1um Streptavidin Magnetic Beads MSA1UM-10 1%

1µm Streptavidin Magnetic Beads MSA1UM-10

MSA1UM-10 is a 1µm streptavidin-functional magnetic bead suspension supplied at 1% solids.

When the solids content is expressed as weight per volume, 1% corresponds to approximately 10mg of magnetic beads per milliliter of suspension.

The product is developed for the capture and immobilization of biotinylated antibodies, antigens, proteins, peptides, aptamers, oligonucleotides and other biotin-labelled molecules.

MSA1UM-10 can be evaluated as a magnetic solid phase in chemiluminescence immunoassay research, magnetic immunoassay development, biomolecule enrichment, process scale-up and batch reagent manufacturing.

Streptavidin immobilized on the bead surface binds biotinylated ligands without requiring the customer to activate the magnetic beads using EDC, NHS or glutaraldehyde. After loading the selected biotinylated capture molecule, the functionalized beads can be used for target capture, magnetic separation, washing and signal detection.

MSA1UM-10 is intended for biotechnology companies, immunoassay reagent developers, research institutions and manufacturing enterprises requiring a 1µm streptavidin magnetic bead raw material for laboratory evaluation, pilot production and bulk supply.

The finished reagent should be validated using the intended biotinylated ligand, sample matrix, magnetic separator, chemiluminescent detection chemistry and analyzer.

Product Overview

The streptavidin–biotin system provides a flexible method for immobilizing different biotinylated molecules on a common magnetic bead surface.

Commercial 1µm streptavidin magnetic beads are widely used to capture biotinylated antibodies, antigens, peptides, proteins and nucleic acids. This particle size is also commonly offered for automated and high-throughput magnetic workflows.

MSA1UM-10 is supplied at 1% solids to support:

• Biotinylated antibody immobilization;

• Biotinylated antigen immobilization;

• Magnetic sandwich immunoassays;

• Antibody-detection assays;

• Competitive immunoassays;

• Chemiluminescence immunoassay development;

• Biomolecule enrichment;

• Nucleic acid and aptamer capture;

• Magnetic separation research;

• Automated reagent development;

• Pilot-scale and batch manufacturing.

The product is a research and reagent-development raw material. It is not supplied as a finished diagnostic reagent.

MSA1UM-10 Product Specifications

The following product-specific parameters should be confirmed through the applicable technical data sheet and lot-specific certificate of analysis:

• Particle-size distribution;

• Streptavidin activity;

• Biotin-binding capacity;

• Magnetic collection performance;

• Suspension buffer;

• Blocking system;

• Preservative;

• Storage temperature;

• Shelf life;

• Lot-release specifications.

How Do Streptavidin Magnetic Beads Work?

Streptavidin is a biotin-binding protein that can be immobilized on a solid surface.

When a biotinylated antibody, antigen, protein or oligonucleotide is incubated with streptavidin magnetic beads, the biotin group binds to the streptavidin on the particle surface.

The result is a magnetic bead–streptavidin–biotin–ligand complex.

This structure combines two functions:

1. The biotinylated ligand provides biological recognition of the target.

2. The magnetic bead provides a solid phase for separation, washing and reagent handling.

The streptavidin–biotin interaction is widely used for capturing biotin-labelled antibodies, antigens and nucleic acids.

Example Using a Biotinylated Capture Antibody

A typical sandwich immunoassay workflow may include:

1. Biotinylate the capture antibody.

2. Incubate the biotinylated antibody with MSA1UM-10.

3. Remove unbound antibody.

4. Resuspend the antibody-loaded beads.

5. Incubate the beads with the sample.

6. Capture the target antigen.

7. Add a labelled detection antibody.

8. Magnetically collect and wash the immune complex.

9. Add the chemiluminescent detection reagent.

10. Measure the analytical signal.

Example Using a Biotinylated Antigen

For an antibody-detection assay:

1. Biotinylate the target antigen.

2. Immobilize the antigen on MSA1UM-10.

3. Incubate the antigen-loaded beads with the sample.

4. Capture target-specific antibodies.

5. Add a labelled secondary antibody.

6. Perform magnetic washing.

7. Measure the chemiluminescent signal.

Example Using a Biotinylated Oligonucleotide

Biotinylated oligonucleotides may be loaded onto MSA1UM-10 for:

• Nucleic acid capture;

• Hybridization assays;

• Aptamer-based detection;

• Target enrichment;

• Probe immobilization;

• Molecular diagnostic research.

