20um Nickel Magnetic Beads MNi20UM-10 5%

20um Nickel Magnetic Beads MNi20UM-10

SHBC MNi20UM-10 Nickel Magnetic Beads are 20 μm magnetic microspheres with a nickel-affinity surface and 5% solid content.

They are designed for magnetic capture, separation and purification of His-tagged proteins in research and process-development workflows.

Product Overview

MNi20UM-10 combines a 20 μm particle size, nickel-affinity surface and convenient magnetic separation.

The beads provide a solid phase for capturing polyhistidine-tagged recombinant proteins from clarified cell lysates, protein solutions and other compatible samples.

The concentrated 5% suspension supports flexible bead dosage, repeated purification experiments, pilot projects and bulk applications.

Technical Specifications

Binding capacity, nickel loading, magnetic response and buffer composition are specification-dependent. Refer to the applicable TDS and COA.

Key Advantages

• Nominal particle size of 20 μm

• Concentrated 5% solids

• Nickel-affinity surface

• Convenient magnetic collection

• Easy washing and buffer exchange

• Suitable for His-tagged protein capture

• Suitable for tube and plate workflows

• Supports manual process development

• Flexible packaging volumes

• OEM and bulk production available

How Do Nickel Magnetic Beads Work?

Nickel-affinity purification is based on immobilized metal affinity chromatography, commonly called IMAC.

Histidine residues in a His tag interact with immobilized nickel ions on the bead surface. A compatible magnet collects the beads while unbound sample components are removed.

After washing, the captured protein can be eluted using an optimized buffer system.

Applications of 20um Nickel Magnetic Beads

His-Tagged Protein Purification

MNi20UM-10 is suitable for capturing and purifying recombinant proteins containing accessible polyhistidine tags.

Recombinant Protein Screening

The beads can support screening of His-tagged protein expression from multiple cell lysate or protein samples.

Magnetic Protein Separation

A compatible magnetic separator enables convenient bead collection, washing, buffer exchange and liquid removal.

Protein Pull-Down Assays

His-tagged bait proteins may be immobilized on the beads for protein–protein interaction and pull-down studies.

His-Tagged Protein Immobilization

MNi20UM-10 can support temporary immobilization of His-tagged proteins for downstream binding and analytical research.

Process Development

The beads may be evaluated for purification-condition optimization, bead dosage studies and scale-up development.

Automated Purification Research

MNi20UM-10 can be evaluated in multiwell plates and compatible automated magnetic-separation systems.

Native and Denaturing Purification

Nickel-affinity purification may be developed under native or selected denaturing conditions after compatibility has been confirmed.

Benefits of 20um Particle Size

The 20 μm particle format offers convenient handling during magnetic collection, washing and resuspension.

It is suitable for:

• Microcentrifuge tube workflows

• Magnetic separation racks

• Multiwell plate testing

• Repeated washing steps

• Protein pull-down experiments

• Pilot purification studies

The larger particle size may also simplify bead recovery and liquid removal in compatible magnetic systems.

Benefits of 5% Solid Content

MNi20UM-10 is supplied at 5% solids as a concentrated magnetic bead suspension.

Users can adjust the bead dosage according to sample volume, target concentration and purification requirements.

The concentrated format supports repeated experiments, customized protocols and larger-volume projects.

Typical His-Tag Purification Workflow

1. Resuspend the Beads

Mix the suspension thoroughly before sampling to obtain a uniform bead concentration.

2. Wash and Equilibrate

Collect the beads with a magnet, remove the storage liquid and equilibrate them with a compatible binding buffer.

3. Add the Protein Sample

Add clarified lysate or protein solution and mix gently to promote interaction between the His tag and nickel-affinity surface.

4. Perform Magnetic Separation

Collect the beads with a compatible magnet and remove the unbound fraction.

5. Wash the Beads

Wash the beads to reduce nonspecific proteins and other sample components.

