Product Overview
CFB400-10 Carboxyl Blue Fluorescent Microspheres are premium fluorescent polystyrene nanoparticles developed for fluorescence labeling, biomolecule conjugation and in vitro diagnostic applications. Featuring bright blue fluorescence, highly uniform particle size and reactive surface carboxyl groups, these microspheres deliver reliable performance in fluorescence immunoassays, biosensors and life science research.
Supplied as a 1% aqueous suspension, CFB400-10 provides excellent suspension stability, strong fluorescence intensity and outstanding batch-to-batch consistency. The surface carboxyl groups can be activated using EDC/NHS coupling chemistry, allowing efficient covalent attachment of antibodies, proteins, peptides and other amine-containing biomolecules for fluorescence-based analytical assays.
Key Features
Uniform 400 nm particle size
Bright blue fluorescence
Surface carboxyl functional groups
High fluorescence intensity
Excellent particle size uniformity
Stable aqueous suspension
Compatible with EDC/NHS coupling
High biomolecule conjugation efficiency
Low nonspecific adsorption
Excellent batch-to-batch consistency
Technical Specifications
Parameter | Specification |
|---|
Product Code | CFB400-10 |
Material | Fluorescent Polystyrene Microspheres |
Particle Size | 400 nm |
Surface | Carboxyl (-COOH) |
Solid Content | 1% Suspension |
Excitation (Ex) | 366 nm |
Emission (Em) | 460 nm |
Appearance | Blue Fluorescent Suspension |
Storage | 2–8°C |
Packaging | 10 mL / 20 mL / 100 mL |
Applications
Fluorescence Immunoassays
Lateral Flow Assays (LFA)
Antibody Conjugation
Protein Labeling
Biosensors
Molecular Diagnostics
Fluorescence Calibration
Cell Analysis
In Vitro Diagnostics (IVD)
Life Science Research
Product Advantages
CFB400-10 combines bright blue fluorescence with reactive surface carboxyl groups, providing excellent biomolecule conjugation efficiency and stable fluorescence performance. The 400 nm particle size offers a balance of high fluorescence intensity, excellent suspension stability and large surface area for antibody immobilization, making these microspheres ideal for fluorescence immunoassays, biosensors, lateral flow assays and molecular diagnostic reagent development. Their excellent particle uniformity ensures reliable and reproducible analytical results.
Storage and Handling
Store at 2–8°C.
Do not freeze.
Protect from direct light.
Keep the container tightly sealed.
Gently invert before use.
Avoid repeated freeze-thaw cycles.
Prevent microbial contamination during handling.
Customization Service
SANYU GROUP provides customized fluorescent microspheres including:
Customized particle sizes
Customized fluorescence intensity
Customized excitation and emission wavelengths
Surface functionalization
Customized solid content
OEM manufacturing
Bulk production
Custom packaging
Frequently Asked Questions
What are Carboxyl Blue Fluorescent Microspheres?
Carboxyl Blue Fluorescent Microspheres are fluorescent polystyrene microspheres with reactive carboxyl groups designed for fluorescence labeling, biomolecule conjugation and diagnostic assay development.
What are the excitation and emission wavelengths of CFB400-10?
CFB400-10 has an excitation wavelength of 366 nm and an emission wavelength of 460 nm, producing bright blue fluorescence for fluorescence microscopy, analytical instruments and fluorescence detection systems.
What applications is CFB400-10 suitable for?
CFB400-10 is widely used for fluorescence immunoassays, lateral flow assays, antibody conjugation, protein labeling, biosensors, molecular diagnostics, fluorescence calibration, in vitro diagnostics and life science research.
Can antibodies be conjugated to CFB400-10?
Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry to covalently attach antibodies, proteins, peptides and other amine-containing biomolecules.
How should CFB400-10 be stored?
Store at 2–8°C, protect the suspension from light, avoid freezing, and gently invert before use to maintain uniform particle dispersion and optimal fluorescence performance.
