200nm Carboxyl Red-Fluo Microspheres CFR200-10 Overview

CFR200-10 200nm Carboxyl Red-Fluo Microspheres are fluorescent polymer microspheres developed for lateral flow immunoassay (LFIA), immunochromatography reagent development, antibody conjugation, and diagnostic assay manufacturing.

With a precisely controlled particle size of 200nm, red fluorescent signal, carboxyl-functionalized surface, and 1% solids concentration, CFR200-10 provides a reliable fluorescent labeling solution for biotechnology companies, IVD reagent manufacturers, and research organizations.

The surface carboxyl groups allow covalent coupling with antibodies, antigens, proteins, peptides, and other biomolecules through EDC/NHS activation chemistry.

CFR200-10 is designed for applications ranging from laboratory assay development to pilot validation and bulk production of fluorescent immunochromatography reagents.

Product Specifications of CFR200-10 Red Fluorescent Microspheres

Key Features of 200nm Carboxyl Red-Fluo Microspheres

200nm Particle Size for High-Performance Immunochromatography

CFR200-10 features a 200nm particle diameter designed for fluorescent immunoassay development.

Compared with smaller nanoscale fluorescent particles, 200nm microspheres provide:

• Stronger fluorescence intensity per particle

• Higher dye loading capacity

• Efficient biomolecule coupling

• Stable conjugate formation

• Good membrane migration performance

The 200nm size range provides a balance between optical signal strength and biological interaction, making it suitable for many lateral flow assay development projects.

For researchers developing fluorescent LFIA platforms, particle size selection directly affects:

• Signal intensity

• Conjugate mobility

• Test line capture efficiency

• Background performance

• Assay sensitivity

Stable Red Fluorescence for Quantitative Detection

CFR200-10 contains red fluorescent dye incorporated into the polymer matrix.

The stable fluorescent signal enables applications including:

• Fluorescent lateral flow assays

• Quantitative immunochromatography

• Reader-based detection systems

• Digital diagnostic platforms

• Multiplex assay development

Compared with traditional visual labels, fluorescence-based detection allows objective measurement and digital analysis.

This makes CFR200-10 suitable for companies developing quantitative rapid diagnostic technologies.

Carboxyl Surface Chemistry for Antibody and Protein Coupling

The surface of CFR200-10 contains carboxyl functional groups (-COOH).

Through EDC/NHS activation chemistry, the microspheres can be covalently coupled with:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Nucleic acid probes

Compared with passive adsorption methods, covalent coupling provides stronger attachment and improved conjugate stability.

Uniform Particle Size and Reliable Batch Consistency

Consistent microsphere performance is essential for commercial reagent development.

CFR200-10 provides:

• Controlled particle size distribution

• Stable aqueous suspension

• Reproducible fluorescence performance

• Consistent coupling behavior

• Reliable batch-to-batch quality

These characteristics support the transition from laboratory research to industrial production.

Applications of CFR200-10 in Fluorescent Lateral Flow Assays

Fluorescent Immunochromatography Development

CFR200-10 is designed for fluorescent lateral flow assay development.

Potential applications include:

• Infectious disease detection research

• Biomarker testing

• Food safety analysis

• Veterinary diagnostics

• Environmental monitoring

• Pharmaceutical quality research

The red fluorescent signal can be measured using compatible fluorescence readers.

Antibody Conjugation and Biomolecule Labeling

Carboxyl fluorescent microspheres provide an effective platform for biological molecule immobilization.

Common conjugation targets include:

• Detection antibodies

• Capture antibodies

• Antigens

• Proteins

• Peptides

• DNA/RNA probes

Researchers can optimize coupling conditions according to assay requirements.

Quantitative Immunoassay Platforms

Fluorescent microsphere-based assays support:

• Quantitative signal measurement

• Automated reader analysis

• Reduced visual interpretation errors

• Improved assay evaluation

CFR200-10 can be integrated into next-generation immunoassay platforms.

Diagnostic Reagent Research and Manufacturing

CFR200-10 supports the complete development workflow:

• Raw material evaluation

• Fluorescent conjugate preparation

• Strip development

• Performance validation

• Pilot production

• Bulk manufacturing

How Do 200nm Fluorescent Microspheres Work in LFIA?

A typical fluorescent lateral flow assay process includes:

Step 1: Fluorescent Conjugate Preparation

CFR200-10 microspheres are coupled with detection antibodies using carboxyl chemistry.

Step 2: Sample Application

The biological sample is added to the test strip.

Step 3: Target Binding

Target molecules bind with fluorescent antibody conjugates.

Step 4: Membrane Migration

The complex migrates through the nitrocellulose membrane.

Step 5: Test Line Capture

The target-conjugate complex is captured at the detection line.

Step 6: Fluorescence Reading

The fluorescent signal is measured by an optical reader.

The final assay performance depends on:

• Particle characteristics

• Antibody affinity

• Coupling efficiency

• Membrane selection

• Buffer formulation

• Reader sensitivity

Antibody Coupling Process of CFR200-10 Carboxyl Microspheres

Carboxyl Activation

The surface carboxyl groups are activated using EDC/NHS chemistry.

Typical optimization factors include:

• Activation pH

• Reaction time

• Activator concentration

• Particle concentration

Antibody Conjugation

Activated microspheres react with amino groups on antibodies or proteins.

Optimization parameters:

• Antibody concentration

• Particle-to-protein ratio

• Reaction temperature

• Incubation time

Blocking and Purification

After conjugation:

• Remaining active sites are blocked

• Free proteins are removed

• Fluorescent conjugates are stabilized

Conjugate Evaluation

Recommended evaluation includes:

• Particle size distribution

• Fluorescence intensity

• Protein coupling efficiency

• Dispersion stability

• LFIA performance testing

Advantages of Fluorescent Microspheres Compared with Colloidal Gold

Quality Control and Bulk Production Capability

For IVD companies, consistent raw materials are critical for product reliability.

CFR200-10 quality control includes:

Particle Size Analysis

Ensures consistent migration and conjugation.

Fluorescence Performance Testing

Evaluates optical signal stability.

Surface Functional Group Testing

Confirms available carboxyl groups.

Solid Content Control

Maintains formulation consistency.

Batch Consistency Evaluation

Supports reliable commercial production.

Customization Options for CFR200-10

SHBC provides customized fluorescent microsphere solutions.

Particle Size Customization

Available sizes can be optimized according to:

• Membrane structure

• Detection requirements

• Assay design

Fluorescence Customization

Options include:

• Different fluorescent colors

• Customized emission characteristics

• Reader compatibility

Surface Chemistry Customization

Available functional groups:

• Carboxyl

• Amino

• Streptavidin

• Other customized chemistry

Solid Content Customization

Concentration can be adjusted according to:

• Coupling workflow

• Shipping requirements

• Manufacturing process

OEM and Bulk Manufacturing Support

SHBC provides:

• Research samples

• Pilot batches

• Bulk production

• Customized packaging

• Long-term supply cooperation

Frequently Asked Questions About CFR200-10

What are 200nm Carboxyl Red-Fluo Microspheres?

CFR200-10 are 200nm fluorescent polymer microspheres with carboxyl functional surfaces designed for LFIA, immunoassay research, and antibody conjugation.

Can CFR200-10 be used for immunochromatography?

Yes. CFR200-10 is suitable for fluorescent lateral flow assay development and diagnostic reagent research.

How are antibodies coupled to CFR200-10?

Antibodies are commonly attached through EDC/NHS chemistry to form stable covalent bonds.

Why choose 200nm fluorescent microspheres?

200nm microspheres provide strong fluorescence intensity, stable conjugation, and reliable assay performance.

Can CFR200-10 be supplied in bulk?

Yes. SHBC provides research quantities, pilot production, and bulk manufacturing supply.

Can the product specifications be customized?

Yes. Particle size, fluorescence properties, functional groups, solid content, and packaging can be customized.

Why Choose SHBC CFR200-10 Carboxyl Red-Fluo Microspheres?

SHBC specializes in fluorescent microsphere materials for immunodiagnostic research and reagent manufacturing.

CFR200-10 provides:

• 200nm controlled particle size

• Red fluorescent labeling

• Carboxyl functional surface

• Stable antibody conjugation

• LFIA development support

• Bulk manufacturing capability

• OEM customization services

Contact SHBC for technical specifications, samples, and bulk quotation for 200nm Carboxyl Red-Fluo Microspheres CFR200-10.