320 um Dextran Gel Filtration Media G-25 C TopGF G-25 C

320 μm Dextran Gel Filtration Media G-25 C

SHBC TopGF G-25 C is a dextran-based gel filtration medium with a nominal particle size of 320 μm.

It is designed for rapid group separation, desalting, buffer exchange and removal of low-molecular-weight substances from antibodies, proteins, enzymes and other biomolecules.

The coarse particle format is suitable for research laboratories, immunoassay developers and manufacturers requiring scalable purification media.

TopGF G-25 C Technical Specifications

Size-Exclusion Separation Principle

TopGF G-25 C separates molecules according to their effective molecular size.

Large biomolecules are excluded from most pores and pass through the column first. Salts and other small molecules enter the internal pores and elute later.

The medium does not require target biomolecules to bind to the matrix, making it suitable for rapid group separation and buffer replacement.

Key Advantages of TopGF G-25 C

• Dextran-based gel filtration matrix

• Nominal 320 μm coarse particles

• Rapid desalting and buffer exchange

• Separation without ligand binding

• Suitable for antibodies and proteins

• Helps remove salts and small molecules

• Supports gravity and low-pressure workflows

• Coarse particles allow efficient liquid flow

• Suitable for laboratory and production scale-up

• Available for bulk manufacturing supply

Why Choose a 320 μm Coarse Grade?

The coarse particle format is designed for rapid group separation rather than high-resolution analytical fractionation.

Potential advantages include:

• Faster buffer flow

• Lower flow resistance

• Convenient column packing

• Suitable processing of larger sample volumes

• Reduced processing time

• Easier scale-up to wider columns

Fine-grade media may provide higher resolution, while coarse media are generally preferred for rapid desalting and industrial buffer exchange.

Immunoassay Manufacturing Applications

Antibody Desalting

TopGF G-25 C can be used to remove salts and small buffer components from antibody preparations.

Typical processes include:

• Desalting after antibody purification

• Removal of ammonium sulfate

• Exchange into assay-compatible buffers

• Preparation before antibody conjugation

• Preparation before magnetic-bead coupling

Antibody Buffer Exchange

Antibodies can be transferred into a selected formulation or coupling buffer before:

• Epoxy magnetic-bead coupling

• Carboxyl magnetic-bead coupling

• Fluorescent labeling

• Enzyme conjugation

• Biotinylation

• Immunoassay formulation

Removal of Free Labeling Reagents

TopGF G-25 C may be evaluated for separating proteins from compatible low-molecular-weight reagents, including:

• Free fluorescent dyes

• Unreacted biotin reagents

• Small coupling reagents

• Buffer salts

• Reducing agents

• Reaction by-products

The suitability of the medium should be confirmed according to the molecular size of the target and impurity.

Protein and Enzyme Applications

TopGF G-25 C can also be evaluated for:

• Protein desalting

• Enzyme buffer exchange

• Antigen purification

• Protein formulation

• Removal of low-molecular-weight contaminants

• Sample preparation before chromatography

• Biomolecule cleanup after labeling

• Purification-process intermediate treatment

G-25-type dextran media are widely positioned for industrial desalting and buffer exchange with good biomolecule recovery and limited sample dilution.

Suitable Samples

TopGF G-25 C can be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Diagnostic antigens

• Enzymes

• Protein conjugates

• Peptides above the exclusion range

• Other water-soluble biomolecules

Application performance depends on molecular size, sample concentration, viscosity and column conditions.

General Operating Workflow

1. Hydrate the Medium

Hydrate the dry gel filtration medium completely using purified water or the selected buffer.

Allow sufficient time for uniform swelling before column packing.

2. Prepare the Slurry

Mix the hydrated medium gently to form a uniform slurry.

Avoid introducing excessive air bubbles.

3. Pack the Column

Transfer the slurry into a suitable chromatography column and allow the bed to settle evenly.

Avoid cracks, channels and trapped air.

4. Equilibrate the Column

Equilibrate the packed bed with the target buffer until the outlet conditions are stable.

5. Load the Sample

Apply the sample evenly onto the top of the packed bed.

Avoid disturbing the bed surface.

6. Elute the Biomolecule

Elute using the target buffer. Larger biomolecules normally elute before salts and other small molecules.

