18um Carboxyl Green-Fluo Microspheres CFG18UM-10 1%

18um Carboxyl Green-Fluo Microspheres CFG18UM-10

SHBC CFG18UM-10 are 18um green fluorescent microspheres with carboxyl-functionalized surfaces.

The microspheres provide green fluorescence with excitation at 470/488nm and emission at 525nm. They are supplied as a 1% solids suspension for fluorescence imaging, particle tracking, flow cytometry research and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Green fluorescence at 525nm

• Compatible with 470nm and 488nm excitation

• Reactive carboxyl surface

• Easy visualization under fluorescence microscopy

• Strong particle-level optical signal

• Large surface area for biomolecule loading

• Easy particle counting and tracking

• Suitable for custom and bulk production

Why Choose 18um Green Fluorescent Microspheres?

The large 18um particle size supports easy optical recognition and clear particle discrimination.

CFG18UM-10 is especially suitable for applications requiring:

• Visible individual particles

• Strong fluorescence per particle

• Large biomolecule coupling surfaces

• Easy microscopic localization

• Particle transport observation

• Separation from smaller particles

The particles may sediment faster than smaller microspheres. Gentle mixing before sampling is recommended.

Carboxyl Surface for Covalent Coupling

CFG18UM-10 contains carboxyl groups that can be activated for covalent conjugation with primary amine-containing biomolecules.

Suitable ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amine-containing molecules

EDC/NHS chemistry may be used to form stable amide bonds between the microsphere surface and the target ligand.

Main Applications

Fluorescence Microscopy

The 18um particle size provides easy visualization using compatible fluorescence microscopes.

Typical applications include:

• Particle localization

• Fluorescence imaging

• Surface binding studies

• Particle movement analysis

• Material distribution research

• Microsphere counting

Flow Cytometry Research

CFG18UM-10 can be evaluated for particle counting, fluorescence analysis and binding assay development.

Potential uses include:

• Fluorescent particle detection

• Particle population analysis

• Surface binding assays

• Fluorescence channel evaluation

• Method development

Instrument fluidics, nozzle size and particle-size compatibility should be confirmed before use.

Particle Tracking and Fluid Tracing

The green fluorescent signal supports visual monitoring of particle movement and distribution.

Applications include:

• Microfluidic flow visualization

• Filtration studies

• Fluid transport research

• Porous-media analysis

• Mixing and dispersion studies

• Environmental tracer research

Cell–Particle Interaction Studies

CFG18UM-10 can be evaluated for research involving the interaction of cells with large fluorescent particles.

Potential applications include:

• Cell surface binding

• Particle adhesion

• Immune cell interaction

• Cell–material interface research

• Selected phagocytosis models

Particle size and biological compatibility must be validated for each cell model.

Fluorescent Binding Assays

Antibodies, antigens or proteins can be immobilized on the carboxyl surface to prepare fluorescent capture particles.

Potential formats include:

• Antibody binding assays

• Antigen detection research

• Ligand–receptor studies

• Fluorescent agglutination

• Particle-based bioassays

Biosensors and Microfluidic Systems

The functional surface supports ligand immobilization for biosensors, biochips and microfluidic platforms.

The large particle size also supports easy observation of particle capture, movement and release.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling and fluorescence performance.

Reaction pH, reagent concentration, coupling time and blocking conditions should be optimized for each ligand.

Handling Recommendations

• Mix gently before sampling.

• Protect the suspension from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the particles to dry.

• Avoid freezing and repeated freeze–thaw cycles.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Application Notes

For flow cytometry, verify that the instrument can process 18um particles without fluidic blockage.

For microscopy and tracking studies, optimize particle concentration, exposure settings and suspension conditions.

CFG18UM-10 is larger than standard fluorescent particles used in lateral flow assays. It is not recommended as a conventional LFIA reporter without complete membrane migration validation.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from research evaluation to batch manufacturing.

Customization options include:

• Particle size

• Fluorescence wavelength

• Fluorescence intensity

• Surface carboxyl density

• Alternative functional groups

• Solids content

• Suspension buffer

• Packaging volume

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, particle analysis, immunoassays and biological research.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk manufacturing

Frequently Asked Questions

What is CFG18UM-10?

CFG18UM-10 is an 18um carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.

Is CFG18UM-10 compatible with a 488nm laser?

Yes. It can be excited with a suitable 488nm laser and detected through a compatible green fluorescence channel.

Can CFG18UM-10 be coupled with antibodies?

Yes. Its carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is it suitable for flow cytometry?

It can be evaluated using flow cytometers with fluidics and nozzle dimensions compatible with 18um particles.

Can it be used for fluorescence microscopy?

Yes. The large particle size supports easy visualization, counting, localization and tracking.

Can it be used for cell studies?

It can be evaluated for cell surface binding, particle adhesion and selected cell–particle interaction models. Suitability must be validated for the target cell type.

Is it suitable for lateral flow assays?

The 18um particle size is larger than conventional LFIA reporter particles. Membrane migration and assay compatibility would require complete validation.

Can SHBC provide samples and bulk quantities?

Yes. SHBC supports research samples, pilot-scale evaluation and bulk manufacturing.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 18um Carboxyl Green-Fluo Microspheres
Catalog Number: CFG18UM-10
Particle Size: 18um
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.