15um Carboxyl Green-Fluo Microspheres CFG15UM-10 1%

15um Carboxyl Green-Fluo Microspheres CFG15UM-10

SHBC CFG15UM-10 are 15um green fluorescent microspheres with reactive carboxyl groups on the particle surface.

With excitation at 470/488nm and emission at 525nm, these microspheres provide strong particle-level fluorescence for flow cytometry, fluorescence microscopy, particle tracking, cell interaction studies and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Bright green emission at 525nm

• Compatible with 470nm and 488nm excitation

• Reactive carboxyl surface

• Strong fluorescence per particle

• Easy microscopic observation

• Clear particle discrimination

• Large surface area for biomolecule loading

• Suitable for research and batch production

Why Choose 15um Fluorescent Microspheres?

The large 15um particle size provides strong fluorescence and easy particle identification.

Key benefits include:

• Easy particle counting

• Clear flow cytometry gating

• Strong single-particle fluorescence

• Convenient microscopic tracking

• Large coupling surface

• Good recovery by centrifugation

• Easy separation from smaller particles or cells

Carboxyl Surface for Biomolecule Coupling

Surface carboxyl groups can be activated for covalent conjugation with primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amine-containing molecules

EDC/NHS chemistry can be used to form stable amide bonds between the microspheres and target biomolecules.

Main Applications

Flow Cytometry

The 15um particle size and bright green fluorescence support clear particle identification in compatible flow cytometry systems.

Typical uses include:

• Fluorescent particle counting

• Particle population analysis

• Binding assay development

• Fluorescence channel evaluation

• Flow cytometry method development

Fluorescence Microscopy

CFG15UM-10 can be easily visualized using suitable fluorescence microscopes.

Applications include:

• Particle localization

• Fluorescence imaging

• Particle movement analysis

• Surface binding studies

• Material distribution research

Cell–Particle Interaction Studies

The microspheres can be evaluated for research involving interactions between cells and large fluorescent particles.

Potential applications include:

• Cell surface binding

• Immune cell interaction

• Particle adhesion studies

• Selected phagocytosis models

• Cell–material interface research

Particle size compatibility should be confirmed for each cell type and experimental model.

Particle Tracking and Flow Visualization

Bright fluorescence and easy optical recognition make CFG15UM-10 suitable for:

• Fluid-flow visualization

• Filtration studies

• Microfluidic tracking

• Particle transport research

• Material distribution analysis

• Porous-media research

Particle-Based Binding Assays

Antibodies, antigens or proteins can be immobilized on the carboxyl surface to prepare fluorescent capture particles.

Potential assay formats include:

• Fluorescent binding assays

• Antibody detection

• Antigen detection

• Agglutination research

• Ligand–receptor interaction studies

Tissue Perfusion and Distribution Research

Large fluorescent microspheres may be evaluated as non-radioactive tracers in tissue distribution and experimental perfusion studies.

Final suitability, particle concentration and experimental procedures must be validated by the researcher.

Biosensors and Microfluidics

The functional surface supports ligand immobilization for biosensors, biochips and microfluidic systems.

The strong green signal helps visualize particle capture, movement and distribution.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling and fluorescence performance.

Coupling pH, reagent concentration, reaction time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix gently before use.

• Protect from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the particles to dry.

• Avoid freezing and repeated freeze–thaw cycles.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Application Notes

For flow cytometry, optimize:

• Particle concentration

• Sample dispersion

• Flow rate

• Detector settings

• Fluorescence filters

• Gating strategy

For cell studies, validate particle size, particle-to-cell ratio, incubation time and washing conditions.

CFG15UM-10 is larger than particles normally used in lateral flow assays. It is not recommended as a standard LFIA reporter without complete membrane migration validation.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Particle size

• Fluorescence wavelength

• Fluorescence intensity

• Carboxyl density

• Alternative surface groups

• Solids content

• Suspension buffer

• Packaging volume

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, particle analysis and biological research.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk production

Frequently Asked Questions

What is CFG15UM-10?

CFG15UM-10 is a 15um carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.

Is CFG15UM-10 compatible with a 488nm laser?

Yes. It can be excited using a suitable 488nm laser and detected through a compatible green fluorescence channel.

Can the microspheres be coupled with antibodies?

Yes. Surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFG15UM-10 suitable for flow cytometry?

Yes. Its large particle size and green fluorescence support particle counting, binding analysis and flow cytometry method development.

Can it be used for fluorescence microscopy?

Yes. The 15um particle size provides easy visualization, localization and particle tracking.

Can it be used for cell uptake studies?

The particles may be evaluated for cell binding and selected phagocytosis models. Compatibility should be validated for the target cell type.

Is it suitable for lateral flow assays?

The 15um particle size is larger than standard LFIA reporter particles. Membrane migration and assay compatibility must be fully validated.

Can SHBC provide samples and bulk quantities?

Yes. SHBC supports research samples, pilot-scale evaluation and bulk manufacturing.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 15um Carboxyl Green-Fluo Microspheres
Catalog Number: CFG15UM-10
Particle Size: 15um
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.