40um Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB40UM 10%

40 μm Carboxyl Crosslinked PS/DVB Microspheres CPS/DVB40UM

SHBC CPS/DVB40UM Carboxyl Crosslinked PS/DVB Microspheres are 40 μm functional polymer microspheres with a crosslinked polystyrene/divinylbenzene matrix and reactive surface carboxyl groups.

Supplied at 10% solids, CPS/DVB40UM is designed for antibody coupling, protein immobilization, bead-based immunoassays and custom functional particle development.

The larger 40 μm particle format provides convenient handling, washing and recovery for research and scalable reagent production.

CPS/DVB40UM Technical Specifications

Crosslinked PS/DVB Structure

CPS/DVB40UM uses divinylbenzene to crosslink the polystyrene matrix.

The crosslinked polymer network provides a rigid and stable particle structure suitable for larger microsphere formats and repeated processing.

Commercial suppliers also use DVB crosslinking for larger polystyrene microspheres.

Key benefits include:

• Stable spherical morphology

• Good particle rigidity

• Suitable for repeated washing

• Reliable particle recovery

• Suitable for surface functionalization

• Convenient handling in bead-based assays

Carboxyl Surface for Covalent Coupling

Surface carboxyl groups provide reactive sites for covalent attachment of amine-containing biomolecules.

Typical coupling chemistry:

COOH Surface → EDC/NHS Activation → Amine-Containing Biomolecule → Stable Amide Bond

Carboxyl-functional PS and PS/DVB microspheres are commonly used for EDC/EDAC-mediated immobilization of proteins, peptides and amino-functional biomolecules.

Key Advantages of CPS/DVB40UM

• 40 μm particle size

• High 10% solid content

• Crosslinked PS/DVB matrix

• Reactive carboxyl surface

• Suitable for EDC/NHS coupling

• Stable covalent ligand attachment

• Convenient washing and recovery

• Easy particle handling

• Suitable for antibodies and antigens

• Suitable for research and bulk production

Why Choose 40 μm Carboxyl Microspheres?

The 40 μm particle format is suitable for applications requiring a larger and easily recoverable functional solid phase.

Potential advantages include:

• Easy particle sedimentation

• Convenient centrifugation

• Straightforward washing

• Easy visual monitoring

• Reduced particle loss during processing

• Convenient batch coupling

• Easy resuspension

• Suitable for larger-volume workflows

Commercial crosslinked carboxyl polystyrene particles are available in the approximately 40–45 μm size range, confirming this particle class as an established functional microsphere format.

Immunoassay Applications

Antibody-Coupled Microspheres

Capture antibodies can be covalently immobilized on CPS/DVB40UM for:

• Bead-based immunoassays

• Antigen detection

• Biomarker detection

• Serological assay development

• Pathogen-detection research

• Custom diagnostic reagent development

Covalent immobilization is useful when stable ligand retention during washing and storage is required.

Antigen-Coupled Microspheres

Antigens can be immobilized for:

• Antibody detection

• Serology research

• Autoantibody assays

• Infectious-disease research

• Assay development

• Method validation

Protein and Biomolecule Applications

CPS/DVB40UM can also be evaluated for:

• Protein immobilization

• Enzyme immobilization

• Peptide coupling

• Affinity ligand preparation

• Biomolecule capture

• Biosensor development

• Surface chemistry research

• Oligonucleotide immobilization

• Custom functional microspheres

Suitable Coupling Molecules

The carboxyl-functional surface can be evaluated with:

• Monoclonal antibodies

• Polyclonal antibodies

• Recombinant proteins

• Diagnostic antigens

• Peptides

• Enzymes

• Amino-modified DNA

• Amino-modified RNA

• Other amine-containing ligands

Final coupling performance depends on ligand purity, concentration and reaction conditions.

General EDC/NHS Coupling Workflow

1. Resuspend the Microspheres

Mix CPS/DVB40UM thoroughly before sampling.

2. Wash the Microspheres

Wash the particles using a suitable activation buffer.

3. Activate Carboxyl Groups

Add EDC with or without NHS to activate surface carboxyl groups.

4. Add the Biomolecule

Add the antibody, antigen, protein or other amine-containing ligand.

5. Perform Covalent Coupling

Allow activated carboxyl groups to react with primary amino groups on the biomolecule.

6. Block Residual Reactive Sites

Use a suitable blocking or quenching reagent after coupling.

7. Wash and Resuspend

Remove unbound biomolecules and coupling reagents, then resuspend the functionalized microspheres in the selected assay or storage buffer.