6. Elute the Target Protein

Elute the captured protein using an optimized imidazole, pH-based or other compatible method.

Conditions should be optimized for each target protein and sample matrix.

Buffer Compatibility

Nickel-affinity performance may be affected by:

• Buffer pH

• Salt concentration

• Imidazole concentration

• Detergents

• Reducing agents

• Denaturants

• Chelating agents

• Sample viscosity

Strong chelating agents may remove nickel ions and reduce binding performance. Compatibility should be verified before use.

Factors Affecting Protein Recovery

Protein recovery and purity depend on:

• His-tag accessibility

• Position of the His tag

• Target protein concentration

• Bead-to-sample ratio

• Incubation time

• Mixing efficiency

• Wash stringency

• Elution conditions

• Sample composition

Small-scale optimization is recommended before pilot or batch processing.

Quality and Batch Documentation

Available documentation may include:

• Product code

• Batch number

• Nominal particle size

• Solid content

• Product appearance

• Surface type

• Certificate of Analysis

• Safety Data Sheet

• Technical Data Sheet

Binding capacity and magnetic response should only be stated according to the applicable SHBC product specification.

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies functional magnetic beads for protein purification, biomolecule separation and assay development.

SHBC supply advantages include:

• Multiple magnetic bead sizes

• Nickel and other functional surfaces

• Laboratory sample supply

• Pilot-scale manufacturing

• Bulk production capability

• Customized solid content

• Flexible packaging volumes

• OEM and private-label services

• Technical product-selection support

Customized Nickel Magnetic Beads

Customization options may include:

• Particle diameter

• Particle-size distribution

• Solid content

• Nickel-affinity surface

• Chelating ligand

• Dispersion medium

• Buffer formulation

• Packaging volume

• OEM labeling

• Bulk production

Storage and Handling

Store MNi20UM-10 according to the product label, TDS, COA and SDS.

Thoroughly resuspend the beads before sampling. Avoid allowing the magnetic beads to dry during washing and purification.

Use clean laboratory tools and a compatible magnetic separator. Do not freeze unless freezing compatibility is confirmed in the product specification.

Frequently Asked Questions

What is MNi20UM-10?

MNi20UM-10 is a 20 μm nickel-affinity magnetic bead suspension with a solid content of 5%.

What are nickel magnetic beads used for?

They are mainly used for magnetic capture, purification, separation and immobilization of His-tagged proteins.

What is IMAC?

IMAC means immobilized metal affinity chromatography. It uses immobilized metal ions to capture proteins containing accessible histidine residues.

Can MNi20UM-10 purify 6×His-tagged proteins?

The beads are designed for polyhistidine-tagged protein capture. Performance depends on tag accessibility, sample composition and purification conditions.

Is centrifugation required?

A compatible magnet can be used to collect the beads, so centrifugation is generally not required for bead separation.

Can imidazole be used?

Imidazole is commonly evaluated during binding, washing and elution. The optimal concentration depends on the target protein and sample.

Can EDTA be used?

Strong chelating agents such as EDTA may remove nickel ions and reduce affinity performance.

Can the beads be used under denaturing conditions?

Selected denaturing conditions may be evaluated after compatibility with the bead formulation has been confirmed.

What is the binding capacity?

Binding capacity depends on the surface chemistry, target protein and purification conditions. Refer to the applicable SHBC technical specification.

Can MNi20UM-10 be used in automated systems?

The beads may be evaluated in compatible multiwell plate and automated magnetic-separation workflows.

Are bulk quantities available?

Yes. SHBC supports laboratory samples, pilot projects, customized packaging and bulk production.

Request MNi20UM-10 Samples and Quotations

Contact Shanghai SanYu Biotechnology Co., Ltd. for MNi20UM-10 samples, technical specifications, customized packaging and bulk quotations.

SHBC supplies nickel magnetic beads for His-tagged protein purification, affinity capture, protein immobilization and research applications.

For Research Use Only.