30nm Carboxyl Green-Fluo Microspheres CFG030-10 0.5%

30nm Carboxyl Green-Fluo Microspheres CFG030-10

SHBC CFG030-10 are 30 nm carboxyl-functionalized green fluorescent microspheres developed for fluorescent immunoassays, lateral flow tests, biosensors and biomolecule labeling.

The particles feature excitation at 470/488 nm, emission at 525 nm and 0.5% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size distribution, fluorescence intensity, carboxyl content and dispersion information are available in the Certificate of Analysis.

Green Fluorescence at 470/488–525nm

CFG030-10 can be excited using approximately 470 nm or 488 nm light and produces green fluorescence centered near 525 nm.

The optical profile is compatible with suitable fluorescence readers, imaging systems and instruments equipped with green fluorescence filter sets.

Key Advantages

Small 30nm Particle Size

The nanoscale particle size supports rapid diffusion, stable colloidal dispersion and efficient migration through compatible porous materials.

Green Fluorescent Signal

The 525 nm emission provides a clear green signal for fluorescence-based qualitative and quantitative detection.

High Specific Surface Area

The small particle diameter provides a large total surface area for biomolecule immobilization.

Reactive Carboxyl Surface

Surface COOH groups support covalent coupling of antibodies, antigens, proteins, peptides and other amino-containing molecules.

0.5% Aqueous Suspension

The suspension is convenient for assay development, conjugation optimization and production scale-up.

Custom and Bulk Supply

SHBC supports samples, customized specifications, pilot production and industrial batch supply.

Main Applications

Fluorescent Lateral Flow Assays

CFG030-10 can be evaluated as a fluorescent reporter nanoparticle in qualitative and quantitative lateral flow tests.

Fluorescence Immunoassays

Suitable for sandwich, competitive and indirect fluorescence immunoassay formats.

Antibody and Antigen Conjugation

Surface carboxyl groups allow covalent immobilization of antibodies, antigens and recombinant proteins.

Biosensor Development

Can be used as a fluorescent signal carrier in optical biosensors and surface-based detection systems.

Protein and Biomolecule Labeling

Suitable for coupling proteins, peptides, enzymes and other amino-containing biomolecules.

Nanoparticle Tracing

Can be evaluated in membrane transport, filtration, fluid-flow and nanoparticle-distribution research.

Diagnostic Reagent Development

Suitable for research and production of fluorescent detection reagents for medical, veterinary and food-safety testing.

Benefits for Fluorescent Immunoassays

CFG030-10 provides:

• Green fluorescence at 525 nm

• Reactive COOH coupling sites

• High total coupling surface

• Good colloidal mobility

• Compatibility with quantitative readers

• Flexible biomolecule modification

• Sample and bulk supply

• Custom fluorescence options

Actual assay sensitivity depends on particle loading, fluorescence intensity, conjugation efficiency and reader performance.

Biomolecule Coupling

Surface carboxyl groups can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.

A general coupling workflow includes:

1. Disperse and wash the particles.

2. Activate the carboxyl groups with EDC and NHS.

3. Add the antibody or target protein.

4. Block remaining reactive sites.

5. Purify and resuspend the conjugate.

6. Evaluate coupling and fluorescence performance.

Activation pH, reagent ratio, protein concentration and reaction time should be optimized for each biomolecule.

LFIA Development Considerations

For lateral flow applications, evaluate:

• Antibody-to-particle ratio

• Particle concentration

• Fluorescence intensity

• Nitrocellulose membrane type

• Conjugate pad release

• Running buffer composition

• Surfactant concentration

• Test-line capture density

• Background fluorescence

• Reader filter settings

The small particles may migrate rapidly, but sufficient fluorescent particle accumulation is required for a strong test-line signal.

Dispersion and Handling

Before use:

• Mix by gentle inversion

• Use controlled bath sonication when required

• Avoid excessive bubbles and foam

• Prevent the particles from drying

• Protect the suspension from strong light

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

• Use clean laboratory equipment

Because 30 nm particles may be difficult to pellet using standard centrifugation, purification methods should be selected according to the conjugation process.

Purification Options

Suitable purification methods may include:

• Ultracentrifugation

• Size-exclusion chromatography

• Dialysis

• Tangential flow filtration

• Ultrafiltration

The selected method should minimize particle loss, aggregation and fluorescence reduction.

Application Limitations

Individual 30 nm particles are generally too small for direct detection by conventional flow cytometry.

Their fluorescence signal should be evaluated as a particle population, accumulated assay signal or conjugated nanoparticle complex.

High salt concentrations, unsuitable solvents or incorrect pH may cause aggregation or reduce fluorescence performance.

Custom Carboxyl Green Fluorescent Microspheres

SHBC can customize fluorescent microspheres according to assay requirements.

Customization options may include:

• Custom particle size

• Custom excitation and emission wavelength

• Different fluorescence colors

• Carboxyl group density

• Fluorescence intensity

• Solid content

• Dispersion buffer

• Surface modification

• Custom packaging

• OEM and ODM production

Please provide the target application, biomolecule, required wavelength and order quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Dispersion stability data

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional particle materials.

SHBC provides:

• Controlled nanoparticle manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFG030-10?

CFG030-10 is a 30 nm carboxyl-functionalized green fluorescent microsphere suspension with 0.5% solid content.

What are the excitation and emission wavelengths?

The excitation wavelengths are 470/488 nm, and the emission wavelength is 525 nm.

What is CFG030-10 mainly used for?

It can be evaluated for fluorescent immunoassays, lateral flow tests, biosensors, protein labeling and nanoparticle tracing.

Can antibodies be coupled to CFG030-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling method is recommended?

EDC/NHS chemistry is commonly used to activate surface carboxyl groups for protein immobilization.

Is CFG030-10 suitable for lateral flow assays?

Yes. Membrane type, particle loading, conjugate release and fluorescence reader settings should be optimized.

Can CFG030-10 be excited with a 488nm laser?

Yes. The specified excitation range includes 488 nm, with green emission near 525 nm.

Can the particles be separated by standard centrifugation?

Standard low-speed centrifugation is generally unsuitable for 30 nm particles. Ultracentrifugation, filtration, dialysis or chromatography may be required.

How should CFG030-10 be dispersed?

Use gentle mixing or controlled bath sonication while avoiding excessive foam, high salt concentration and particle drying.

Can CFG030-10 be customized?

Yes. Particle size, fluorescence wavelength, fluorescence intensity, carboxyl density, solid content, buffer and packaging can be customized.

Are samples and bulk quantities available?

Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.

Request a Sample or Quotation

Contact SHBC for CFG030-10 samples, technical specifications, conjugation support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Assay format

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 30nm Carboxyl Green-Fluo Microspheres
Catalog Number: CFG030-10
Particle Size: 30 nm
Solid Content: 0.5%
Excitation/Emission: 470/488–525 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.