300nm Carboxyl Blue-Fluo Microspheres CFB300-10

300nm Carboxyl Blue-Fluo Microspheres CFB300-10

SHBC CFB300-10 are 300nm blue fluorescent microspheres with reactive carboxyl groups on the particle surface.

With excitation at 366nm and emission at 460nm, these microspheres provide a clear blue fluorescent signal for fluorescence immunoassays, lateral flow assay development, biosensors, particle tracing and biomolecule conjugation.

Product Specifications

Key Product Advantages

• Blue fluorescence at 460nm

• Compatible with 366nm UV excitation

• Reactive carboxyl-functionalized surface

• Uniform 300nm particle size

• Good aqueous dispersion

• Suitable for antibody and protein coupling

• Compatible with multiple fluorescence applications

• Available for research and batch production

Why Choose 300nm Blue Fluorescent Microspheres?

The 300nm particle size provides a practical combination of fluorescence signal, biomolecule-loading surface and suspension performance.

CFG300-10 is suitable for projects requiring:

• UV-excited blue fluorescent labels

• Stable particle-based signals

• Covalent biomolecule immobilization

• Fluorescent membrane assay development

• Biosensor signal enhancement

• Multicolor assay differentiation

Carboxyl Surface for Biomolecule Coupling

CFB300-10 contains surface carboxyl groups that can be activated for covalent conjugation with primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amine-containing molecules

EDC/NHS chemistry can be used to form stable amide bonds between the microspheres and target biomolecules.

Main Applications

Fluorescent Lateral Flow Assays

CFB300-10 can be evaluated as a fluorescent reporter particle for qualitative and quantitative lateral flow assay development.

Potential advantages include:

• Clear blue fluorescent signal

• Good particle visibility

• Antibody conjugation capability

• Compatibility with suitable UV fluorescence readers

• Potential for multicolor test-line differentiation

Membrane, running buffer and reader compatibility should be optimized for each assay.

Fluorescence Immunoassays

The microspheres can carry both fluorescent signals and biological recognition molecules.

Suitable research formats include:

• Sandwich immunoassays

• Competitive immunoassays

• Antibody detection

• Antigen detection

• Particle-based binding assays

Antibody and Protein Conjugation

The carboxyl surface supports covalent immobilization of antibodies, proteins and peptides for fluorescent probe preparation.

Biosensors and Biochips

CFB300-10 can serve as a fluorescent signal carrier in:

• Biosensors

• Microarrays

• Biochips

• Microfluidic devices

• Surface-based detection systems

Fluorescence Imaging and Particle Tracing

The 460nm blue emission supports particle localization, material distribution analysis and fluorescence microscopy research using compatible optical filters.

Multiplex Detection

Blue fluorescent microspheres can be combined with green, red or other fluorescent particle populations for multicolor and multiplex assay development.

Diagnostic Reagent Development

CFB300-10 is suitable for laboratory evaluation, assay optimization, pilot production and fluorescence-based diagnostic reagent research.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash with a suitable activation buffer.

3. Activate the carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling efficiency and fluorescence performance.

Reaction pH, reagent concentration, coupling time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix thoroughly before sampling.

• Protect the suspension from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid repeated freezing and thawing.

• Use clean, low-binding laboratory consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage requirements.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density adjustment

• Alternative functional groups

• Solids-content adjustment

• Custom suspension buffers

• Custom packaging

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, biosensing and diagnostic reagent development.

SHBC provides:

• Controlled particle-size production

• Stable fluorescence performance

• Functional surface modification

• Reliable batch-to-batch consistency

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale and bulk production

Frequently Asked Questions

What is CFB300-10?

CFB300-10 is a 300nm carboxyl-functionalized blue fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelength is 366nm, and the emission wavelength is 460nm.

Can CFB300-10 be coupled with antibodies?

Yes. Its surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFB300-10 suitable for lateral flow assays?

It can be evaluated as a fluorescent lateral flow reporter. Membrane pore size, buffer composition and optical reader settings should be optimized.

What equipment can detect the microspheres?

Use a fluorescence reader, microscope or optical system with compatible UV excitation and blue emission filters.

Can it be used for multiplex detection?

Yes. Blue fluorescent particles can be combined with other fluorescence colors or particle populations for multiplex assay research.

Can SHBC provide samples?

Yes. Samples are available for coupling evaluation, fluorescence testing and assay development.

Is bulk production available?

Yes. SHBC supports pilot-scale orders and bulk manufacturing for biotechnology and diagnostic reagent companies.

Can the product be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, buffer and packaging can be customized.

Ordering Information

Product Name: 300nm Carboxyl Blue-Fluo Microspheres
Catalog Number: CFB300-10
Particle Size: 300nm
Solids Content: 1%
Excitation Wavelength: 366nm
Emission Wavelength: 460nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.