Key Features of MSA1UM-10

Defined 1µm Particle Size

The 1µm particle format provides a small micrometer-scale magnetic carrier for biomolecule immobilization and magnetic separation.

Compared with larger particles of similar composition, a smaller particle diameter generally provides more geometric surface area per unit bead mass. The actual binding capacity, however, also depends on streptavidin density, surface accessibility, ligand size, degree of biotinylation and steric effects.

Thermo Fisher specifically notes that the practical binding capacity of 1µm streptavidin beads varies with molecular size, biotinylation method, charge and accessibility of the biotin group.

Streptavidin-Functional Surface

MSA1UM-10 provides a streptavidin affinity surface for capturing biotinylated ligands.

Potential ligands include:

• Biotinylated monoclonal antibodies;

• Biotinylated polyclonal antibodies;

• Biotinylated recombinant antigens;

• Biotinylated natural proteins;

• Biotinylated peptides;

• Biotinylated enzymes;

• Biotinylated aptamers;

• Biotinylated DNA probes;

• Biotinylated RNA probes;

• Other biotin-labelled recognition molecules.

1% Solids Formulation

The 1% suspension corresponds to approximately10mg/mL when expressed as w/v.

This concentration is comparable with several widely used commercial1µm streptavidin magnetic bead products supplied at approximately10mg/mL.

Approximate bead-mass calculations are:

• 10µL contains approximately0.1mg of beads;

• 50µL contains approximately0.5mg of beads;

• 100µL contains approximately1mg of beads;

• 1mL contains approximately10mg of beads.

These calculations assume that the stated1% concentration is w/v.

No Additional Bead Activation Required

For loading a properly biotinylated ligand, the end user does not normally need to activate the bead surface using EDC/NHS or another covalent coupling reagent.

This simplifies early-stage screening because different biotinylated capture molecules can be compared using the same magnetic bead platform.

Flexible Ligand Screening

MSA1UM-10 can be evaluated with different biotinylated antibodies, antigens or probes.

This supports:

• Capture-antibody screening;

• Antibody-pair selection;

• Antigen-construct comparison;

• Assay-target expansion;

• Multiproduct reagent development;

• Early feasibility studies.

Magnetic Solid-Phase Handling

After ligand loading and target capture, the beads can be collected using a compatible magnetic separator.

A typical process consists of:

• Bead dispensing;

• Sample incubation;

• Magnetic collection;

• Supernatant removal;

• Washing;

• Detection-reagent incubation;

• Additional magnetic washing;

• Signal generation.

Research-to-Production Supply

MSA1UM-10 can support:

• Initial feasibility testing;

• Biotinylated ligand screening;

• Immunoassay optimization;

• Automated analyzer evaluation;

• Stability studies;

• Pilot manufacturing;

• Lot-consistency assessment;

• Bulk raw-material procurement.

Why Choose 1% Streptavidin Magnetic Beads?

Industry-Comparable Starting Concentration

Several commercial 1µm streptavidin magnetic beads are supplied at approximately1% solids or10mg/mL. This gives assay developers a familiar starting concentration for method transfer and competitive comparison.

Convenient Bead-Mass Calculations

At approximately10mg/mL, calculating the required bead mass is straightforward.

For example:

• 100µL provides approximately1mg of beads;

• 500µL provides approximately5mg of beads;

• 1mL provides approximately10mg of beads.

Easier Direct Dispensing Than Higher-Solids Concentrates

Compared with a more concentrated5% suspension, a1% formulation may require less dilution before certain coupling or assay-development experiments.

