200 um Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB200UM 10%

200 μm Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB200UM

SHBC CPS/DVB200UM Carboxyl Crosslinked PS/DVB Microspheres are large 200 μm functional polymer beads featuring a crosslinked polystyrene/divinylbenzene matrix and reactive surface carboxyl groups.

Supplied at 10% solids, CPS/DVB200UM is designed for antibody coupling, protein immobilization, biomolecule capture, large-bead immunoassay research and custom functional particle development.

The 200 μm particle format provides straightforward handling, rapid settling and convenient recovery for laboratory research and scalable batch processing.

CPS/DVB200UM Technical Specifications

Crosslinked PS/DVB Polymer Matrix

CPS/DVB200UM uses divinylbenzene to form a crosslinked polystyrene network.

Crosslinking increases the rigidity and structural stability of large polystyrene particles. Commercial suppliers offer crosslinked PS microspheres in the approximately 175–210 μm and 200–600 μm size ranges for large-particle applications.

Key benefits include:

• Rigid polymer structure

• Stable spherical particle format

• Good mechanical handling

• Convenient repeated washing

• Easy particle recovery

• Suitable for surface functionalization

• Suitable for batch processing

Carboxyl Surface for Covalent Biomolecule Coupling

The particle surface contains carboxyl (-COOH) groups that can be activated for covalent coupling with amine-containing biomolecules.

Typical coupling route:

Carboxyl Surface → EDC/NHS Activation → Amine-Containing Biomolecule → Amide Bond

Carboxyl-functional polystyrene and PS/DVB microspheres are established substrates for carbodiimide-mediated attachment of proteins and other amine-containing molecules.

Key Advantages of CPS/DVB200UM

• Large 200 μm particle size

• High 10% solid content

• Crosslinked PS/DVB matrix

• Reactive carboxyl surface

• Suitable for EDC/NHS coupling

• Stable covalent ligand immobilization

• Rapid particle settling

• Easy washing and recovery

• Suitable for batch biomolecule coupling

• Suitable for research and bulk production

Why Choose 200 μm Carboxyl Microspheres?

The 200 μm particle format is especially suitable when easy physical recovery and large-particle handling are more important than maximizing surface area per unit mass.

Potential advantages include:

• Rapid sedimentation

• Easy visual observation

• Convenient centrifugation

• Easy filtration or sieving where appropriate

• Straightforward washing

• Reduced loss during liquid exchange

• Convenient batch coupling

• Suitable for large-bead assay formats

• Easy scale-up of particle handling

Large crosslinked PS beads in the 200 μm range are already commercially used for protein-related and bead-bed applications, demonstrating the usefulness of this particle size class.

Immunoassay Applications

Antibody-Coupled Large Microspheres

Capture antibodies can be covalently immobilized on CPS/DVB200UM for research applications such as:

• Heterogeneous bead-based immunoassays

• Antigen capture studies

• Biomarker-binding research

• Serological assay development

• Antibody-antigen interaction studies

• Custom diagnostic reagent development

Because 200 μm particles are much larger than conventional latex immunoassay particles, they are better suited to large-bead heterogeneous assay formats than homogeneous turbidimetric assays.

Antigen-Coupled Microspheres

Diagnostic or research antigens can also be immobilized for:

• Antibody detection

• Serology research

• Autoantibody research

• Infectious-disease assay development

• Antibody-binding studies

• Assay optimization

Protein and Biomolecule Immobilization

CPS/DVB200UM can also be evaluated for:

• Protein immobilization

• Enzyme immobilization

• Peptide coupling

• Affinity ligand preparation

• Biomolecule capture

• Biosensor development

• Surface chemistry research

• Amino-modified oligonucleotide coupling

• Custom functional bead preparation

Suitable Coupling Molecules

The carboxyl-functional surface can be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Diagnostic antigens

• Peptides

• Enzymes

• Amino-modified DNA

• Amino-modified RNA

• Other amine-containing ligands

Final coupling efficiency depends on ligand properties and optimized reaction conditions.

General EDC/NHS Coupling Workflow

1. Resuspend CPS/DVB200UM

Mix the microspheres thoroughly before sampling.

Because 200 μm particles settle rapidly, resuspend immediately before transferring the suspension.

2. Wash the Microspheres

Wash with a suitable activation buffer.

Large particles are generally easy to recover during washing.

3. Activate Surface Carboxyl Groups

Add EDC, with or without NHS, to activate the surface -COOH groups.

4. Add the Biomolecule

Add purified antibody, antigen, protein or another amine-containing ligand.

5. Perform Covalent Coupling

Allow activated carboxyl groups to react with primary amines on the biomolecule.

6. Block Residual Reactive Sites

Use a suitable quenching or blocking reagent.

7. Wash and Resuspend

Remove unbound ligand and coupling reagents, then resuspend the functionalized particles in the selected assay or storage buffer.

