Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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CFR3UM-10
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SHBC
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3µm
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10ml,20ml,50ml ,500ml,1000ml
3um Carboxyl Red-Fluo Microspheres CFR3UM-10
SHBC CFR3UM-10 are 3 μm carboxyl-functionalized red fluorescent microspheres developed for flow cytometry, fluorescence imaging, cell labeling, particle tracing and biomolecule immobilization.
The microspheres feature excitation at 535 nm, emission at 610 nm and 1% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and other amino-containing molecules.
Product Specifications
Parameter | Specification |
|---|---|
Product Name | Carboxyl Red-Fluo Microspheres |
Catalog Number | CFR3UM-10 |
Nominal Particle Size | 3 μm |
Solid Content | 1% |
Surface Group | Carboxyl, COOH |
Excitation Wavelength | 535 nm |
Emission Wavelength | 610 nm |
Fluorescence Color | Red |
Supply Form | Aqueous Suspension |
Brand | SHBC |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Batch-specific particle size, size distribution, carboxyl content and fluorescence data are available in the Certificate of Analysis.
Red Fluorescence at 535/610nm
CFR3UM-10 can be excited at approximately 535 nm and produces red fluorescence centered at approximately 610 nm.
The optical profile is suitable for compatible fluorescence microscopes, flow cytometers, imaging systems and fluorescence readers.
Key Advantages
Bright Red Fluorescence
The 535/610 nm fluorescence profile provides a clear signal for particle detection, imaging and quantitative analysis.
Uniform 3um Particle Size
Controlled particle sizing supports repeatable fluorescence, coupling and analytical performance.
High Signal per Particle
The 3 μm particle size provides a strong fluorescent signal and a large surface area for biomolecule immobilization.
Reactive Carboxyl Surface
Surface COOH groups support covalent attachment of antibodies, antigens, proteins, peptides and other amino-containing molecules.
Easy Particle Detection
The micrometer-scale particles are suitable for fluorescence microscopy, flow cytometry and particle-tracking studies.
Custom and Bulk Supply
SHBC supports samples, customized specifications, pilot production and industrial batch supply.
Main Applications
Flow Cytometry
CFR3UM-10 can be used in particle detection, fluorescence channel evaluation and flow-based assay development.
Fluorescence Microscopy
The bright red signal supports particle localization, imaging and distribution studies.
Cell Labeling and Tracking
Suitable for selected cell-binding, cell-surface labeling and particle-tracking experiments.
Phagocytosis and Cellular Uptake Studies
The 3 μm particles can be evaluated in macrophage uptake, phagocytosis and particle–cell interaction research.
Immunoassay Carrier Particles
Surface carboxyl groups allow antibodies or antigens to be immobilized for particle-based immunoassays.
Biomolecule Immobilization
Suitable for covalent coupling of proteins, enzymes, peptides and other amino-containing biomolecules.
Particle Transport Studies
Can be used in microfluidics, filtration, fluid-flow and particle-transport research.
Instrument and Method Development
Suitable for developing fluorescence detection, imaging and particle-analysis methods.
Biomolecule Coupling
The carboxyl surface can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.
A typical coupling workflow includes:
Wash and redisperse the microspheres.
Activate surface carboxyl groups with EDC and NHS.
Add the antibody or target protein.
Block remaining active sites.
Wash and resuspend the conjugated particles.
Evaluate coupling efficiency and fluorescence performance.
Activation pH, reagent concentration, protein loading and reaction time should be optimized for each biomolecule.
Coupling Advantages
CFR3UM-10 provides:
Active surface carboxyl groups
Large coupling surface per particle
Stable covalent immobilization
Easy separation by centrifugation
Strong fluorescence detection
Suitable particle size for microscopy
Compatibility with particle-based assays
Support for customized conjugation
Flow Cytometry Considerations
Before flow cytometry analysis, optimize:
Sample concentration
Flow rate
Fluorescence detector settings
Excitation compatibility
Emission filter selection
FSC and SSC settings
Particle aggregation control
Gating strategy
A fluorescence channel compatible with 610 nm emission should be selected.