The correct working concentration must still be optimized for the intended assay.

Suitable for Ligand-Loading Studies

The1% concentration can be used to screen different ratios of:

• Biotinylated ligand per milligram of beads;

• Bead mass per reaction;

• Bead concentration per test;

• Bead dosage per automated reaction cup.

Potential Compatibility With Automated CLIA Systems

Commercial1µm streptavidin beads are commonly positioned for automated and high-throughput workflows. MSA1UM-10 may therefore be evaluated for automated CLIA development, but dispensing, magnetic collection, aspiration, washing and resuspension must be validated on the intended analyzer.

Applications in Chemiluminescence Immunoassays

Magnetic Sandwich Immunoassays

A biotinylated capture antibody can be loaded onto MSA1UM-10.

The resulting antibody-functionalized beads capture the target antigen from the sample. A labelled detection antibody then forms a sandwich immune complex.

After magnetic separation and washing, the detection label generates a chemiluminescent signal.

Important development parameters include:

• Bead dosage;

• Biotinylated antibody loading;

• Degree of biotinylation;

• Capture-antibody activity;

• Sample volume;

• Incubation time;

• Detection-antibody concentration;

• Wash efficiency;

• Signal-to-background ratio.

Antibody-Detection Assays

A biotinylated antigen can be immobilized on MSA1UM-10 to capture target antibodies from a sample.

Potential research applications include:

• Infectious-disease antibody assays;

• Autoantibody assays;

• Vaccine-response research;

• Antibody-response monitoring;

• Recombinant antigen evaluation.

Competitive Immunoassays

Biotinylated antigens, haptens or small-molecule conjugates may be immobilized for competitive assay development.

This format may be appropriate when the target analyte cannot support a conventional two-antibody sandwich structure.

Indirect Immunoassay Formats

MSA1UM-10 may also be evaluated with:

• Biotinylated secondary antibodies;

• Biotinylated anti-species antibodies;

• Biotinylated affinity proteins;

• Biotinylated peptides;

• Biotinylated aptamers.

Immunomagnetic Enrichment

Ligand-loaded beads can be evaluated for enriching:

• Proteins;

• Antigens;

• Cells;

• Microorganisms;

• Extracellular particles;

• Other affinity-recognized targets.

Nucleic Acid and Aptamer Capture

Biotinylated DNA, RNA or aptamer probes may be immobilized for:

• Hybridization assays;

• Nucleic acid enrichment;

• Target capture before amplification;

• Aptamer-based detection;

• Biosensor development;

• Molecular diagnostic research.

Commercial1µm streptavidin beads are used to capture biotin-labelled antibodies, antigens and nucleic acids, confirming the flexibility of this surface chemistry across immunoassay and molecular biology workflows.

Compatible Biotinylated Antibodies and Biomolecules

Biotinylated Antibodies

Biotinylated antibodies are common capture ligands for streptavidin magnetic beads.

Important variables include:

• Antibody purity;

• Antibody concentration;

• Biotinylation chemistry;

• Degree of biotinylation;

• Position of biotin modification;

• Antibody aggregation;

• Retained antigen-binding activity;

• Original antibody buffer.

Excessive biotinylation may reduce antibody activity or create unfavourable surface orientation.

Insufficient biotinylation may reduce bead loading.

Biotinylated Antigens

Biotinylated antigens may be used for antibody-detection assays.

The developer should confirm that biotinylation does not modify or block an important epitope.

Biotinylated Peptides

Potential uses include:

• Antibody screening;

• Epitope analysis;

• Competitive immunoassays;

• Protein-interaction research;

• Affinity capture.

Biotinylated Oligonucleotides

Biotin may be introduced at the5′ end,3′ end or another validated position.

The selected modification position should preserve hybridization, target recognition or aptamer activity.

Biotinylated Proteins and Enzymes

Biotinylated recombinant proteins or enzymes may be used for:

• Protein-interaction studies;

• Enzyme assays;

• Affinity enrichment;

• Biosensor development;

• Calibration-material preparation.

