200nm Carboxyl Red-Fluo Microspheres CFR200-10 Overview

CFR200-10 200nm Carboxyl Red-Fluo Microspheres are high-performance fluorescent polymer microspheres designed for lateral flow immunoassay (LFIA), immunochromatography reagent development, antibody conjugation, and diagnostic assay manufacturing.

With a controlled particle size of 200nm, red fluorescent optical signal, carboxyl-functionalized surface, and 1% w/v solids concentration, CFR200-10 provides a reliable fluorescent labeling material for biotechnology companies, IVD manufacturers, and research laboratories.

The surface carboxyl groups enable covalent coupling with antibodies, proteins, antigens, peptides, and other biomolecules through EDC/NHS activation chemistry.

CFR200-10 is suitable for laboratory research, fluorescent conjugate development, pilot validation, and bulk production of immunochromatography reagents.

Product Specifications of CFR200-10 Red Fluorescent Microspheres

Key Features of 200nm Carboxyl Red-Fluo Microspheres

200nm Particle Size for Optimized Immunochromatography Performance

CFR200-10 features a precisely controlled particle diameter of approximately 200nm.

Compared with smaller fluorescent nanoparticles, 200nm microspheres provide:

• Higher fluorescent dye loading capacity

• Stronger individual particle signal

• Efficient antibody attachment

• Good membrane retention

• Stable conjugate formation

The 200nm size represents a widely evaluated range for lateral flow assay development because it provides a balance between optical signal strength and migration behavior.

It is suitable for companies developing fluorescent immunochromatography platforms requiring stable and reproducible signal output.

Strong Red Fluorescence for Quantitative Detection

CFR200-10 contains red fluorescent dye integrated within the polymer particle structure.

The fluorescent signal supports:

• Quantitative lateral flow assays

• Fluorescence reader detection

• Digital immunochromatography systems

• Multiplex assay platforms

• Research diagnostic applications

Compared with conventional visual labels, fluorescence-based detection enables objective measurement and improved analytical evaluation.

Carboxyl Surface Chemistry for Antibody Coupling

The surface of CFR200-10 contains carboxyl functional groups (-COOH).

After activation with EDC/NHS chemistry, the microspheres can be covalently coupled with:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Other amine-containing biomolecules

Stable covalent conjugation helps improve reagent consistency and supports commercial assay development.

Stable Dispersion and Reliable Batch Consistency

For industrial immunoassay production, raw material consistency is critical.

CFR200-10 provides:

• Controlled particle size distribution

• Good aqueous dispersion stability

• Reliable conjugation performance

• Consistent fluorescence intensity

• Batch-to-batch reproducibility

These properties support the transition from laboratory development to commercial manufacturing.

Applications of CFR200-10 in Lateral Flow Immunoassays

Fluorescent Lateral Flow Assay Development

CFR200-10 can be used as a fluorescent reporter particle in lateral flow immunoassay development.

Typical applications include:

• Infectious disease testing research

• Biomarker detection

• Food safety analysis

• Veterinary diagnostics

• Environmental monitoring

• Pharmaceutical research

The red fluorescence signal can be detected using compatible fluorescence readers.

Antibody and Protein Conjugation

Carboxyl fluorescent microspheres provide a versatile platform for biological molecule immobilization.

Typical coupling targets:

• Detection antibodies

• Capture antibodies

• Antigens

• Proteins

• Peptides

• Nucleic acid probes

Researchers can optimize coupling density according to assay requirements.

Quantitative Immunochromatography Detection

Fluorescent lateral flow platforms allow:

• Digital signal measurement

• Quantitative analysis

• Automated reader integration

• Improved detection evaluation

CFR200-10 provides a suitable fluorescent labeling material for next-generation diagnostic platforms.

Diagnostic Reagent Manufacturing

CFR200-10 supports customers throughout the development process:

• Material selection

• Conjugate preparation

• Strip optimization

• Pilot production

• Process transfer

• Bulk manufacturing

How Do 200nm Fluorescent Microspheres Work in LFIA?