Cell and Imaging Studies
For cellular experiments, evaluate:
Particle concentration
Incubation time
Cell type
Surface ligand density
Blocking conditions
Washing efficiency
Cellular uptake
Background fluorescence
Appropriate negative controls should be included in each experiment.
Dispersion and Handling Guide
Before use:
Mix by gentle inversion or controlled vortexing
Redisperse settled particles completely
Avoid excessive bubbles and foam
Protect the product from strong light
Prevent the microspheres from drying
Avoid repeated freeze-thaw cycles
Confirm buffer compatibility
Avoid prolonged high-speed mixing
Controlled bath sonication may be used when necessary to break weak particle aggregates.
Storage Recommendations
Keep the container tightly closed and protected from light.
Use clean laboratory tools to avoid contamination. Do not return unused material to the original container.
Refer to the product label, Technical Data Sheet and Certificate of Analysis for batch-specific storage conditions.
Custom Carboxyl Fluorescent Microspheres
SHBC can customize fluorescent microspheres according to application requirements.
Customization options may include:
Custom particle size
Custom excitation and emission wavelengths
Different fluorescence colors
Carboxyl group density
Solid content
Dispersion buffer
Surface modification
Biomolecule conjugation
Custom packaging
OEM and ODM production
Please provide the application, target wavelength, particle size and required quantity when requesting customization.
Quality Control and Documents
Available product documents may include:
Certificate of Analysis
Technical Data Sheet
Safety Data Sheet
Particle size report
Particle size distribution data
Fluorescence spectrum
Carboxyl content data
Microscopy images
Batch inspection report
Why Choose SHBC?
Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.
SHBC provides:
Controlled microsphere manufacturing
Stable particle size distribution
Multiple fluorescence wavelengths
Functional surface modification
Batch-to-batch consistency
Sample evaluation support
Pilot and bulk production
OEM and ODM services
Frequently Asked Questions
What is CFR3UM-10?
CFR3UM-10 is a 3 μm carboxyl-functionalized red fluorescent microsphere suspension with 1% solid content.
What are the excitation and emission wavelengths?
The excitation wavelength is 535 nm and the emission wavelength is 610 nm.
What is CFR3UM-10 mainly used for?
It is suitable for flow cytometry, fluorescence microscopy, cell labeling, phagocytosis studies, particle tracing and biomolecule coupling.
Can antibodies be coupled to CFR3UM-10?
Yes. Antibodies and other proteins containing primary amine groups can be covalently coupled to the carboxyl surface.
Which coupling method is recommended?
EDC/NHS chemistry is commonly used to activate carboxyl groups for protein immobilization.
Is CFR3UM-10 suitable for flow cytometry?
Yes. Instrument voltage, scatter settings, fluorescence filters, particle concentration and gating conditions should be optimized.
Can CFR3UM-10 be used for cellular uptake studies?
Yes. The 3 μm particles can be evaluated in phagocytosis, macrophage uptake and particle–cell interaction studies.
Is CFR3UM-10 suitable for conventional lateral flow assays?
The 3 μm size is relatively large for conventional membrane migration. Application testing is required, and smaller particles are generally more suitable for standard lateral flow formats.
How should CFR3UM-10 be dispersed?
Use gentle inversion, controlled vortexing or compatible bath sonication while avoiding excessive foam and particle damage.
Can CFR3UM-10 be customized?
Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer and packaging can be customized.
Are samples and bulk quantities available?
Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.
Request a Sample or Quotation
Contact SHBC for CFR3UM-10 samples, technical specifications, coupling support and bulk pricing.
Please provide:
Target application
Target biomolecule
Detection instrument
Required quantity
Preferred packaging
Estimated annual demand
Product: 3um Carboxyl Red-Fluo Microspheres
Catalog Number: CFR3UM-10
Particle Size: 3 μm
Solid Content: 1%
Excitation/Emission: 535/610 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.