500nm Carboxyl Photonic Crystal Microspheres SCP500NM-10 5%

500nm Carboxyl-Modified Microspheres for Photonic Crystals

SHBC SCP500NM-10 Special Standard Microspheres are 500 nm particles with surface carboxyl groups and a solid content of 5%.

The controlled particle size supports colloidal self-assembly and photonic crystal development. The carboxyl-functionalized surface provides reactive sites for antibody, protein, peptide and biomolecule immobilization.

SCP500NM-10 is suitable for photonic crystal research, optical sensing, biosensor development and immunoassay reagent production.

Product Specifications

Particle matrix, dispersion medium, particle size distribution, refractive index and carboxyl content should be confirmed in the batch-specific specification.

Key Features and Advantages

Controlled 500nm Particle Size

The 500 nm particle size is suitable for colloidal array formation, optical material development and particle-based assay research.

Reactive Carboxyl Surface

Surface carboxyl groups can be activated for covalent coupling with antibodies, antigens, proteins, peptides and amino-modified molecules.

Dual Optical and Biological Functions

SCP500NM-10 combines photonic crystal building-block properties with a biofunctional surface for molecular recognition and assay development.

Convenient 5% Suspension

The 5% solid content supports concentration adjustment, self-assembly testing, coupling optimization and pilot-scale formulation.

Stable Bulk Supply

SHBC supports laboratory samples, customized specifications, pilot orders and industrial batch production.

Photonic Crystal and Self-Assembly Applications

SCP500NM-10 can be evaluated for:

• Colloidal photonic crystal films

• Two-dimensional particle arrays

• Three-dimensional colloidal crystals

• Opal and inverse-opal templates

• Optical reflection studies

• Photonic bandgap research

• Refractive index sensing

• Structural optical materials

• Microfluidic optical sensors

• Nanostructured coatings

• Colloidal self-assembly research

• Particle size standard research

Uniform polymer microspheres are widely used as building blocks for self-assembled photonic crystal structures. Particle size distribution, surface charge and processing conditions strongly influence the quality of the ordered array.

Carboxyl Surface for Biomolecule Coupling

The surface carboxyl groups can be used to immobilize:

• Antibodies

• Antigens

• Recombinant proteins

• Peptides

• Enzymes

• Amino-modified oligonucleotides

• Affinity ligands

• Other amine-containing biomolecules

EDC, commonly combined with NHS or Sulfo-NHS, can activate the surface carboxyl groups. The activated groups react with primary amines to form stable amide bonds.

Carboxyl-functionalized microspheres are widely used as covalent carriers in immunoassay reagent development.

Immunoassay and Biosensor Applications

SCP500NM-10 can be evaluated for:

• Antibody conjugation

• Antigen immobilization

• Optical immunoassays

• Photonic crystal biosensors

• Particle-capture assays

• Latex agglutination research

• Immunoturbidimetric assay development

• Fluorescence-enhanced detection

• Affinity capture

• Multiplex assay development

• Microfluidic diagnostic systems

• Diagnostic reagent raw material research

Photonic crystal arrays can be combined with antibody-modified surfaces and optical detection to develop sensitive biomarker assays.

Why Choose 500nm Microspheres?

The 500 nm particle size provides a balance between optical structure formation and biomolecule-loading capacity.

Key benefits include:

• Suitable for colloidal self-assembly

• Larger coupling surface than coarse particles

• Easier recovery than smaller nanoparticles

• Reactive carboxyl surface

• Suitable for optical sensor development

• Compatible with particle-based immunoassays

• Convenient concentration adjustment

• Suitable for research-to-production scale-up

Final optical and immunoassay performance depends on the particle matrix, size distribution, surface charge, buffer and processing method.

Photonic Crystal Processing Guidance

SCP500NM-10 can be evaluated using:

• Vertical deposition

• Drop casting

• Spin coating

• Evaporation-induced self-assembly

• Air–water interface assembly

• Template-assisted assembly

• Layer-by-layer deposition

Important processing parameters include:

• Microsphere concentration

• Particle size distribution

• Surface charge

• Dispersion stability

• Substrate cleanliness

• Temperature and humidity

• Evaporation rate

• Ionic strength

• Surfactant concentration

• Film thickness

Carboxyl density can affect particle interactions, water retention and film formation. The self-assembly conditions should be optimized for the required optical structure.

Recommended Coupling Guidance

A typical EDC/NHS coupling study may include:

1. Wash and redisperse the microspheres.

2. Transfer them into an amine-free activation buffer.

3. Add freshly prepared EDC and NHS or Sulfo-NHS.

4. Activate the surface carboxyl groups.

5. Add the antibody, antigen or target protein.

6. Incubate under gentle mixing.

7. Block remaining reactive sites.

8. Wash the conjugated microspheres.

9. Resuspend in a suitable storage buffer.

Coupling reagent concentration, pH, protein loading and reaction time should be optimized for each biomolecule.

Recommended Handling

• Mix gently before sampling.

• Fully redisperse particles after standing.

• Avoid contamination from dust or large particles.

• Do not allow the suspension to dry.

• Avoid freezing unless permitted by the specification.

• Use clean substrates for photonic crystal preparation.

• Avoid amine-containing buffers during EDC/NHS activation.

• Control temperature and humidity during film formation.

• Optimize centrifugation before large-scale washing.

• Verify buffer compatibility before production.

Customization and Bulk Production

Shanghai SanYu Biotechnology Co., Ltd. provides microsphere development and manufacturing services for optical material companies, biotechnology companies and diagnostic reagent manufacturers.

Customization options may include:

• Different particle sizes

• Different solid contents

• Narrow particle size distribution

• Carboxyl group density adjustment

• Custom dispersion media

• Fluorescent modification

• Antibody or protein conjugation

• Custom packaging

• Pilot-scale production

• Bulk manufacturing

• OEM and ODM services

Customization feasibility depends on the required specification, application and order volume.

Why Choose SHBC?

• Specialized microsphere manufacturer

• Controlled 500 nm particle specification

• Reactive carboxyl surface

• Standard 5% solid content

• Suitable for photonic crystal research

• Supports antibody and protein coupling

• Laboratory samples available

• Pilot production support

• Stable batch manufacturing

• Custom surface chemistry

• