Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
-
CFG500-10
-
SHBC
-
1%
-
500nm
-
10ml,20ml,50ml ,500ml,1000ml
500nm Carboxyl Green-Fluo Microspheres CFG500-10
SHBC CFG500-10 Carboxyl Green-Fluo Microspheres are 500nm fluorescent polymer microspheres with carboxyl-functionalized surfaces.
The microspheres provide bright green fluorescence with excitation at 470/488nm and emission at 525nm. They are supplied as a 1% solids suspension for fluorescent immunoassays, quantitative lateral flow tests, biosensors and biomolecule conjugation.
Product Specifications
Item | Specification |
|---|---|
Product Name | Carboxyl Green-Fluo Microspheres |
Catalog Number | CFG500-10 |
Brand | SHBC |
Particle Size | 500nm / 0.5μm |
Solids Content | 1% |
Excitation Wavelength | 470/488nm |
Emission Wavelength | 525nm |
Fluorescence Color | Green |
Surface Functional Group | Carboxyl, COOH |
Product Form | Microsphere Suspension |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Key Features
Bright green fluorescent signal
Compatible with 470nm and 488nm excitation
Strong emission at 525nm
Carboxyl surface for covalent biomolecule coupling
Uniform 500nm particle size
Good suspension and dispersion performance
Suitable for qualitative and quantitative assays
Available for research and batch production
Why Choose 500nm Green Fluorescent Microspheres?
The 500nm particle size provides a large fluorescent signal carrier and sufficient surface area for biomolecule immobilization.
CFG500-10 is suitable for assay systems requiring:
Strong fluorescent signal output
Stable antibody or protein conjugation
Reliable particle recognition
Consistent quantitative detection
Good compatibility with fluorescence readers
The 0.5μm particle size is particularly useful for fluorescent lateral flow assays, biosensors and fluorescence-based particle detection.
Carboxyl Surface for Covalent Coupling
CFG500-10 contains carboxyl groups on the microsphere surface. These groups can be activated for covalent conjugation with primary amine-containing biomolecules.
Compatible ligands include:
Antibodies
Antigens
Proteins
Peptides
Enzymes
Oligonucleotides
Other amine-containing molecules
EDC/NHS chemistry can be used to form stable amide bonds between the microspheres and the target biomolecule.
Main Applications
Quantitative Lateral Flow Immunoassays
CFG500-10 can be used as a fluorescent reporter particle in quantitative lateral flow immunoassays.
The bright 525nm emission supports sensitive signal detection using compatible fluorescence readers.
Fluorescence Immunoassays
The microspheres are suitable for sandwich immunoassays, competitive immunoassays and other fluorescence-based detection platforms.
Applications include the detection of:
Proteins
Antibodies
Antigens
Hormones
Pathogen biomarkers
Inflammatory markers
Other biological analytes
Point-of-Care Test Development
The 470/488nm excitation profile is compatible with commonly used green fluorescence optical modules.
CFG500-10 can be evaluated for portable fluorescence readers and point-of-care testing systems.
Antibody and Protein Conjugation
The carboxyl-functionalized surface supports stable covalent immobilization of antibodies, proteins and peptides.
It can be used to prepare fluorescent probes for target capture, recognition and signal amplification.
Biosensors and Biochips
CFG500-10 can serve as a fluorescent signal carrier in:
Biosensors
Microarrays
Biochips
Microfluidic systems
Surface-based detection platforms
Multiplex detection research
Flow Cytometry and Fluorescence Imaging
The microspheres can be evaluated for fluorescence instrument calibration, particle tracking, fluorescence microscopy and flow-based particle analysis.
Cell and Particle Tracking
The green fluorescent signal allows the microspheres to be detected using suitable optical equipment.
They may be used in particle transport, material distribution, cell uptake and tracing studies.
Advantages for Assay Development
Strong Fluorescent Signal
The 500nm particle size can carry a strong fluorescence signal for optical detection and quantitative analysis.