Recommended Biotinylated Ligand Loading Workflow

The following workflow is a general development framework. It is not a validated product-specific protocol.

Step 1: Characterize the Ligand

Record:

• Ligand identity;

• Molecular weight;

• Concentration;

• Purity;

• Original formulation;

• Biotinylation method;

• Estimated degree of biotinylation;

• Biological activity;

• Aggregation status;

• Stabilizing additives.

Step 2: Remove Excess Free Biotin

Free biotin can occupy streptavidin-binding sites and reduce loading of the intended biotinylated antibody, protein or oligonucleotide.

Thermo Fisher advises removing excess free biotin because free biotin can bind streptavidin magnetic beads faster than larger biotinylated molecules.

Potential purification approaches include:

• Desalting;

• Dialysis;

• Size-exclusion chromatography;

• Ultrafiltration;

• HPLC or FPLC for suitable oligonucleotides;

• Another validated purification method.

Step 3: Fully Resuspend MSA1UM-10

Mix the bead suspension until homogeneous before sampling.

Possible methods include:

• Gentle inversion;

• Roller mixing;

• End-over-end rotation;

• Controlled vortexing;

• Another validated mixing method.

Avoid excessive foaming.

Step 4: Calculate the Required Bead Mass

Assuming a concentration of10mg/mL:

Bead mass = suspension volume × 10mg/mL

Prepare several bead quantities instead of evaluating only one condition.

Step 5: Wash or Equilibrate the Beads

Collect the beads using a compatible magnetic separator.

Remove the original suspension medium when required and equilibrate the beads in a validated loading buffer.

Step 6: Add the Biotinylated Ligand

Evaluate several ligand-to-bead ratios.

Suggested experimental groups may include:

• Low ligand loading;

• Medium ligand loading;

• High ligand loading;

• Different incubation times;

• Different temperatures;

• Different buffer compositions.

Step 7: Maintain Gentle Mixing

Use sufficient mixing to keep the beads distributed without damaging the biological ligand.

Step 8: Magnetically Collect the Loaded Beads

Standardize:

• Magnet type;

• Vessel;

• Reaction volume;

• Collection time;

• Collection position;

• Residual liquid volume.

Step 9: Retain the Supernatant

Retain the post-loading supernatant when ligand-loading efficiency needs to be measured.

Step 10: Wash the Loaded Beads

Remove:

• Unbound biotinylated ligand;

• Residual free biotin;

• Unwanted formulation components;

• Other unbound materials.

Step 11: Resuspend in the Final Buffer

Potential formulation components include:

• Buffer;

• Salt;

• Protein stabilizer;

• Synthetic polymer;

• Surfactant;

• Preservative;

• Sugar or polyol;

• Blocking component.

Step 12: Evaluate Functional Performance

Recommended tests include:

• Ligand-loading efficiency;

• Target-capture capacity;

• Blank signal;

• Positive signal;

• Signal-to-background ratio;

• Magnetic recovery;

• Redispersion;

• Precision;

• Accelerated stability;

• Real-time stability.

How to Optimize MSA1UM-10 in an Immunoassay

Optimize Bead Dosage

Compare several bead amounts in the complete assay.

Too few beads may limit target capture.

Too many beads may increase:

• Reagent cost;

• Nonspecific adsorption;

• Blank signal;

• Washing requirements;

• Residual bead interference;

• Instrument carryover.

Optimize Biotinylated Ligand Loading

Evaluate several amounts of biotinylated antibody or antigen per milligram of beads.

The condition with the highest chemical loading may not provide the best analytical performance.

Excessive loading can cause:

• Steric crowding;

• Reduced target accessibility;

• Higher nonspecific binding;

• Lower functional activity;

• Poorer storage stability.

Optimize Degree of Biotinylation

Compare ligands with different degrees of biotinylation.

Evaluate:

• Bead-loading efficiency;

• Retained biological activity;

• Target-capture performance;

• Blank signal;

• Positive signal;

• Precision;

• Stability.

