400nm Carboxyl Yellow-Fluo Microspheres 1% CFY400-10

400nm Carboxyl Yellow-Fluo Microspheres CFY400-10

SHBC CFY400-10 is a 400nm carboxyl functionalized yellow fluorescent microsphere designed for biomolecule labeling, immunoassay development and fluorescence-based biological detection.

The microspheres provide stable yellow fluorescence with an excitation wavelength of 465nm and an emission wavelength of 510nm.

With reactive carboxyl groups (-COOH) on the surface, CFY400-10 enables covalent coupling with antibodies, proteins, peptides and other biological molecules through EDC/NHS chemistry.

Product Specifications of CFY400-10

Yellow Fluorescence Properties (Ex 465nm / Em 510nm)

CFY400-10 provides yellow fluorescence suitable for fluorescence detection platforms and biological assay development.

Optical characteristics:

• Excitation wavelength: 465nm

• Emission wavelength: 510nm

• Fluorescence color: Yellow

The stable fluorescence signal allows compatibility with fluorescence readers, imaging systems and analytical instruments.

The defined excitation and emission wavelengths help improve detection consistency in research and diagnostic applications.

Carboxyl Functionalized Surface for Biomolecule Coupling

CFY400-10 contains surface carboxyl groups that provide reactive sites for biomolecule immobilization.

The carboxyl groups can be activated using EDC/NHS coupling chemistry to form stable amide bonds with amino groups of proteins and antibodies.

Typical coupling process:

Carboxyl Microsphere Activation → Antibody/Protein Coupling → Blocking → Fluorescent Bioconjugate Formation

Advantages include:

• Stable covalent attachment

• Reduced biomolecule dissociation

• Improved assay reproducibility

• Flexible surface modification

Advantages of 400nm Yellow Fluorescent Microspheres

Controlled 400nm Particle Size

The 400nm particle size provides:

• Good dispersion stability

• High surface area for biomolecule loading

• Reliable optical performance

• Suitable signal intensity

• Consistent assay performance

Carboxyl Functional Surface

The reactive -COOH groups enable:

• Antibody immobilization

• Protein coupling

• Peptide attachment

• Surface modification

• Diagnostic reagent development

Stable Yellow Fluorescence

CFY400-10 provides:

• Stable fluorescence emission

• Good batch-to-batch consistency

• Strong optical signal

• Compatibility with fluorescence detection systems

Applications in Immunoassay and Diagnostic Development

CFY400-10 can be used as a fluorescent carrier for various biological detection applications.

Potential applications include:

• Immunofluorescence assays

• Antibody labeling

• Antigen detection

• Biomarker analysis

• Diagnostic reagent development

• Research assay platforms

The combination of controlled particle size and carboxyl surface chemistry makes CFY400-10 suitable for developing fluorescence-based detection products.

Antibody and Protein Conjugation Using Carboxyl Microspheres

Carboxyl fluorescent microspheres are commonly used for covalent attachment of biological molecules.

Compatible molecules include:

• Monoclonal antibodies

• Polyclonal antibodies

• Antigens

• Recombinant proteins

• Peptides

• Nucleic acids

General coupling workflow:

1. Microsphere preparation

2. Carboxyl group activation

3. Biomolecule addition

4. Covalent coupling reaction

5. Blocking

6. Conjugate performance evaluation

The coupling conditions can be optimized according to the target molecule and assay requirements.

Fluorescent Microspheres for Lateral Flow Assay

CFY400-10 can be evaluated as a fluorescent labeling material for lateral flow assay development.

Potential benefits:

• Higher detection sensitivity compared with visible labels

• Stable fluorescence signal

• Flexible antibody conjugation

• Suitable for fluorescence readers

Applications include:

• POCT development

• Infectious disease detection

• Food safety testing

• Veterinary diagnostics

• Environmental monitoring

Fluorescent Beads for Flow Cytometry and Bioanalysis

CFY400-10 fluorescent microspheres may be used in fluorescence-based analytical systems.

Potential applications include:

• Flow cytometry calibration

• Fluorescent particle standards

• Multiplex assay development

• Bioanalytical research

The controlled fluorescence characteristics support reliable optical detection and instrument compatibility.

Comparison of CFY400-10 with Standard Fluorescent Microspheres

Compared with non-functional fluorescent particles, carboxyl fluorescent microspheres provide additional flexibility for antibody and protein immobilization.

Storage and Handling Recommendations

For maintaining optimal performance:

• Avoid repeated freeze-thaw cycles

• Protect from strong light exposure

• Mix gently before use

• Maintain uniform particle dispersion

• Store according to recommended conditions

Proper handling helps preserve particle stability and fluorescence intensity.

Custom Fluorescent Microsphere Manufacturing

Shanghai SanYu Biotechnology Co., Ltd. provides customized fluorescent microsphere solutions.

Available customization options include:

• Particle size adjustment

• Different fluorescence colors

• Surface functional groups

• Solid content adjustment

• Bulk manufacturing

SHBC fluorescent microsphere products include:

• Carboxyl fluorescent microspheres

• Amino fluorescent microspheres

• Magnetic fluorescent beads

• Standard fluorescent particles

Frequently Asked Questions

What are CFY400-10 microspheres?

CFY400-10 are 400nm carboxyl functionalized yellow fluorescent microspheres designed for biomolecule coupling and fluorescence detection.

What are the excitation and emission wavelengths?

The excitation wavelength is 465nm and emission wavelength is 510nm.

What functional group is available on CFY400-10?

The microsphere surface contains carboxyl (-COOH) groups.

Can CFY400-10 be used for antibody conjugation?

Yes. The carboxyl groups support antibody and protein coupling through EDC/NHS activation chemistry.

What applications use yellow fluorescent microspheres?

They are commonly used in immunoassays, fluorescence labeling, lateral flow assays and bioanalysis.

What is the solid content?

The standard solid content is 1%.

Can SHBC supply bulk quantities?

Yes. Shanghai SanYu Biotechnology Co., Ltd. provides laboratory, pilot-scale and bulk supply of fluorescent microspheres.

Request CFY400-10 Samples and Bulk Pricing

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical information and bulk quotations of 400nm Carboxyl Yellow-Fluo Microspheres CFY400-10.

CFY400-10 combines controlled 400nm particle size, stable yellow fluorescence and carboxyl surface chemistry for advanced immunoassay and fluorescence detection applications.