Stable Covalent Conjugation
Surface carboxyl groups enable covalent coupling with amino-containing biomolecules through established activation chemistry.
Good Assay Reproducibility
Controlled particle size and consistent fluorescence help improve test repeatability and batch comparison.
Flexible Platform Compatibility
The 470/488nm excitation and 525nm emission profile is suitable for many green fluorescence detection systems.
Research-to-Production Supply
SHBC supports product evaluation, formulation development, pilot-scale testing and batch manufacturing.
Recommended Coupling Workflow
A typical EDC/NHS coupling process includes:
Mix the microsphere suspension thoroughly.
Wash the microspheres with a suitable activation buffer.
Activate the surface carboxyl groups using EDC and NHS.
Add the antibody, protein or other amine-containing ligand.
Incubate under optimized reaction conditions.
Block the remaining active sites.
Wash the conjugated microspheres.
Resuspend the microspheres in a suitable storage buffer.
Evaluate coupling efficiency and assay performance.
The reagent concentration, pH, reaction time and blocking conditions should be optimized for each biomolecule and application.
Handling Recommendations
Mix thoroughly before sampling.
Protect the product from strong light.
Avoid prolonged high-power ultrasonication.
Prevent the microspheres from drying.
Avoid repeated freezing and thawing.
Use clean and low-binding laboratory consumables.
Verify buffer compatibility before large-scale coupling.
Follow the product label and batch documentation for storage requirements.
Customization and Bulk Supply
Shanghai SanYu Biotechnology Co., Ltd. provides fluorescent microspheres for research institutions, diagnostic developers and reagent manufacturers.
Available customization services include:
Different particle sizes
Different fluorescence wavelengths
Carboxyl density adjustment
Alternative surface functional groups
Solids-content adjustment
Buffer formulation
Custom packaging
OEM and ODM production
Pilot-scale and bulk manufacturing
Why Choose SHBC?
SHBC specializes in functional microspheres and magnetic beads for immunoassays, lateral flow diagnostics, fluorescence detection and bioseparation.
Our advantages include:
Controlled particle-size production
Stable fluorescence performance
Functional surface modification
Reliable batch-to-batch consistency
Technical support for biomolecule coupling
Flexible packaging options
Research samples and bulk quantities
Custom microsphere development
Frequently Asked Questions
What is CFG500-10?
CFG500-10 is a 500nm carboxyl-functionalized green fluorescent microsphere suspension with 1% solids content.
What are its excitation and emission wavelengths?
The recommended excitation wavelengths are 470nm and 488nm. The emission wavelength is 525nm.
Can CFG500-10 be coupled with antibodies?
Yes. Its surface carboxyl groups can be activated using EDC/NHS chemistry for covalent antibody conjugation.
Is CFG500-10 suitable for lateral flow assays?
Yes. It can be evaluated as a fluorescent reporter particle for qualitative and quantitative lateral flow immunoassays.
What is the difference between 500nm and smaller fluorescent microspheres?
The 500nm particles provide a larger fluorescent carrier and more surface area per particle. Final sensitivity and membrane migration should be optimized according to the assay design.
What equipment can detect these microspheres?
They can be detected using compatible fluorescence readers, fluorescence microscopes and optical systems equipped with suitable excitation and emission filters.
Can SHBC provide samples?
Yes. Samples are available for coupling evaluation, formulation development and assay optimization.
Is bulk production available?
Yes. SHBC supports pilot-scale orders and bulk manufacturing for diagnostic reagent developers and industrial customers.
Can SHBC customize the particle size or wavelength?
Yes. Particle size, fluorescence wavelength, surface functional group, solids content, buffer and packaging can be customized.
Ordering Information
Product Name: 500nm Carboxyl Green-Fluo Microspheres
Catalog Number: CFG500-10
Particle Size: 500nm / 0.5μm
Solids Content: 1%
Excitation Wavelength: 470/488nm
Emission Wavelength: 525nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Contact SHBC for samples, technical documents, customization services and bulk production quotations.
For research and manufacturing use. Performance in final diagnostic products should be validated by the customer.