Optimize Loading Time

The required loading time depends on:

• Ligand size;

• Biotin accessibility;

• Bead concentration;

• Mixing;

• Temperature;

• Buffer composition.

Optimize Blocking and Storage Buffer

Potential formulation variables include:

• Protein blockers;

• Synthetic blocking polymers;

• Nonionic surfactants;

• Ionic strength;

• Sugars;

• Polyols;

• Preservatives.

The final formulation should support bead dispersion, low background, streptavidin activity and ligand stability.

Optimize Magnetic Separation

Evaluate:

• Magnet strength;

• Magnet position;

• Collection time;

• Reaction-vessel geometry;

• Bead concentration;

• Buffer viscosity;

• Residual liquid;

• Bead loss during aspiration.

Optimize Washing

Compare:

• Wash-buffer composition;

• Surfactant concentration;

• Wash volume;

• Number of wash cycles;

• Mixing during washing;

• Magnetic collection time;

• Residual wash volume.

Optimize Automated Analyzer Parameters

For automated CLIA development, evaluate:

• Reagent-reservoir mixing;

• Bead-dispensing accuracy;

• Incubation mixing;

• Magnetic collection time;

• Aspiration height;

• Wash cycles;

• Residual volume;

• Bead redispersion;

• Carryover.

How to Evaluate Biotin-Binding Performance

Total Biotin-Binding Capacity

A labelled biotin reagent can be used to estimate available streptavidin-binding sites.

Commercial suppliers often report capacity as picomoles of biotin per milligram of magnetic particles, but the actual acceptance range for MSA1UM-10 must be established using its own validated method and production data.

Biotinylated Antibody Loading

Measure the antibody concentration before and after loading.

An approximate calculation is:

Loaded antibody = Initial antibody − Unbound antibody

Possible analytical methods include:

• UV absorbance;

• BCA assay;

• Bradford assay;

• Fluorescence measurement;

• ELISA;

• Another validated protein assay.

Functional Target-Capture Capacity

Chemical loading does not prove that the antibody or antigen remains biologically active.

Functional testing should measure actual target capture under representative assay conditions.

Non-Specific Binding

Compare:

• Unloaded streptavidin beads;

• Ligand-loaded beads;

• Blocked beads;

• Negative samples;

• Positive samples;

• Representative interfering substances.

Magnetic Recovery

Measure bead recovery after repeated magnetic separation and washing.

Immunoassay Performance

Evaluate:

• Blank signal;

• Negative-sample signal;

• Low-positive signal;

• High-positive signal;

• Signal-to-background ratio;

• Precision;

• Recovery;

• Linearity;

• Hook effect;

• Stability.

Quality Control for Research and Batch Production

Appearance and Dispersion

Inspect:

• Suspension appearance;

• Visible aggregation;

• Sedimentation behaviour;

• Homogeneity after mixing;

• Ease of redispersion;

• Stability during processing holds.

Particle Size and Distribution

Confirm the nominal1µm particle size using an appropriate validated method.

For batch production, monitor both average particle size and distribution.

Solids Content

Verify solids concentration because it affects:

• Bead-mass calculations;

• Ligand-loading calculations;

• Assay formulation;

• Filling accuracy;

• Production yield.

Streptavidin Activity

Use a standardized biotin-binding test to evaluate active streptavidin on the bead surface.

Biotin-Binding Capacity

Establish a validated assay and internal acceptance range.

Competitor capacity values should be used only as benchmarking information, not as the specification for MSA1UM-10.

Biotinylated Antibody Loading

Use a representative biotinylated antibody to evaluate practical loading performance.

Magnetic Collection

Standardize:

• Magnet;

• Vessel;

• Sample volume;

• Buffer;

• Bead concentration;

• Temperature;

• Collection time.

Non-Specific Binding

Evaluate background using representative proteins, antibodies and sample matrices.

Functional CLIA Performance

Recommended tests include:

• Blank signal;

• Negative-sample signal;

• Positive-sample signal;

• Signal-to-background ratio;

• Analytical sensitivity;

• Precision;

• Recovery;

• Linearity;

• Specificity;

• Stability.