EDC-mediated coupling is an established method for covalent immobilization on carboxyl-functionalized polymer microspheres.

Factors Affecting Coupling Efficiency

Important optimization parameters include:

• Microsphere concentration

• Ligand concentration

• EDC concentration

• NHS concentration

• Activation pH

• Coupling-buffer composition

• Reaction temperature

• Incubation time

• Mixing efficiency

• Blocking conditions

Small-scale optimization is recommended before production scale-up.

Immunoassay Development Tips

For consistent assay performance:

• Use purified antibodies or antigens.

• Avoid unnecessary carrier proteins.

• Keep particles uniformly suspended.

• Optimize EDC/NHS concentration.

• Control activation pH.

• Optimize ligand loading.

• Block residual reactive sites.

• Evaluate nonspecific binding.

• Test coupled-particle stability.

• Verify batch-to-batch performance.

Because 40 μm microspheres settle faster than smaller particles, continuous or periodic mixing is recommended during coupling and assay incubation.

CPS/DVB40UM vs. Smaller Carboxyl Microspheres

The preferred size depends on ligand capacity, assay kinetics, washing method and detection system.

Crosslinked PS/DVB vs. Standard PS Microspheres

Crosslinked PS/DVB is suitable when both particle stability and functional surface chemistry are required.

Covalent Coupling vs. Passive Adsorption

Quality Control for Bulk Production

For immunoassay reagent development, evaluate:

• Particle-size consistency

• Suspension uniformity

• Solid-content consistency

• Surface carboxyl functionality

• Coupling efficiency

• Ligand loading

• Nonspecific binding

• Signal-to-background ratio

• Assay sensitivity

• Storage stability

• Batch-to-batch reproducibility

Why Choose SHBC CPS/DVB40UM?

• 40 μm carboxyl crosslinked PS/DVB microspheres

• High 10% solid content

• Reactive COOH surface

• Suitable for EDC/NHS coupling

• Designed for antibody and protein immobilization

• Suitable for immunoassay development

• Samples available for evaluation

• Stable batch manufacturing

• Bulk production capability

• Support for research and production quantities

Storage and Handling

Recommended practices include:

• Keep the container tightly sealed.

• Mix thoroughly before sampling.

• Resuspend settled microspheres before use.

• Avoid unnecessary freezing.

• Prevent microbial contamination.

• Use clean pipettes and containers.

• Avoid conditions causing irreversible aggregation.

• Validate storage conditions after biomolecule coupling.

Frequently Asked Questions

What is CPS/DVB40UM?

CPS/DVB40UM is a 40 μm carboxyl-functional crosslinked PS/DVB microsphere product supplied at 10% solids.

What does PS/DVB mean?

PS/DVB refers to polystyrene crosslinked with divinylbenzene.

Why use a crosslinked PS/DVB structure?

Crosslinking creates a more rigid polymer network and improves structural stability for larger microsphere formats.

What is the function of the carboxyl surface?

Surface carboxyl groups provide reactive sites for covalent attachment of antibodies, proteins and other amine-containing biomolecules.

Can antibodies be coupled to CPS/DVB40UM?

Yes. Antibodies can be immobilized using optimized EDC or EDC/NHS coupling chemistry.

Can CPS/DVB40UM be used for immunoassays?

Yes. It can be evaluated for bead-based antibody, antigen and biomarker-detection applications.

What is the solid content?

CPS/DVB40UM is supplied at 10% solids.

Can amino-modified oligonucleotides be coupled?

Yes. Amino-modified DNA or RNA can be evaluated for carbodiimide-mediated coupling to the carboxyl surface.

Why choose 40 μm microspheres?

40 μm particles provide convenient handling, washing and recovery. Smaller particles generally provide greater surface area per unit mass.

Do 40 μm microspheres require mixing?

Yes. Larger microspheres settle more rapidly, so periodic or continuous mixing is recommended during coupling and assay reactions.

Is coupling optimization necessary?

Yes. EDC/NHS concentration, activation pH, ligand loading, reaction time and blocking conditions should be optimized for each project.

Can SHBC supply bulk quantities?

Yes. SHBC supports sample evaluation and bulk supply for immunoassay developers, biotechnology companies and research organizations.

Request CPS/DVB40UM Samples

Contact Shanghai SanYu Biotechnology Co., Ltd. for CPS/DVB40UM samples, technical documentation and bulk quotations.

Please provide:

• Target antibody or protein

• Assay format

• Coupling chemistry

• Required microsphere quantity

• Target application

• Development stage

• Estimated annual demand

CPS/DVB40UM is supplied for research and process development. Final diagnostic applications should be validated by the customer.