15um Carboxyl Red-Fluo Microspheres CFR15UM-10

15um Carboxyl Red-Fluo Microspheres CFR15UM-10

SHBC CFR15UM-10 are 15 μm carboxyl-functionalized red fluorescent microspheres developed for flow cytometry, fluorescence imaging, particle-based assays and biomolecule immobilization.

The microspheres feature excitation at 535 nm, emission at 610 nm and 1% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size, size distribution, carboxyl content and fluorescence data are available in the Certificate of Analysis.

Red Fluorescence at 535/610nm

CFR15UM-10 produces red fluorescence with excitation at approximately 535 nm and emission centered at approximately 610 nm.

The optical profile is suitable for compatible flow cytometers, fluorescence microscopes, imaging systems and fluorescence readers.

Key Advantages

Uniform 15um Particle Size

Controlled particle sizing supports repeatable fluorescence detection, biomolecule coupling and particle analysis.

Strong Signal per Particle

The 15 μm particle body provides a strong fluorescent signal for clear detection and imaging.

Reactive Carboxyl Surface

Surface COOH groups support covalent immobilization of antibodies, antigens, proteins, peptides and other amino-containing molecules.

Large Coupling Surface

The large particle diameter provides substantial surface area per particle for biomolecule immobilization.

Easy Separation and Washing

Micrometer-sized particles can be conveniently recovered by centrifugation during coupling and washing procedures.

Custom and Bulk Supply

SHBC supports samples, customized specifications, pilot production and industrial batch supply.

Main Applications

Flow Cytometry

CFR15UM-10 can be evaluated for fluorescence channel testing, particle counting, gating development and bead-based assays.

Fluorescence Microscopy

The red fluorescent signal supports particle localization, distribution and imaging studies.

Particle-Based Immunoassays

Antibodies or antigens can be immobilized on the carboxyl surface for suspension immunoassays and agglutination research.

Biomolecule Immobilization

Suitable for covalent coupling of proteins, enzymes, peptides and other amino-containing molecules.

Cell–Particle Interaction Studies

Can be evaluated in particle adhesion, cellular contact and selected uptake studies.

Fluid and Particle Tracing

Suitable for selected fluid-flow, filtration, microchannel and particle-transport research.

Instrument Evaluation

Can be used in fluorescence detection, particle counting and imaging method development.

Biomolecule Coupling

Surface carboxyl groups can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.

A typical workflow includes:

1. Wash and redisperse the microspheres.

2. Activate the carboxyl groups with EDC and NHS.

3. Add the antibody or target protein.

4. Block remaining reactive sites.

5. Wash and resuspend the conjugated particles.

6. Evaluate coupling and fluorescence performance.

Coupling pH, reagent concentration, protein loading and reaction time should be optimized for each biomolecule.

Coupling Benefits

CFR15UM-10 provides:

• Reactive carboxyl groups

• Large coupling surface

• Stable covalent immobilization

• Easy centrifugation and washing

• Strong fluorescence detection

• Flexible ligand modification

• Compatibility with bead-based assays

• Custom conjugation support

Flow Cytometry Considerations

Recommended parameters to optimize include:

• Particle concentration

• Excitation source

• Emission filter

• Sample flow rate

• FSC and SSC settings

• Detector voltage

• Gating strategy

• Doublet discrimination

• Particle aggregation

Use an optical configuration compatible with 535 nm excitation and emission near 610 nm.

Because the particles are 15 μm, confirm that the instrument fluidics and flow cell can process the particle size.

Cell Study Considerations

For cell-related research, evaluate:

• Particle-to-cell ratio

• Cell type and size

• Incubation time

• Surface ligand density

• Culture conditions

• Washing efficiency

• Particle internalization

• Background fluorescence

The 15 μm particles may be too large for uptake by some cell types. Application suitability should be confirmed using the selected cell model.

Dispersion and Handling

Before use:

• Mix by gentle inversion or controlled vortexing

• Redisperse settled particles completely

• Avoid excessive foam

• Protect the suspension from strong light

• Prevent the microspheres from drying

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

• Use clean laboratory equipment

Controlled bath sonication may be used to break weak aggregates. Avoid aggressive probe sonication.

Centrifugation and Washing

The 15 μm particles can generally be recovered by low-speed centrifugation.

Optimize centrifugation according to:

• Suspension volume

• Buffer viscosity

• Particle concentration

• Tube type

• Required recovery rate

Avoid excessive centrifugation that may produce a difficult-to-redisperse pellet.

Application Limitations

CFR15UM-10 is generally unsuitable for conventional nitrocellulose membrane migration because of its large particle size.

For standard fluorescent lateral flow assays, submicron fluorescent microspheres are normally more suitable.

The product is better suited to suspension assays, flow cytometry, fluorescence imaging, particle tracing and biomolecule coupling.

Custom Carboxyl Fluorescent Microspheres

SHBC can customize fluorescent microspheres according to research and production requirements.

Customization options may include:

• Custom particle size

• Custom fluorescence wavelength

• Different fluorescence colors

• Carboxyl group density

• Solid content

• Dispersion buffer

• Surface modification

• Biomolecule conjugation

• Custom packaging

• OEM and ODM production

Please provide the target application, required wavelength, biomolecule and order quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Microscopy images

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFR15UM-10?

CFR15UM-10 is a 15 μm carboxyl-functionalized red fluorescent microsphere suspension with 1% solid content.

What are the excitation and emission wavelengths?

The excitation wavelength is 535 nm and the emission wavelength is 610 nm.

What is CFR15UM-10 mainly used for?

It is suitable for flow cytometry, fluorescence microscopy, particle-based immunoassays, biomolecule coupling and particle-tracing studies.

Can antibodies be coupled to CFR15UM-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling chemistry is recommended?

EDC/NHS chemistry is commonly used to activate surface carboxyl groups for protein immobilization.

Is CFR15UM-10 suitable for flow cytometry?

Yes, provided the instrument fluidics can process 15 μm particles. Optical filters, scatter settings and gating conditions should be optimized.

Can CFR15UM-10 be used for cell uptake studies?

It can be evaluated with suitable cell models. The particle size may be too large for uptake by some cell types.

Is CFR15UM-10 suitable for lateral flow assays?

The 15 μm particles are generally too large for conventional nitrocellulose membrane migration. Smaller fluorescent microspheres are recommended for standard LFIA formats.

How should CFR15UM-10 be dispersed?

Use gentle inversion, controlled vortexing or compatible bath sonication while avoiding excessive foam and particle damage.

Can the particles be separated by centrifugation?

Yes. The particles can generally be recovered by centrifugation. Speed and time should be optimized for the working volume and buffer.

Can CFR15UM-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer, surface ligand and packaging can be customized.

Are samples and bulk quantities available?

Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.

Request a Sample or Quotation

Contact SHBC for CFR15UM-10 samples, technical specifications, conjugation support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 15um Carboxyl Red-Fluo Microspheres
Catalog Number: CFR15UM-10
Particle Size: 15 μm
Solid Content: 1%
Excitation/Emission: 535/610 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.