Lot-to-Lot Consistency

For routine manufacturing, establish acceptance ranges for:

• Particle size;

• Solids content;

• Streptavidin activity;

• Biotin-binding capacity;

• Magnetic collection;

• Dispersion;

• Functional immunoassay performance.

Final material approval should be based on performance in the complete assay system.

Scale-Up and Bulk Supply for Immunoassay Manufacturers

Evaluation Stage

Evaluation samples can support screening of:

• Biotinylated capture antibodies;

• Biotinylated antigens;

• Bead dosage;

• Ligand-loading level;

• Blocking formulation;

• Magnetic separation;

• Assay sensitivity;

• Background signal.

Pilot-Scale Stage

Pilot quantities can support:

• Process confirmation;

• Reagent formulation;

• Analyzer compatibility testing;

• Stability studies;

• Filling-process evaluation;

• Initial lot-consistency testing.

Batch-Production Stage

Bulk-supply planning may include:

• Annual demand;

• Purchasing forecast;

• Packaging volume;

• Quality specifications;

• Technical documents;

• Quality documents;

• Reserved-lot requirements;

• Change-control expectations;

• Safety-stock planning.

Scale-Up Considerations

A laboratory loading procedure should not be scaled only by multiplying reagent volumes.

Revalidate:

• Vessel geometry;

• Mixing efficiency;

• Bead uniformity;

• Ligand-addition sequence;

• Incubation time;

• Temperature uniformity;

• Magnetic collection;

• Washing efficiency;

• Process hold time;

• Final filling uniformity.

Custom Streptavidin Magnetic Bead Services

SHBC can evaluate customized streptavidin magnetic bead requirements for biotechnology companies, research institutions and immunoassay manufacturers.

Potential customization options include:

• Alternative particle sizes;

• Customized solids content;

• Adjusted streptavidin loading;

• Customer-specific biotin-binding capacity targets;

• Alternative blocking systems;

• Customized suspension buffer;

• Alternative preservatives;

• Customer-specified pH;

• Special packaging volumes;

• Pilot-scale manufacturing;

• Bulk manufacturing;

• OEM packaging;

• Private-label services;

• Customer-specific quality testing.

Customization feasibility depends on the requested specification, intended application, validation requirements and order quantity.

Storage and Handling Recommendations

Store MSA1UM-10 according to the product label, technical data sheet and lot-specific certificate of analysis.

General recommendations include:

• Mix thoroughly before sampling;

• Maintain suspension uniformity during dispensing;

• Use clean and calibrated equipment;

• Avoid allowing the beads to dry;

• Prevent microbial and chemical contamination;

• Avoid freezing unless specifically validated;

• Avoid unnecessary exposure to extreme temperatures;

• Confirm buffer compatibility before buffer exchange;

• Prevent contamination with free biotin;

• Record the product lot number;

• Evaluate ligand-loaded bead stability separately.

Commercial1µm streptavidin magnetic bead suppliers commonly recommend refrigerated storage, mixing before use and avoidance of freezing. Product-specific storage conditions for MSA1UM-10 should be confirmed in its own documentation.

Frequently Asked Questions

What is MSA1UM-10?

MSA1UM-10 is a1µm streptavidin-functional magnetic bead suspension supplied at1% solids for capturing biotinylated biomolecules in immunoassay research, CLIA development and batch manufacturing.

What does 1% solids mean?

When expressed as w/v,1% solids corresponds to approximately10mg of magnetic beads per milliliter of suspension.

Is MSA1UM-10 supplied at the same concentration as common commercial 1µm streptavidin beads?

Several commercial1µm products are supplied at approximately10mg/mL, which is equivalent to about1% w/v. Product properties and binding capacity can still differ significantly between manufacturers.

Which molecules can MSA1UM-10 capture?

The beads can be evaluated for binding biotinylated antibodies, antigens, proteins, peptides, aptamers, DNA, RNA and other biotin-labelled molecules.

