200nm Carboxyl Orange-Fluo Microspheres 1% CFO200-10

200nm Carboxyl Orange-Fluo Microspheres CFO200-10

SHBC CFO200-10 is a 200nm carboxyl functionalized orange fluorescent microsphere designed for biomolecule labeling, immunoassay development and fluorescence-based biological detection.

The microspheres provide stable orange fluorescence with an excitation wavelength of 465nm and an emission wavelength of 585nm.

With reactive carboxyl groups (-COOH) on the surface, CFO200-10 enables covalent coupling with antibodies, proteins, peptides and other biological molecules through EDC/NHS chemistry.

Product Specifications of CFO200-10

Orange Fluorescence Properties (Ex 465nm / Em 585nm)

CFO200-10 provides bright and stable orange fluorescence for fluorescence-based detection applications.

Optical characteristics:

• Excitation wavelength: 465nm

• Emission wavelength: 585nm

• Fluorescence color: Orange

The defined fluorescence spectrum enables compatibility with fluorescence readers, imaging systems and analytical platforms.

Stable fluorescence performance supports reliable signal detection and consistent assay results.

Carboxyl Functionalized Microspheres for Biomolecule Coupling

CFO200-10 contains surface carboxyl groups that provide active sites for covalent biomolecule immobilization.

The carboxyl groups can be activated using EDC/NHS chemistry to react with amino groups of proteins and antibodies.

Typical coupling workflow:

Carboxyl Microsphere Activation → Biomolecule Coupling → Blocking → Fluorescent Conjugate Formation

Advantages:

• Strong covalent attachment

• Improved conjugate stability

• Reduced biomolecule loss

• Flexible surface modification

Advantages of 200nm Orange Fluorescent Beads

Controlled 200nm Particle Size

The 200nm particle size provides:

• High surface area

• Efficient biomolecule loading

• Good dispersion stability

• Suitable fluorescence performance

• Reliable assay reproducibility

Reactive Carboxyl Surface

The -COOH functional groups support:

• Antibody immobilization

• Protein coupling

• Peptide conjugation

• Diagnostic reagent development

• Custom surface modification

Stable Orange Fluorescence

CFO200-10 provides:

• Stable emission signal

• Consistent fluorescence intensity

• Good batch uniformity

• Compatibility with fluorescence detection systems

Applications in Immunoassay and Fluorescence Detection

CFO200-10 can be used as a fluorescent carrier for biological detection platforms.

Potential applications include:

• Immunofluorescence assays

• Antibody labeling

• Antigen detection

• Biomarker analysis

• Diagnostic reagent development

• Research assay optimization

The combination of controlled particle size, orange fluorescence and carboxyl functionality makes CFO200-10 suitable for advanced bioassay development.

Antibody and Protein Conjugation Using Carboxyl Microspheres

Carboxyl fluorescent microspheres are commonly used for covalent coupling of biological molecules.

Compatible molecules include:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Recombinant proteins

• Peptides

• Nucleic acids

General conjugation process:

1. Microsphere washing

2. Carboxyl group activation with EDC/NHS

3. Biomolecule addition

4. Covalent coupling reaction

5. Blocking residual active sites

6. Conjugate performance testing

The coupling conditions can be optimized according to different assay requirements.

Fluorescent Microspheres for Lateral Flow Assay Development

CFO200-10 can be evaluated as an orange fluorescent labeling material for lateral flow assay applications.

Potential benefits:

• Enhanced detection sensitivity

• Stable fluorescence signal

• Flexible antibody conjugation

• Compatibility with fluorescence readers

Applications include:

• POCT assay development

• Infectious disease detection

• Clinical biomarker testing

• Food safety analysis

• Veterinary diagnostics

Orange Fluorescent Beads for Flow Cytometry and Bioanalysis

CFO200-10 fluorescent microspheres may be used in fluorescence analytical platforms.

Potential applications:

• Flow cytometry calibration

• Fluorescent particle standards

• Multiplex detection systems

• Bioanalytical research

The defined orange fluorescence wavelength helps support reliable optical detection.

Comparison of CFO200-10 Carboxyl Fluorescent Microspheres

Compared with non-functional fluorescent particles, carboxyl fluorescent microspheres provide additional flexibility for antibody and protein immobilization.

Storage and Handling Recommendations

For maintaining optimal performance:

• Avoid repeated freeze-thaw cycles

• Protect from direct light exposure

• Mix gently before use

• Maintain uniform particle suspension

• Store according to recommended conditions

Proper handling helps maintain fluorescence stability and particle consistency.

Custom Fluorescent Microsphere Manufacturing

Shanghai SanYu Biotechnology Co., Ltd. provides customized fluorescent microsphere solutions for research and industrial applications.

Customization options include:

• Particle size selection

• Fluorescence color customization

• Surface functional groups

• Solid content adjustment

• Bulk manufacturing supply

Available products include:

• Carboxyl fluorescent microspheres

• Amino fluorescent microspheres

• Magnetic fluorescent beads

• Standard fluorescent particles

Frequently Asked Questions

What are CFO200-10 microspheres?

CFO200-10 are 200nm carboxyl functionalized orange fluorescent microspheres designed for biomolecule coupling and fluorescence detection.

What are the excitation and emission wavelengths?

The excitation wavelength is 465nm and emission wavelength is 585nm.

What surface functional group does CFO200-10 contain?

CFO200-10 contains reactive carboxyl (-COOH) groups.

Can CFO200-10 be used for antibody conjugation?

Yes. The carboxyl groups support antibody and protein coupling through EDC/NHS activation chemistry.

What applications use orange fluorescent microspheres?

They are commonly used in immunoassays, fluorescence labeling, lateral flow assays and bioanalysis.

What is the solid content?

The standard solid content is 1%.

Does SHBC provide bulk fluorescent microspheres?

Yes. SHBC provides laboratory, pilot-scale and industrial bulk supply of fluorescent microspheres.

Request CFO200-10 Samples and Bulk Pricing

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical information and bulk quotations of 200nm Carboxyl Orange-Fluo Microspheres CFO200-10.

CFO200-10 combines controlled 200nm particle size, stable orange fluorescence and carboxyl surface chemistry for advanced immunoassay and fluorescence detection applications.