1um Carboxyl Green-Fluo Microspheres CFG1UM-10 1%

1um Carboxyl Green-Fluo Microspheres CFG1UM-10

SHBC CFG1UM-10 Carboxyl Green-Fluo Microspheres are 1um fluorescent polymer particles with carboxyl-functionalized surfaces.

The microspheres provide bright green fluorescence with excitation at 470/488nm and emission at 525nm. They are supplied as a 1% solids suspension for immunoassays, flow cytometry, fluorescence imaging, biosensing and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Bright green fluorescence at 525nm

• Compatible with 470nm and 488nm excitation

• Carboxyl surface for covalent conjugation

• Uniform 1um particle size

• Large surface area per particle

• Good optical visibility

• Stable suspension and fluorescence

• Suitable for research and batch production

Carboxyl Surface for Biomolecule Coupling

CFG1UM-10 contains carboxyl groups for the immobilization of amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amino-containing molecules

The surface carboxyl groups can be activated using EDC/NHS chemistry to form stable amide bonds with target biomolecules.

Main Applications

Fluorescence Immunoassays

CFG1UM-10 can be used as a fluorescent signal carrier in sandwich immunoassays, competitive assays and particle-based detection systems.

Flow Cytometry

The 1um particle size and green fluorescent signal are suitable for flow cytometry research, instrument evaluation and fluorescence-based particle analysis.

Fluorescence Microscopy

The microspheres offer good optical visibility for microscopy, imaging, localization and particle-tracking studies.

Antibody and Protein Conjugation

Surface carboxyl groups support covalent antibody, protein and peptide immobilization for fluorescent probe preparation.

Cell Labeling and Tracking

CFG1UM-10 can be evaluated for cell uptake, phagocytosis, cell labeling and biological transport studies.

Biosensors and Biochips

The microspheres can serve as fluorescent carriers in biosensors, microarrays, biochips and microfluidic detection platforms.

Multiplex Assay Development

Different particle sizes or fluorescence signals can be combined to develop distinguishable particle populations for multiplex detection research.

Diagnostic Reagent Development

CFG1UM-10 is suitable for fluorescence-based diagnostic reagent research, assay optimization and pilot-scale production.

Why Choose 1um Fluorescent Microspheres?

The 1um particle size offers strong fluorescence and easy optical detection.

Compared with smaller particles, 1um microspheres provide:

• Higher fluorescence per particle

• More surface area for biomolecule loading

• Easier visualization under microscopy

• Clear particle identification in flow-based systems

• Good suitability for cell and particle-tracking studies

For membrane-based assays, migration performance should be evaluated with the selected membrane pore size, buffer and surfactant system.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling efficiency and fluorescence performance.

Reaction pH, reagent concentration, coupling time and blocking conditions should be optimized for each application.

Handling Recommendations

• Mix thoroughly before use.

• Protect from strong light.

• Avoid prolonged probe sonication.

• Prevent the microspheres from drying.

• Avoid repeated freezing and thawing.

• Use low-binding laboratory consumables.

• Verify buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Particle size

• Fluorescence wavelength

• Surface carboxyl density

• Alternative functional groups

• Solids content

• Suspension buffer

• Packaging volume

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for immunoassays, fluorescence detection, bioseparation and diagnostic reagent development.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch consistency

• Technical coupling support

• Research samples

• Flexible packaging

• Bulk manufacturing

Frequently Asked Questions

What is CFG1UM-10?

CFG1UM-10 is a 1um carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The recommended excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.

Can CFG1UM-10 be coupled with antibodies?

Yes. The surface carboxyl groups can be activated for covalent coupling with antibody amino groups.

Is it suitable for flow cytometry?

Yes. Its 1um particle size and green fluorescence make it suitable for flow cytometry research and particle-based fluorescence analysis.

Can it be used for fluorescence microscopy?

Yes. The particles provide strong optical visibility for fluorescence imaging, localization and tracking studies.

Is it suitable for lateral flow assays?

The product may be evaluated for membrane-based assays. Migration performance should be optimized according to membrane pore size, buffer composition and assay format.

Can SHBC provide samples?

Yes. Samples are available for coupling tests, formulation development and application evaluation.

Is bulk production available?

Yes. SHBC supports pilot-scale orders and bulk production for research institutions and diagnostic reagent manufacturers.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, functional group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 1um Carboxyl Green-Fluo Microspheres
Catalog Number: CFG1UM-10
Particle Size: 1um
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customization and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.