200nm Carboxyl Yellow-Fluo Microspheres 1% CFY200-10

200nm Carboxyl Yellow-Fluo Microspheres CFY200-10

SHBC CFY200-10 is a carboxyl functionalized yellow fluorescent microsphere with an average particle size of 200nm and 1% solid content.

The microspheres provide stable yellow fluorescence with excitation wavelength at 465nm and emission wavelength at 510nm.

With surface carboxyl groups (-COOH), CFY200-10 enables covalent coupling with proteins, antibodies, peptides, and other biomolecules, making it suitable for immunoassay development, fluorescence labeling, and bioanalytical applications.

Product Specifications of CFY200-10

Carboxyl Functionalized Fluorescent Microsphere Surface Chemistry

CFY200-10 contains surface carboxyl groups that provide reactive sites for biomolecule immobilization.

The carboxyl groups can be activated using carbodiimide chemistry such as EDC/NHS coupling.

Typical conjugation process:

Carboxyl Microsphere Activation → EDC/NHS Activation → Antibody or Protein Coupling → Fluorescent Bioconjugate Formation

The covalent coupling method provides:

• Stable biomolecule attachment

• Reduced antibody leakage

• Improved assay reproducibility

• Flexible surface modification options

Yellow Fluorescence Characteristics (Ex 465nm / Em 510nm)

CFY200-10 provides bright yellow fluorescence suitable for fluorescence-based detection platforms.

Optical properties:

• Excitation wavelength: 465nm

• Emission wavelength: 510nm

The fluorescence signal can be detected using compatible fluorescence readers, imaging systems, or analytical instruments.

Stable fluorescence performance supports applications requiring consistent signal intensity and reliable detection.

Key Advantages of CFY200 Yellow Fluorescent Beads

200nm Uniform Particle Size

The 200nm particle size provides:

• High surface area

• Efficient biomolecule loading

• Good dispersion stability

• Suitable optical properties

• Reliable assay performance

Carboxyl Surface Modification

The -COOH functional groups allow:

• Antibody conjugation

• Protein immobilization

• Peptide coupling

• Nucleic acid attachment

• Custom surface modification

Stable Yellow Fluorescence

Advantages include:

• Strong fluorescence signal

• Stable emission performance

• Good batch consistency

• Compatibility with fluorescence detection systems

Applications in Immunoassay and Bioanalysis

CFY200-10 can be used for development of fluorescence-based biological detection systems.

Potential applications include:

• Immunofluorescence assays

• Fluorescent antibody labeling

• Protein detection

• Biomarker analysis

• Diagnostic reagent development

• Research assay development

The carboxyl surface enables covalent attachment of antibodies or proteins for specific target recognition.

Microsphere-Based Biomolecule Conjugation

Carboxyl fluorescent microspheres are widely used as carriers for biomolecule immobilization.

Typical coupling molecules include:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Proteins

• Peptides

• DNA/RNA molecules

Common conjugation workflow:

1. Wash fluorescent microspheres

2. Activate carboxyl groups using EDC/NHS

3. Add biomolecule containing amino groups

4. Form stable amide bonds

5. Block remaining active sites

6. Evaluate conjugate performance

Fluorescent Microspheres for Lateral Flow Assay Development

Yellow fluorescent microspheres can be evaluated as labeling materials for fluorescence-based lateral flow platforms.

Potential benefits:

• Enhanced detection sensitivity

• Stable fluorescent signal

• Flexible antibody coupling

• Suitable for POCT development

• Compatible with fluorescence readers

Applications include:

• Infectious disease testing

• Biomarker detection

• Veterinary diagnostics

• Food safety testing

• Environmental analysis

Fluorescent Beads for Flow Cytometry and Multiplex Detection

CFY200-10 fluorescent microspheres may be evaluated as calibration particles or assay carriers for fluorescence detection platforms.

Potential applications:

• Flow cytometry calibration

• Fluorescent bead standards

• Multiplex assay development

• Particle-based detection systems

The defined fluorescence wavelength helps support instrument compatibility and signal differentiation.

Advantages Compared with Non-Functional Fluorescent Microspheres

Compared with non-functional fluorescent beads, carboxyl fluorescent microspheres provide additional flexibility for biomolecule immobilization and assay development.

Storage and Handling Recommendations

For optimal performance:

• Avoid repeated freeze-thaw cycles

• Maintain uniform dispersion before use

• Store under recommended conditions

• Protect from strong light exposure

• Mix gently before sampling

Proper handling helps maintain particle size distribution and fluorescence stability.

Customization and Bulk Supply Options

Shanghai SanYu Biotechnology Co., Ltd. provides:

• Different particle sizes

• Different fluorescence colors

• Customized surface groups

• Bulk production capability

• OEM and research supply services

Available functional microsphere options include:

• Carboxyl fluorescent microspheres

• Amino fluorescent microspheres

• Standard fluorescent beads

• Magnetic fluorescent microspheres

Frequently Asked Questions

What are CFY200-10 microspheres?

CFY200-10 are 200nm carboxyl functionalized yellow fluorescent microspheres designed for biomolecule coupling and fluorescence detection applications.

What are the excitation and emission wavelengths?

The excitation wavelength is 465nm and the emission wavelength is 510nm.

What surface group does CFY200-10 have?

CFY200-10 contains carboxyl (-COOH) functional groups for covalent coupling.

Can CFY200-10 be conjugated with antibodies?

Yes. The carboxyl groups can be activated using EDC/NHS chemistry for antibody and protein coupling.

What applications use yellow fluorescent microspheres?

They are commonly evaluated for immunoassays, fluorescence labeling, lateral flow assays, and bioanalysis.

What is the solid content?

The solid content of CFY200-10 is 1%.

Can SHBC provide bulk quantities?

Yes. SHBC provides laboratory, pilot-scale, and bulk supply of fluorescent microspheres.

Request CFY200-10 Samples and Bulk Pricing

Contact Shanghai SanYu Biotechnology Co., Ltd. for technical information, samples, and bulk quotations of 200nm Carboxyl Yellow-Fluo Microspheres.

CFY200-10 provides a combination of controlled particle size, stable yellow fluorescence, and carboxyl surface chemistry for advanced biological detection and assay development.