What is the binding principle?

Biotin on the ligand binds to streptavidin immobilized on the magnetic bead surface.

Does MSA1UM-10 require EDC/NHS activation?

No additional EDC/NHS bead activation is normally required for loading a properly biotinylated ligand.

Is a capture antibody included?

No. MSA1UM-10 is supplied as a streptavidin magnetic bead raw material. The customer selects and prepares the required biotinylated capture ligand.

Is the 1% suspension ready to use directly?

Not necessarily. The optimal working concentration depends on the ligand, assay volume, target concentration and instrument.

Why choose a 1µm particle size?

The1µm format can provide a practical balance between available surface, suspension behaviour, magnetic separation and automated liquid handling.

Can MSA1UM-10 be used for chemiluminescence immunoassays?

It can be evaluated as a magnetic solid phase in CLIA development. Compatibility with the complete reagent system and analyzer must be validated.

Can it be used on automated immunoassay analyzers?

It may be evaluated for automated systems. Dispensing, mixing, magnetic collection, aspiration, washing and redispersion must be validated on the specific analyzer.

How much biotinylated antibody should be loaded?

The optimal amount depends on the validated binding capacity, antibody molecular weight, degree of biotinylation and assay requirements. Several loading levels should be compared.

Does more antibody always improve assay sensitivity?

No. Excessive surface loading may cause steric crowding, reduce target accessibility and increase nonspecific binding.

Can free biotin interfere with ligand loading?

Yes. Free biotin can occupy streptavidin-binding sites and reduce binding of the intended biotinylated ligand.

How can loading efficiency be measured?

Compare the initial ligand amount with the amount remaining in the post-loading supernatant. Functional target-capture activity should also be measured.

Can non-biotinylated antibodies bind specifically to MSA1UM-10?

Non-biotinylated antibodies are not expected to bind specifically through the streptavidin–biotin interaction. Observed binding may result from nonspecific adsorption.

How should the beads be mixed?

Mix using a validated method such as inversion, roller mixing, end-over-end rotation or controlled vortexing until homogeneous.

What should be checked if magnetic collection is incomplete?

Check the magnet strength, collection time, bead concentration, buffer viscosity, aggregation and vessel geometry.

What should be checked if ligand loading is low?

Evaluate:

• Degree of biotinylation;

• Free biotin contamination;

• Ligand concentration;

• Bead quantity;

• Incubation time;

• Buffer compatibility;

• Ligand aggregation.

What should be checked if assay background is high?

Evaluate:

• Blocking formulation;

• Bead dosage;

• Ligand-loading density;

• Wash efficiency;

• Sample matrix;

• Nonspecific adsorption;

• Detection-reagent concentration.

Is MSA1UM-10 a finished diagnostic reagent?

No. MSA1UM-10 is supplied as a raw material for research and reagent development. The customer is responsible for validating the finished reagent, manufacturing process and intended application.

Are evaluation samples available?

Evaluation samples can be discussed according to the intended application, biotinylated ligand and required test quantity.

Is bulk supply available?

Pilot-scale and bulk supply can be arranged for qualified research, development and manufacturing projects.

Can the concentration, buffer or packaging be customized?

Customized solids content, streptavidin loading, suspension buffer, blocking system and packaging format may be evaluated according to project requirements.

Request a Sample or Bulk Quotation

Contact SHBC to request an evaluation sample, technical information or bulk quotation for MSA1UM-10 1µm Streptavidin Magnetic Beads.

Please provide:

• Intended immunoassay application;

• Biotinylated antibody, antigen or other ligand;

• Ligand molecular weight;

• Biotinylation method;

• Estimated degree of biotinylation;

• Required evaluation quantity;

• Estimated pilot quantity;

• Expected annual demand;

• Preferred packaging volume;

• Required technical documents;

• Required quality documents;

• Target development schedule;

• Custom specification requirements.

SHBC supports streptavidin magnetic bead projects from early ligand-loading evaluation and chemiluminescence immunoassay optimization to pilot production and bulk manufacturing.