Product Overview
CFG500-10 Carboxyl Green Fluorescent Microspheres are premium fluorescent polystyrene nanoparticles designed for fluorescence labeling, biomolecule conjugation and in vitro diagnostic applications. Featuring bright green fluorescence, highly uniform particle size and reactive surface carboxyl groups, these microspheres provide reliable performance for fluorescence immunoassays, biosensors and life science research.
Supplied as a 1% aqueous suspension, CFG500-10 offers excellent suspension stability, high fluorescence intensity and outstanding batch-to-batch consistency. The surface carboxyl groups can be activated using EDC/NHS coupling chemistry, enabling efficient covalent conjugation of antibodies, proteins, peptides and other amine-containing biomolecules.
Key Features
Uniform 500 nm particle size
Bright green fluorescence
Surface carboxyl functional groups
High fluorescence intensity
Excellent particle size uniformity
Stable aqueous suspension
Compatible with EDC/NHS coupling
High biomolecule conjugation efficiency
Low nonspecific adsorption
Excellent batch-to-batch consistency
Technical Specifications
Parameter | Specification |
|---|
Product Code | CFG500-10 |
Material | Fluorescent Polystyrene Microspheres |
Particle Size | 500 nm |
Surface | Carboxyl (-COOH) |
Solid Content | 1% Suspension |
Excitation (Ex) | 470/488 nm |
Emission (Em) | 525 nm |
Appearance | Green Fluorescent Suspension |
Storage | 2–8°C |
Packaging | 10 mL / 20 mL / 100 mL |
Applications
Fluorescence Immunoassays
Lateral Flow Assays (LFA)
Antibody Conjugation
Protein Labeling
Biosensors
Molecular Diagnostics
Fluorescence Calibration
Cell Analysis
In Vitro Diagnostics (IVD)
Life Science Research
Product Advantages
CFG500-10 combines bright green fluorescence with reactive surface carboxyl groups, providing excellent biomolecule conjugation efficiency and stable fluorescence performance. The 500 nm particle size offers excellent suspension stability, high fluorescence brightness and abundant surface area for antibody immobilization, making these microspheres ideal for fluorescence immunoassays, biosensors, lateral flow assays and molecular diagnostic reagent development. Their uniform particle size and consistent fluorescence ensure reliable and reproducible analytical performance.
Storage and Handling
Store at 2–8°C.
Do not freeze.
Protect from direct light.
Keep the container tightly sealed.
Gently invert before use.
Avoid repeated freeze-thaw cycles.
Prevent microbial contamination during handling.
Customization Service
SANYU GROUP provides customized fluorescent microspheres including:
Customized particle sizes
Customized fluorescence intensity
Customized excitation and emission wavelengths
Surface functionalization
Customized solid content
OEM manufacturing
Bulk production
Custom packaging
Frequently Asked Questions
What are Carboxyl Green Fluorescent Microspheres?
Carboxyl Green Fluorescent Microspheres are fluorescent polystyrene microspheres with reactive carboxyl groups designed for fluorescence labeling, biomolecule conjugation and diagnostic assay development.
What are the excitation and emission wavelengths of CFG500-10?
CFG500-10 has excitation wavelengths of 470 nm and 488 nm and an emission wavelength of 525 nm, producing bright green fluorescence suitable for fluorescence microscopy, flow cytometry and analytical detection.
What applications is CFG500-10 suitable for?
CFG500-10 is widely used for fluorescence immunoassays, lateral flow assays, antibody conjugation, protein labeling, biosensors, molecular diagnostics, fluorescence calibration, in vitro diagnostics and life science research.
Can antibodies be conjugated to CFG500-10?
Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry to covalently attach antibodies, proteins, peptides and other amine-containing biomolecules.
How should CFG500-10 be stored?
Store at 2–8°C, protect the suspension from light, avoid freezing, and gently invert before use to maintain uniform particle dispersion and optimal fluorescence performance.
