200 NHS Magnetic Beads MagNHS200-10 5%

200nm NHS Magnetic Beads for Immunoassay Research

SHBC MNHS200-10 NHS Magnetic Beads are 200nm magnetic nanoparticles with an NHS-activated surface.

They are designed for direct covalent coupling of antibodies, antigens, proteins, peptides and other amine-containing biomolecules.

The product is supplied at 5% solids for immunoassay research, magnetic separation, affinity capture and bulk reagent production.

Product: 200nm NHS Magnetic Beads
Catalog Number: MNHS200-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 200nm
Surface: NHS activated
Solids Content: 5%
Main Application: Immunoassay research
Supply: Samples, pilot batches and bulk production
Use: Research use only

Product Overview

MNHS200-10 provides a ready-to-couple magnetic surface.

NHS groups react with primary amino groups on antibodies and proteins. This forms stable covalent amide bonds.

The user does not need to activate carboxyl groups with EDC and NHS before coupling.

This reduces preparation steps and simplifies magnetic reagent development.

Technical Specifications

The following data should be confirmed by the final TDS or lot-specific COA:

• Actual particle-size range

• Particle-size CV

• Magnetic material

• Magnetic content

• Magnetic response time

• NHS-group density

• Antibody coupling capacity

• Protein coupling capacity

• Suspension buffer

• Preservative

• Storage temperature

• Package size

• Shelf life

Key Advantages

Ready-to-Couple Surface

The NHS surface is already activated.

No additional EDC/NHS activation step is normally required.

Simple Antibody Coupling

Mix the beads with an antibody in a compatible amine-free buffer.

The antibody can react directly with the NHS surface.

Stable Covalent Bond

NHS groups react with primary amines to form stable amide linkages.

The coupled ligand is resistant to loss during repeated washing.

200nm Particle Size

The nanoscale particles provide a high particle number and large collective surface area.

This can support rapid suspension-phase interaction and small reaction volumes.

Magnetic Separation

The beads can be collected with an external magnetic field.

This simplifies washing, buffer exchange and removal of unbound reagents.

Suitable for Automation

The magnetic format can be evaluated in manual, semi-automated and automated immunoassay workflows.

Actual magnetic collection and redispersion must be validated on the intended instrument.

5% Solids Content

The concentrated suspension supports laboratory dilution, pilot coupling and production-scale reagent preparation.

Bulk Supply

SHBC supports research samples, pilot lots, repeated orders, OEM projects and enterprise bulk purchasing.

How NHS Coupling Works

NHS esters react with primary amino groups on biomolecules.

Primary amino groups are commonly present on:

• Lysine residues

• Protein N-termini

• Peptide N-termini

• Amino-modified oligonucleotides

• Amino-modified synthetic ligands

The simplified reaction is:

NHS Magnetic Bead + Amine-Containing Ligand → Covalently Coupled Bead–Ligand

The coupling reaction should use an amine-free buffer.

Avoid Tris, glycine and other primary amine-containing components during coupling because they may consume NHS groups and reduce ligand loading.

NHS groups are also sensitive to hydrolysis. Prepare and use the beads according to the validated coupling procedure.

Immunoassay Applications

Magnetic Sandwich Immunoassays

Couple a capture antibody to MNHS200-10.

The antibody-coated beads can capture a target antigen from the sample.

A labeled detection antibody is then added to generate the assay signal.

Chemiluminescent Immunoassay Research

MNHS200-10 can be evaluated as a magnetic solid phase in CLIA and CLEIA reagent development.

Potential targets include:

• Hormones

• Cytokines

• Tumor biomarkers

• Cardiac biomarkers

• Inflammatory markers

• Infectious-disease antigens

• Autoantibodies

• Veterinary biomarkers

Competitive Immunoassays

NHS magnetic beads may be used for small molecules, haptens, drugs, hormones, toxins and other targets suitable for competitive assay formats.

Antigen-Coated Magnetic Beads

Recombinant antigens and proteins can be immobilized for:

• Antibody detection

• Serological assay research

• Autoantibody research

• Vaccine-response studies

• Antibody screening

Affinity Capture

Antibody- or protein-coated beads can be used for:

• Target enrichment

• Immunoprecipitation research

• Protein purification research

• Antigen capture

• Protein interaction studies

Automated Magnetic Washing

The magnetic solid phase supports:

• Sample incubation

• Magnetic capture

• Supernatant removal

• Repeated washing

• Reporter incubation

• Final signal detection

Suitable Coupling Molecules

MNHS200-10 may be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Lectins

• Receptors

• Amino-modified oligonucleotides

• Amino-modified aptamers

• Amine-containing synthetic ligands

The ligand must contain an accessible primary amino group.

Recommended Coupling Workflow

1. Resuspend the Beads

Mix MNHS200-10 until the suspension is homogeneous.

Avoid excessive foaming.

2. Magnetically Collect the Beads

Place the tube in a compatible magnetic separator.

Remove the storage solution after the beads have collected.

3. Wash with Coupling Buffer

Use a validated amine-free buffer.

Do not use Tris or glycine during the coupling reaction.

4. Prepare the Ligand

Transfer the antibody or protein into the coupling buffer.

Remove incompatible stabilizers or free amines when necessary.

5. Add the Ligand

Mix the ligand with the NHS magnetic beads immediately after bead preparation.