EDAC/EDC-mediated coupling is a commonly used strategy for covalent immobilization of amine-containing molecules on carboxyl polymer microspheres.

Factors Affecting Coupling Efficiency

Important optimization factors include:

• Microsphere concentration

• Protein concentration

• EDC concentration

• NHS concentration

• Activation pH

• Coupling-buffer composition

• Reaction temperature

• Reaction time

• Mixing method

• Blocking conditions

Small-scale coupling studies are recommended before transferring the process to bulk production.

Handling Tips for 200 μm Microspheres

Large microspheres behave differently from nano- and small-micron particles.

Recommended handling practices include:

• Mix before every sampling step.

• Maintain gentle agitation during coupling.

• Prevent prolonged settling.

• Use wide-bore pipette tips when necessary.

• Avoid unnecessarily narrow tubing.

• Minimize transfer steps.

• Confirm particle recovery after washing.

• Avoid aggressive mechanical agitation.

• Validate mixing during process scale-up.

CPS/DVB200UM vs. Smaller Carboxyl Microspheres

Smaller particles generally provide greater surface area per unit mass, while 200 μm particles prioritize handling and recovery.

200 μm Carboxyl PS/DVB vs. Standard Large PS Beads

Commercial 200 μm-scale crosslinked PS beads are widely available, but many standard large-particle products are supplied without reactive surface functionalization.

Covalent Coupling vs. Passive Protein Adsorption

Quality Control for Bulk Production

For research and immunoassay reagent development, recommended evaluation parameters include:

• Particle-size consistency

• Particle morphology

• Solid-content consistency

• Surface carboxyl functionality

• Suspension behavior

• Coupling efficiency

• Ligand loading

• Recovery after washing

• Nonspecific binding

• Assay signal

• Storage stability

• Batch-to-batch consistency

Why Choose SHBC CPS/DVB200UM?

• 200 μm carboxyl crosslinked PS/DVB microspheres

• High 10% solid-content suspension

• Reactive COOH surface

• Suitable for EDC/NHS chemistry

• Designed for antibody and protein immobilization

• Easy large-particle recovery

• Suitable for batch coupling

• Suitable for immunoassay research

• Sample evaluation available

• Bulk manufacturing capability

• Custom quantity and packaging support

Storage and Handling

Recommended practices include:

• Keep the container tightly sealed.

• Mix thoroughly before sampling.

• Resuspend settled particles before use.

• Avoid unnecessary freezing.

• Prevent microbial contamination.

• Use suitable pipette tips for large particles.

• Avoid conditions causing irreversible aggregation.

• Validate storage conditions after biomolecule coupling.

Frequently Asked Questions

What is CPS/DVB200UM?

CPS/DVB200UM is a 200 μm carboxyl-functional crosslinked polystyrene/divinylbenzene microsphere product supplied at 10% solids.

What does PS/DVB mean?

PS/DVB means polystyrene crosslinked with divinylbenzene.

Why use DVB-crosslinked polystyrene?

Crosslinking creates a more rigid and structurally stable polymer particle, which is especially useful for large microsphere formats.

What is the purpose of the carboxyl surface?

Carboxyl groups provide reactive sites for covalent attachment of antibodies, proteins and other amine-containing molecules.

Can antibodies be coupled to CPS/DVB200UM?

Yes. Antibodies can be immobilized using optimized EDC or EDC/NHS chemistry.

Can CPS/DVB200UM be used for immunoassays?

Yes. It is primarily suited to large-bead heterogeneous immunoassay research, antigen capture and biomolecule-binding studies.

Is CPS/DVB200UM suitable for latex turbidimetric immunoassays?

200 μm particles are much larger than typical latex particles used in homogeneous turbidimetric assays. Smaller microspheres are generally more appropriate for PETIA and latex agglutination formats.

Can CPS/DVB200UM be used in flow cytometry?

200 μm particles are outside the typical particle range of most conventional flow cytometers. Instrument compatibility should be confirmed before use.

What is the solid content?

CPS/DVB200UM is supplied at 10% solids.

Can amino-modified DNA or RNA be coupled?

Yes. Amino-modified oligonucleotides may be evaluated for carbodiimide-mediated coupling to the carboxyl surface.

Why choose 200 μm instead of smaller microspheres?

200 μm particles provide very easy recovery, washing and physical handling. Smaller particles provide substantially greater surface area per unit mass.

Do 200 μm microspheres settle quickly?

Yes. Regular resuspension or gentle mixing is recommended during coupling and assay procedures.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk production for biotechnology companies, immunoassay developers and research organizations.

Request CPS/DVB200UM Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for CPS/DVB200UM samples, technical documentation and bulk quotations.

Please provide:

• Target antibody or protein

• Intended application

• Coupling chemistry

• Required quantity

• Assay format

• Development stage

• Estimated annual demand

CPS/DVB200UM is supplied for research and process development. Final diagnostic applications should be validated by the customer.