400nm Carboxyl Blue-Fluo Microspheres CFB400-10 1%

400nm Carboxyl Blue-Fluo Microspheres CFB400-10

SHBC CFB400-10 are 400nm blue fluorescent microspheres with reactive carboxyl groups on the particle surface.

With excitation at 366nm and emission at 460nm, these microspheres are designed for fluorescence immunoassays, lateral flow test development, biosensors, biomolecule conjugation and particle-tracing research.

Product Specifications

Key Product Advantages

• Blue fluorescence at 460nm

• Compatible with 366nm UV excitation

• Reactive carboxyl-functionalized surface

• Uniform 400nm nominal particle size

• Strong particle-level fluorescent signal

• Suitable for antibody and protein conjugation

• Compatible with multiple optical assay formats

• Available for research and batch production

Why Choose 400nm Blue Fluorescent Microspheres?

The 400nm particle size provides a useful balance between fluorescence signal, biomolecule-loading surface and particle handling.

CFB400-10 is suitable for projects requiring:

• UV-excited blue fluorescent labels

• Clear optical signal detection

• Stable biomolecule immobilization

• Particle-based fluorescence assays

• Membrane assay development

• Multicolor assay differentiation

The final migration, sensitivity and background should be optimized for each assay system.

Carboxyl Surface for Biomolecule Coupling

CFB400-10 contains reactive carboxyl groups for the covalent immobilization of primary amine-containing biomolecules.

Compatible ligands include:

• Antibodies

• Antigens

• Proteins

• Peptides

• Enzymes

• Oligonucleotides

• Other amine-containing molecules

The surface carboxyl groups can be activated using EDC/NHS chemistry to form stable amide bonds with target biomolecules.

Main Applications

Fluorescent Lateral Flow Assays

CFB400-10 can be evaluated as a blue fluorescent reporter for qualitative and quantitative lateral flow assay development.

Potential benefits include:

• Clear blue fluorescent signal

• Large surface for antibody loading

• Compatibility with suitable UV fluorescence readers

• Potential use in multicolor test systems

• Easy differentiation from green and red labels

Membrane pore size, running buffer, conjugate release and reader settings should be optimized.

Fluorescence Immunoassays

The microspheres can carry both fluorescent signals and biological recognition molecules.

Potential assay formats include:

• Sandwich immunoassays

• Competitive immunoassays

• Antibody detection

• Antigen detection

• Particle-based binding assays

• Fluorescent agglutination research

Antibody and Protein Conjugation

The carboxyl surface supports covalent immobilization of antibodies, proteins, peptides and enzymes for fluorescent probe preparation.

Biosensors and Biochips

CFB400-10 can serve as a fluorescent signal carrier in:

• Biosensors

• Microarrays

• Biochips

• Microfluidic devices

• Surface-based detection systems

• Fluorescence signal amplification platforms

Fluorescence Imaging

The 460nm blue emission supports particle localization and fluorescence imaging using compatible excitation sources and optical filters.

Particle Tracking and Tracing

CFB400-10 can be evaluated for:

• Particle transport research

• Material distribution analysis

• Filtration studies

• Microfluidic tracking

• Surface adsorption research

• Fluid-dispersion studies

Multiplex Detection

Blue fluorescent microspheres can be combined with green, red or other fluorescent particles to create distinguishable labels for multicolor and multiplex assay research.

Recommended Coupling Workflow

1. Mix the microsphere suspension thoroughly.

2. Wash the particles with a suitable activation buffer.

3. Activate the surface carboxyl groups using EDC and NHS.

4. Add the antibody, protein or target ligand.

5. Incubate under optimized reaction conditions.

6. Block the remaining active sites.

7. Wash the conjugated microspheres.

8. Resuspend in a suitable storage buffer.

9. Evaluate coupling efficiency and fluorescent performance.

Coupling pH, reagent concentration, reaction time and blocking conditions should be optimized for each biomolecule.

Handling Recommendations

• Mix thoroughly before sampling.

• Protect the suspension from strong light.

• Avoid prolonged high-power ultrasonication.

• Do not allow the microspheres to dry.

• Avoid freezing and repeated freeze–thaw cycles.

• Use clean, low-binding consumables.

• Confirm buffer compatibility before scale-up.

• Follow the product label for storage conditions.

Customization and Bulk Supply

SHBC supports fluorescent microsphere projects from laboratory evaluation to batch manufacturing.

Customization options include:

• Different particle sizes

• Different fluorescence wavelengths

• Fluorescence intensity adjustment

• Surface carboxyl density adjustment

• Alternative functional groups

• Solids-content adjustment

• Custom suspension buffers

• Custom packaging

• OEM and ODM production

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. manufactures functional microspheres and magnetic beads for fluorescence detection, immunoassays, biosensing and diagnostic reagent development.

SHBC provides:

• Controlled particle-size production

• Functional surface modification

• Batch-to-batch quality control

• Biomolecule coupling support

• Research samples

• Flexible packaging

• Pilot-scale supply

• Bulk manufacturing

Frequently Asked Questions

What is CFB400-10?

CFB400-10 is a 400nm carboxyl-functionalized blue fluorescent microsphere suspension with 1% solids content.

What are the excitation and emission wavelengths?

The excitation wavelength is 366nm, and the emission wavelength is 460nm.

Can CFB400-10 be coupled with antibodies?

Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody or protein conjugation.

Is CFB400-10 suitable for lateral flow assays?

It can be evaluated as a fluorescent lateral flow reporter. Membrane pore size, buffer composition, particle migration and optical reader settings should be optimized.

What equipment can detect CFB400-10?

Use a fluorescence reader, microscope or optical system equipped with compatible UV excitation and blue emission filters.

Can it be used for multiplex detection?

Yes. Blue fluorescent microspheres can be combined with particles carrying other fluorescence colors or intensities for multiplex assay research.

Can SHBC provide samples?

Yes. Samples are available for biomolecule coupling, fluorescence evaluation and assay development.

Is bulk production available?

Yes. SHBC supports pilot-scale orders and bulk manufacturing for biotechnology companies, diagnostic developers and research institutions.

Can CFB400-10 be customized?

Yes. Particle size, fluorescence wavelength, surface group, solids content, suspension buffer and packaging can be customized.

Ordering Information

Product Name: 400nm Carboxyl Blue-Fluo Microspheres
Catalog Number: CFB400-10
Particle Size: 400nm
Solids Content: 1%
Excitation Wavelength: 366nm
Emission Wavelength: 460nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

Contact SHBC for samples, technical documents, customized specifications and bulk production quotations.

For research and manufacturing use. Performance in the final application should be validated by the customer.