Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
-
CFG400-10
-
SHBC
-
1%
-
400nm
-
10ml,20ml,50ml ,500ml,1000ml
400nm Carboxyl Green-Fluo Microspheres CFG400-10
SHBC CFG400-10 Carboxyl Green-Fluo Microspheres are 400nm fluorescent polymer microspheres with surface carboxyl groups.
The microspheres provide green fluorescence with excitation at 470/488nm and emission at 525nm. A 1% solids suspension is supplied for fluorescence immunoassay development, quantitative lateral flow testing, biosensing and biomolecule conjugation.
Product Specifications
Item | Specification |
|---|---|
Product Name | Carboxyl Green-Fluo Microspheres |
Catalog Number | CFG400-10 |
Brand | SHBC |
Particle Size | 400nm |
Solids Content | 1% |
Excitation Wavelength | 470/488nm |
Emission Wavelength | 525nm |
Fluorescence Color | Green |
Surface Group | Carboxyl, COOH |
Product Form | Microsphere Suspension |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Key Product Features
Bright green fluorescence at 525nm
Compatible with 470nm and 488nm excitation
Surface carboxyl groups for biomolecule coupling
Uniform 400nm particle size
Good suspension and dispersion performance
Stable fluorescent signal
Suitable for quantitative fluorescence detection
Available for research and batch production
Why Choose 400nm Green Fluorescent Microspheres?
The 400nm particle size provides a practical balance between fluorescent signal intensity, available coupling surface and membrane migration performance.
CFG400-10 is suitable for developing fluorescent probes that require stronger optical signals than smaller nanoparticles while maintaining good dispersion and assay compatibility.
Carboxyl Surface for Biomolecule Coupling
The microsphere surface contains carboxyl functional groups that can be activated for covalent conjugation with primary amine-containing biomolecules.
Compatible biomolecules include:
Antibodies
Antigens
Proteins
Peptides
Enzymes
Oligonucleotides
Other amine-containing ligands
EDC/NHS chemistry can be used to form stable amide bonds between the microspheres and target biomolecules.
Applications
Quantitative Lateral Flow Immunoassays
CFG400-10 can be used as a fluorescent reporter particle in quantitative lateral flow assays for detecting proteins, antibodies, antigens and other biomarkers.
Fluorescence Immunoassays
The microspheres provide a strong green fluorescent signal for sandwich immunoassays, competitive assays and fluorescence-based detection systems.
Point-of-Care Test Development
The 470/488nm excitation and 525nm emission profile is compatible with commonly used green fluorescence detection modules in POCT readers.
Antibody and Protein Conjugation
Surface carboxyl groups support covalent immobilization of antibodies, proteins and other biological ligands for stable fluorescent probe preparation.
Biosensors and Biochips
The microspheres can be used as fluorescent signal carriers in biosensors, microarrays, biochips and surface-based detection platforms.
Particle Tracing and Imaging
Their green fluorescence makes the microspheres suitable for fluorescence microscopy, particle tracking, transport studies and material distribution research.
Advantages for Fluorescent Assay Development
Strong fluorescence improves signal recognition
Carboxyl groups support stable covalent coupling
Uniform particles improve assay reproducibility
Good dispersibility supports consistent membrane migration
488nm excitation is compatible with common optical systems
Suitable for qualitative and quantitative detection
Scalable supply supports product development and manufacturing
Recommended Coupling Process
A typical EDC/NHS coupling procedure includes:
Disperse the microsphere suspension thoroughly.
Wash the microspheres with an appropriate activation buffer.
Activate the surface carboxyl groups using EDC and NHS.
Add the antibody, protein or other amine-containing biomolecule.
Incubate under optimized reaction conditions.
Block the remaining active sites.
Wash and resuspend the conjugated microspheres.
Evaluate fluorescence, coupling efficiency and assay performance.
Coupling conditions should be optimized according to the biomolecule, buffer system and final application.
Recommended Handling
Mix thoroughly before use.
Avoid prolonged high-power ultrasonication.
Protect the suspension from strong light.
Do not allow the microspheres to dry.
Avoid repeated freezing and thawing.
Use clean, low-binding laboratory consumables.
Confirm buffer compatibility before large-scale conjugation.
Quality and Batch Supply
SHBC supports fluorescent microsphere projects from early-stage assay development to batch production.
Available services include:
Different particle sizes
Different surface functional groups
Fluorescence wavelength customization
Solids-content adjustment
Surface carboxyl density optimization
Custom packaging
OEM and ODM production
Research samples and bulk supply
Why Choose SHBC?
Shanghai SanYu Biotechnology Co., Ltd. specializes in functional microspheres and magnetic beads for immunoassays, lateral flow tests, fluorescence detection and bioseparation.
SHBC provides:
Controlled particle-size production
Stable fluorescence performance
Functional surface modification
Reliable batch-to-batch consistency
Technical support for biomolecule coupling
Flexible packaging and bulk manufacturing
Custom microsphere development
Frequently Asked Questions
What is CFG400-10?
CFG400-10 is a 400nm carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.
What are the excitation and emission wavelengths?
The recommended excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.
Can CFG400-10 be coupled with antibodies?
Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry for covalent coupling with antibody amino groups.
Is CFG400-10 suitable for lateral flow assays?
Yes. It is designed for fluorescent lateral flow immunoassay development, including qualitative and quantitative detection.
What detection equipment can be used?
The microspheres can be detected using compatible fluorescence readers, fluorescence microscopes and optical systems equipped with suitable 470nm or 488nm excitation and 525nm emission filters.
Can SHBC provide bulk quantities?
Yes. SHBC supports laboratory samples, pilot-scale evaluation and bulk production for research institutions, diagnostic developers and reagent manufacturers.
Can the particle size or fluorescence wavelength be customized?
Yes. Particle size, fluorescence wavelength, surface functional group, solids content and packaging can be customized according to project requirements.
Ordering Information
Product: 400nm Carboxyl Green-Fluo Microspheres
Catalog Number: CFG400-10
Solids Content: 1%
Excitation: 470/488nm
Emission: 525nm
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Contact SHBC for samples, technical specifications, customization and bulk production quotations.
For research and manufacturing use. Performance in final diagnostic products should be validated by the customer.