6. Incubate

Maintain gentle mixing during coupling.

Optimize:

• Ligand concentration

• Bead concentration

• Buffer pH

• Reaction time

• Temperature

• Mixing speed

7. Wash the Coupled Beads

Magnetically collect the beads.

Remove unbound ligand with a suitable wash buffer.

8. Quench Remaining NHS Groups

Use an application-compatible quenching reagent.

This helps reduce unwanted surface reactions and assay background.

9. Block and Formulate

Add a suitable blocker and storage formulation.

Evaluate dispersion, magnetic recovery, ligand activity and stability.

Magnetic Immunoassay Workflow

1. Couple the capture antibody to MNHS200-10.

2. Quench and block the coupled beads.

3. Add the sample.

4. Incubate to capture the target.

5. Magnetically separate and wash.

6. Add the labeled detection antibody.

7. Incubate again.

8. Perform final magnetic washing.

9. Add the detection substrate.

10. Measure the signal.

Possible detection formats include:

• Chemiluminescence

• Fluorescence

• Enzyme colorimetry

• Electrochemiluminescence

• Other compatible reporter systems

NHS Magnetic Beads vs Carboxyl Magnetic Beads

Choose NHS magnetic beads when a shorter direct-coupling workflow is preferred.

Choose carboxyl magnetic beads when the user already has a validated EDC/NHS process.

Quality Control

Recommended quality-control items include:

• Particle diameter

• Particle-size CV

• Suspension appearance

• Solids content

• NHS activity

• Magnetic response

• Magnetic recovery

• Redispersion

• Antibody coupling capacity

• Functional target binding

• Non-specific binding

• Aggregate level

• Batch consistency

Coupling Efficiency

Coupling efficiency can be estimated by measuring:

• Initial protein concentration

• Protein remaining after coupling

• Protein in wash solutions

Protein uptake does not equal functional antibody activity.

Always confirm performance with a target-binding immunoassay.

Functional Testing

A model immunoassay may compare:

• Positive signal

• Negative background

• Signal-to-background ratio

• Precision

• Recovery

• Magnetic washing efficiency

• Lot-to-lot consistency

Bulk Manufacturing and Customization

Shanghai SanYu Biotechnology Co., Ltd. supports:

• Research samples

• Pilot batches

• Bulk production

• OEM supply

• Private-label packaging

• Custom catalog numbers

• Custom package sizes

• Custom solids concentration

• Custom NHS-group density

• Custom magnetic response

• Customer-specific quality standards

For project evaluation, provide:

• Required quantity

• Annual demand

• Intended immunoassay

• Ligand type

• Required coupling capacity

• Magnetic separator or analyzer

• Preferred package size

• Buffer restrictions

• Preservative restrictions

• Quality-control requirements

Handling and Storage

Follow the final SHBC product label, TDS and lot-specific COA.

General recommendations:

• Store under the specified refrigerated conditions.

• Do not freeze unless validated.

• Keep the container tightly closed.

• Mix before sampling.

• Do not allow the beads to dry.

• Use clean, low-binding tubes.

• Protect NHS groups from moisture and hydrolysis.

• Avoid amine-containing buffers before coupling.

• Use prepared beads promptly according to the validated procedure.

Frequently Asked Questions

What is MNHS200-10?

MNHS200-10 is a 200nm NHS-activated magnetic bead suspension supplied at 5% solids.

What is the main application?

It is designed for antibody and protein coupling in immunoassay research.

Is EDC/NHS activation required?

No additional EDC/NHS activation is normally required because the surface is already NHS activated.

Which groups react with NHS?

NHS esters mainly react with primary amino groups.

Can antibodies be coupled?

Yes. Antibodies containing accessible primary amino groups can be covalently immobilized.

Can antigens and proteins be coupled?

Yes. Recombinant antigens, proteins, peptides and enzymes may be evaluated.

Which buffers should be avoided?

Avoid Tris, glycine and other primary amine-containing buffers during coupling.

Is quenching required?

Quenching remaining NHS groups is generally recommended after ligand coupling.

Can the beads be used in CLIA research?

Yes. They can be evaluated as a magnetic solid phase in chemiluminescent immunoassay development.

Can the product be used on automated analyzers?

Yes, subject to validation of magnetic response, dispensing, washing and redispersion on the intended instrument.

What is the antibody coupling capacity?

Use the final product specification or lot-specific COA. Do not estimate it from solids content alone.

How are the beads separated?

Use an external magnetic separator compatible with the vessel and sample volume.

Can the beads be frozen?

Freezing is generally not recommended unless validated.

Is bulk production available?

Yes. SHBC supports samples, pilot batches, OEM projects and bulk manufacturing.

Request a Sample or Quotation

SHBC MNHS200-10 provides:

• 200nm nominal particle size

• NHS-activated surface

• 5% solids content

• Direct amine-based coupling

• Stable covalent ligand attachment

• Magnetic separation

• Immunoassay compatibility

• Sample and bulk supply

• OEM and customization support

Product Name: 200nm NHS Magnetic Beads
Catalog Number: MNHS200-10
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Particle Size: 200nm
Surface: NHS activated
Solids Content: 5%
Application: Immunoassay research
Supply: Samples, pilot batches and bulk production
Intended Use: Research Use Only. Not for diagnostic or therapeutic use.