Solid-phase extraction cartridges
Custom research columns
Functionalized chromatography resins
This product is supplied as bulk microsphere media unless a prepacked column is specifically ordered.
General Column Packing Procedure
Select a compatible slurry liquid.
Fully wet and disperse the PMMA microspheres.
Prepare a uniform slurry without visible aggregates.
Remove trapped air when necessary.
Transfer the slurry into the column or packing reservoir.
Pack using controlled flow or pressure.
Avoid exceeding the validated pressure limit.
Flush the packed bed with the working mobile phase.
Equilibrate until pressure and baseline are stable.
Test column efficiency with a suitable standard.
Packing pressure, slurry concentration and solvent should be optimized for the selected column hardware.
Method Development Considerations
Chromatographic performance may be influenced by:
Analyte molecular size
Analyte polarity
Mobile-phase composition
Organic solvent concentration
Buffer pH
Ionic strength
Column temperature
Sample loading
Flow rate
Surface functionalization
Small-scale screening is recommended before preparative or industrial scale-up.
Surface Functionalization Options
SHBC can provide customized porous PMMA microspheres with different surface properties.
Available options may include:
Carboxyl groups
Amino groups
Hydroxyl groups
Epoxy groups
Quaternary ammonium groups
Sulfonic acid groups
Hydrophobic coatings
Hydrophilic coatings
Affinity ligands
Mixed-mode functional groups
Custom molecules
Functionalized products can be developed for ion exchange, affinity purification, hydrophobic interaction, enzyme immobilization and specialized adsorption.
Why Choose SHBC
Controlled Particle Manufacturing
SHBC applies controlled polymerization, pore formation and particle-classification processes.
Defined Particle and Pore Size
PM10UM-300A combines a 10µm particle size with a controlled 300Å porous structure.
Flexible Product Customization
Particle size, pore size, crosslinking degree, surface chemistry and packaging can be customized.
Reliable Batch Consistency
Controlled production and quality inspection support consistent product performance between batches.
Scale-Up Manufacturing
SHBC supports laboratory evaluation, pilot-scale purification and bulk production.
Technical Support
Technical assistance is available for media selection, slurry preparation, column packing and surface modification.
Custom and Bulk Supply
Shanghai SanYu Biotechnology Co., Ltd. supplies porous PMMA microspheres to pharmaceutical companies, biotechnology manufacturers, chromatography developers and research institutions.
Available customization includes:
Custom particle size
Custom pore size
Custom pore volume
Custom specific surface area
Custom crosslinking degree
Surface functionalization
Custom supply form
Custom packaging
Pilot-scale production
Bulk manufacturing
OEM and ODM services
Batch quality documentation
Please provide the target analyte, separation mode, column dimensions and estimated order quantity when requesting a quotation.
Frequently Asked Questions
What is PM10UM-300A?
PM10UM-300A is a porous crosslinked PMMA microsphere with a nominal particle size of 10µm and a pore size of 300Å.
What does 300Å mean?
It represents a nominal pore diameter of approximately 30nm inside the microspheres.
What is the benefit of 300Å pores?
The wide pores provide internal access for peptides, oligonucleotides, proteins and other relatively large analytes.
What is the advantage of 10µm particles?
The 10µm size provides a balance between chromatographic efficiency, loading capacity and operating pressure.
Is PM10UM-300A suitable for protein purification?
Yes. It can be evaluated for protein and protein-fragment separation when the pore structure and surface chemistry match the target molecule.
Can it be used for reversed-phase chromatography?
Yes. Porous methacrylate polymer media can be used for polymeric reversed-phase chromatography under optimized conditions.
How is PMMA different from PS/DVB?
PMMA has a more polar methacrylate surface, while PS/DVB generally provides stronger hydrophobic retention.
Can it be used for ion exchange chromatography?
The standard material does not contain ion-exchange groups. Charged surface groups can be customized.
Is this a finished chromatography column?
No. PM10UM-300A is supplied as bulk packing media unless a prepacked column is specifically requested.
Can SHBC customize the pore size?
Yes. Particle size, pore size, crosslinking degree and surface chemistry can be customized.
Is bulk production available?
Yes. SHBC supports samples, pilot-scale quantities and bulk manufacturing.
Storage and Handling
Store PM10UM-300A according to the SHBC technical documentation.
Keep the product tightly sealed and protected from contamination. Avoid exposure to unvalidated solvents, temperatures, pressures and strong oxidizing agents.
Request a Sample or Quotation
Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized specifications or bulk pricing.
Product: 10µm Porous Crosslinked PMMA Microspheres
Catalog Number: PM10UM-300A
Brand: SHBC
Particle Size: 10µm
Pore Size: 300Å
Matrix: Crosslinked PMMA
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
For research and industrial chromatography use only.