A typical fluorescent lateral flow assay includes:

Step 1: Fluorescent Conjugate Preparation

CFR200-10 microspheres are coupled with detection antibodies through carboxyl chemistry.

Step 2: Sample Application

The sample is added onto the test strip.

Step 3: Target Recognition

Target molecules bind with fluorescent antibody conjugates.

Step 4: Membrane Migration

The complexes migrate through the nitrocellulose membrane.

Step 5: Signal Capture

The target complex is captured at the test line.

Step 6: Fluorescence Detection

A fluorescence reader measures signal intensity.

Antibody Coupling Process of Carboxyl Red-Fluo Microspheres

Carboxyl Activation

CFR200-10 carboxyl groups are activated using EDC/NHS chemistry.

Important parameters:

• pH condition

• Activation time

• Activator concentration

• Particle concentration

Antibody Conjugation

Activated microspheres react with antibody amino groups.

Optimization factors:

• Protein concentration

• Particle/protein ratio

• Temperature

• Reaction time

Blocking and Purification

After coupling:

• Unreacted groups are blocked

• Free proteins are removed

• Conjugate stability is evaluated

Performance Evaluation

Recommended testing:

• Particle size

• Fluorescence intensity

• Coupling efficiency

• Dispersion stability

• Strip performance

Advantages of Fluorescent Microspheres Compared with Colloidal Gold

Quality Control and Bulk Production Capability

For IVD reagent manufacturers, consistent fluorescent microspheres are essential.

CFR200-10 quality control includes:

Particle Size Analysis

Ensures consistent migration and conjugation.

Fluorescence Performance Testing

Evaluates optical signal stability.

Surface Functional Group Testing

Confirms available carboxyl groups.

Solid Content Control

Maintains formulation accuracy.

Batch Consistency Evaluation

Supports reliable production scale-up.

Customization Options for CFR200-10

SHBC provides customized fluorescent microsphere solutions.

Particle Size Customization

Particle sizes can be optimized according to:

• Membrane characteristics

• Detection sensitivity

• Assay design

Fluorescence Customization

Available options:

• Different fluorescent colors

• Customized emission properties

• Reader compatibility

Surface Chemistry Customization

Available modifications:

• Carboxyl

• Amino

• Streptavidin

• Other functional groups

Solid Content Customization

Concentration can be adjusted according to:

• Coupling process

• Shipping requirements

• Production workflow

OEM and Bulk Manufacturing Support

SHBC supports:

• Research samples

• Pilot batches

• Bulk production

• Customized packaging

• Long-term supply

Frequently Asked Questions About CFR200-10

What are 200nm Carboxyl Red-Fluo Microspheres?

CFR200-10 are 200nm fluorescent polymer microspheres with carboxyl functional surfaces designed for lateral flow assays, immunoassay research, and antibody conjugation.

Can CFR200-10 be used for immunochromatography?

Yes. CFR200-10 is suitable for fluorescent immunochromatography reagent development and assay optimization.

How are antibodies coupled to CFR200-10?

Antibodies are commonly attached using EDC/NHS activation chemistry to form stable covalent bonds.

Why choose 200nm fluorescent microspheres?

200nm microspheres provide strong fluorescent signal, stable conjugation, and reliable assay performance.

Can CFR200-10 support bulk production?

Yes. SHBC supplies research quantities, pilot batches, and industrial-scale production.

Are customized specifications available?

Yes. Particle size, fluorescence properties, functional groups, concentration, and packaging can be customized.

Why Choose SHBC CFR200-10 Carboxyl Red-Fluo Microspheres?

SHBC specializes in fluorescent microsphere materials for immunodiagnostic research and reagent manufacturing.

CFR200-10 provides:

• 200nm controlled particle size

• Red fluorescent labeling

• Carboxyl functional surface

• Reliable antibody conjugation

• LFIA development support

• Bulk production capability

• OEM customization services

Contact SHBC for samples, technical specifications, and bulk quotation for 200nm Carboxyl Red-Fluo Microspheres CFR200-10.