200nm Carboxyl Green-Fluo Microspheres CFG200-10 1%

200nm Carboxyl Green-Fluo Microspheres CFG200-10

SHBC CFG200-10 are 200 nm carboxyl-functionalized green fluorescent microspheres developed for fluorescent immunoassays, lateral flow tests, biosensors and biomolecule conjugation.

The particles have 470/488 nm excitation, 525 nm emission and 1% solid content. Reactive carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size, particle size distribution, fluorescence intensity and carboxyl content are available in the Certificate of Analysis.

Green Fluorescence at 470/488–525nm

CFG200-10 can be excited by approximately 470 nm or 488 nm light and emits green fluorescence near 525 nm.

The optical profile is suitable for compatible fluorescence readers, imaging systems and green-channel detection instruments.

Key Advantages

Bright Green Fluorescence

The 525 nm emission provides a clear signal for qualitative and quantitative fluorescence detection.

Uniform 200nm Particle Size

Controlled particle sizing supports repeatable membrane migration, protein coupling and assay performance.

Reactive Carboxyl Surface

Surface COOH groups enable covalent immobilization of antibodies, antigens, proteins and other amino-containing molecules.

High Signal Capacity

The 200 nm particle size provides greater fluorescent loading potential than smaller nanoparticles.

Efficient Biomolecule Loading

Each particle provides a useful surface area for antibody and protein immobilization.

R&D and Bulk Production

SHBC supports laboratory samples, pilot validation, customized specifications and industrial batch supply.

Main Applications

Fluorescent Lateral Flow Assays

CFG200-10 can be evaluated as a fluorescent reporter for qualitative and quantitative lateral flow tests.

Fluorescence Immunoassays

Suitable for sandwich, competitive and indirect fluorescent immunoassay formats.

Antibody and Antigen Conjugation

The carboxyl surface supports covalent immobilization of antibodies, antigens and recombinant proteins.

Biosensor Development

Can be used as a fluorescent signal carrier in optical biosensors and surface-based detection platforms.

Protein and Peptide Labeling

Suitable for coupling proteins, peptides, enzymes and other amino-containing biomolecules.

Microfluidic Detection

The particles can be evaluated in compatible microfluidic channels, biochips and fluorescence detection systems.

Diagnostic Reagent Development

Suitable for medical, veterinary, food-safety and environmental assay research and production.

Benefits for Fluorescent Immunoassays

CFG200-10 provides:

• Green fluorescence near 525 nm

• Compatibility with 488 nm excitation

• Reactive COOH coupling sites

• Strong accumulated assay signal

• Good surface area for protein loading

• Flexible biomolecule modification

• Sample and bulk availability

• Custom manufacturing support

Biomolecule Coupling

Surface carboxyl groups can be activated using EDC or EDC/NHS chemistry and reacted with primary amine groups on antibodies or proteins.

A general coupling workflow includes:

1. Redisperse and wash the microspheres.

2. Activate the surface carboxyl groups.

3. Add the antibody or target protein.

4. Block remaining reactive sites.

5. Wash and resuspend the conjugate.

6. Evaluate coupling efficiency and fluorescence performance.

Coupling pH, protein concentration, activation time and reagent ratio should be optimized for each biomolecule.

LFIA Development Considerations

For fluorescent lateral flow assays, evaluate:

• Antibody-to-particle ratio

• Microsphere concentration

• Carboxyl group density

• Nitrocellulose membrane type

• Conjugate pad release

• Running buffer composition

• Surfactant concentration

• Test-line capture density

• Background fluorescence

• Reader filter settings

The formulation should balance fluorescence signal, membrane migration and low background.

200nm Particle Size Benefits

Compared with smaller fluorescent nanoparticles, 200 nm microspheres may provide:

• Higher fluorescence per particle

• Larger coupling area per particle

• Improved test-line signal accumulation

• Easier purification and recovery

• Better optical detection

• Greater ligand-loading capacity

Actual assay sensitivity should be confirmed using the selected membrane, antibody pair and fluorescence reader.

Assay Optimization

Important development parameters include:

• Ligand loading

• Coupling efficiency

• Blocking reagent

• Particle aggregation

• Conjugate stability

• Membrane migration rate

• Test-line intensity

• Non-specific adsorption

• Reader sensitivity

• Batch consistency

Small-scale optimization is recommended before production scale-up.

Dispersion and Handling

Before use:

• Mix gently before sampling

• Redisperse settled particles completely

• Use controlled bath sonication when required

• Avoid excessive bubbles and foam

• Protect the suspension from strong light

• Prevent the particles from drying

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

High salt concentration, unsuitable solvents or incorrect pH may reduce dispersion stability.

Purification and Washing

Suitable purification methods may include:

• Centrifugation

• Ultrafiltration

• Tangential flow filtration

• Size-exclusion chromatography

• Dialysis

Centrifugation speed and time should be optimized to achieve particle recovery without forming a difficult-to-disperse pellet.

Instrument Compatibility

Use fluorescence instruments with optical settings compatible with:

• Excitation near 470 or 488 nm

• Emission detection near 525 nm

• Suitable green-channel filters

• Appropriate detector sensitivity

For LFIA development, confirm that the reader can measure the required test-line signal range.

Custom Carboxyl Green-Fluo Microspheres

SHBC can customize fluorescent microspheres according to assay and production requirements.

Customization options may include:

• Custom particle size

• Custom fluorescence wavelength

• Different fluorescence colors

• Carboxyl group density

• Fluorescence intensity

• Solid content

• Dispersion buffer

• Surface modification

• Biomolecule conjugation

• Custom packaging

• OEM and ODM production

Please provide the target application, biomolecule, detection instrument and required quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Dispersion stability data

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.

SHBC provides:

• Controlled microsphere manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFG200-10?

CFG200-10 is a 200 nm carboxyl-functionalized green fluorescent microsphere suspension with 1% solid content.

What are the excitation and emission wavelengths?

The excitation wavelengths are 470/488 nm, and the emission wavelength is 525 nm.

What is CFG200-10 mainly used for?

It is suitable for fluorescent immunoassays, lateral flow tests, biosensors, biomolecule coupling and diagnostic reagent development.

Can antibodies be coupled to CFG200-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling chemistry can be used?

EDC or EDC/NHS chemistry can be evaluated for activating the surface carboxyl groups.

Can CFG200-10 be excited with a 488nm laser?

Yes. The specified excitation range includes 488 nm, with green emission near 525 nm.

Is CFG200-10 suitable for lateral flow assays?

Yes. Membrane type, antibody loading, conjugate release, running buffer and fluorescence reader settings should be optimized.

How does 200nm compare with 60nm particles?

The 200 nm particles may provide higher fluorescence and more coupling area per particle. The 60 nm particles may provide faster diffusion and membrane migration.

Can CFG200-10 be separated by centrifugation?

Centrifugation can be evaluated, but speed and time should be optimized according to the buffer, particle concentration and required recovery.

How should the microspheres be dispersed?

Use gentle mixing or controlled bath sonication while avoiding excessive foam, particle drying and incompatible buffers.

Can CFG200-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer and packaging can be customized.

Are samples and bulk quantities available?

Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.

Request a Sample or Quotation

Contact SHBC for CFG200-10 samples, technical specifications, coupling support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Assay format

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 200nm Carboxyl Green-Fluo Microspheres
Catalog Number: CFG200-10
Particle Size: 200 nm
Solid Content: 1%
Excitation/Emission: 470/488–525 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.