7. Collect and Evaluate

Collect the biomolecule-containing fraction and evaluate:

• Protein concentration

• Conductivity

• Recovery

• Purity

• Buffer composition

• Assay activity

Factors Affecting Separation Performance

Important operating factors include:

• Column dimensions

• Packed-bed quality

• Sample volume

• Sample concentration

• Molecular-size difference

• Flow rate

• Buffer composition

• Sample viscosity

• Temperature

• Fraction collection volume

For group separation, sample loading and column volume should be optimized to achieve efficient recovery and contaminant removal.

Column Packing Recommendations

For consistent performance:

• Hydrate the resin completely.

• Prepare a uniform slurry.

• Remove trapped air before packing.

• Avoid excessive compression.

• Maintain a level bed surface.

• Prevent the packed bed from drying.

• Confirm stable flow before sample loading.

• Evaluate bed integrity after repeated use.

Process Scale-Up

When scaling from laboratory testing to production, maintain consistent:

• Bed height

• Sample-volume-to-bed-volume ratio

• Linear flow velocity

• Buffer composition

• Sample concentration

• Fraction collection strategy

• Cleaning procedure

• Performance acceptance criteria

Coarse G-25 media are commonly selected for high-throughput group separation and industrial buffer-exchange processes.

TopGF G-25 C vs. Fine-Grade Gel Filtration Media

TopGF G-25 C is recommended when rapid desalting and buffer exchange are more important than analytical resolution.

TopGF G-25 C vs. Dialysis

The preferred method depends on sample volume, processing time and recovery requirements.

Quality Control for Bulk Production

Recommended evaluation items include:

• Particle-size consistency

• Swelling consistency

• Packed-bed appearance

• Flow performance

• Desalting efficiency

• Biomolecule recovery

• Conductivity reduction

• Lot-to-lot consistency

• Storage stability

• Microbial control

Why Choose SHBC TopGF G-25 C?

• Nominal 320 μm coarse particles

• Cross-linked dextran matrix

• Designed for desalting and buffer exchange

• Suitable for antibody and protein processing

• Supports immunoassay reagent development

• Laboratory samples available

• Stable batch production

• Bulk packaging options

• Scalable manufacturing supply

• Technical support for product selection

Storage and Handling

Store TopGF G-25 C according to the product label and technical documentation.

Recommended practices:

• Keep unused dry media tightly sealed.

• Protect the product from moisture and contamination.

• Use clean water, buffers and containers.

• Avoid strong mechanical damage to hydrated particles.

• Do not allow a packed column to dry.

• Establish a validated storage solution for hydrated media.

• Review the SDS before laboratory or industrial use.

Frequently Asked Questions

What is TopGF G-25 C?

TopGF G-25 C is a coarse dextran-based gel filtration medium used for desalting, buffer exchange and group separation.

What is the particle size?

The nominal particle size is 320 μm.

What is the main application?

Its main applications are protein desalting, antibody buffer exchange and removal of low-molecular-weight substances.

Can it be used for antibody purification?

It can be used for antibody desalting and buffer exchange. It is not designed to replace affinity chromatography for selective antibody capture.

Can it remove salts from proteins?

Yes. Large proteins elute before salts and other small molecules under suitable column conditions.

Can it be used before magnetic-bead coupling?

Yes. It can be evaluated for transferring antibodies or proteins into a compatible coupling buffer.

Can it remove free fluorescent dyes?

It may be used when the free dye is sufficiently smaller than the labeled protein. The separation should be validated for each labeling reagent.

Is it suitable for high-resolution protein fractionation?

TopGF G-25 C is primarily designed for group separation, desalting and buffer exchange rather than high-resolution analytical separation.

Is column optimization necessary?

Yes. Sample volume, column size, flow rate, buffer and fraction collection should be optimized for each process.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk supply for research laboratories, immunoassay developers and biotechnology manufacturers.

Request TopGF G-25 C Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation and bulk quotations.

Please provide:

• Target biomolecule

• Current buffer

• Required new buffer

• Sample volume

• Protein concentration

• Column dimensions

• Required processing capacity

• Estimated annual demand

TopGF G-25 C is supplied for research and process development. Customers should validate the medium for their final manufacturing or diagnostic application.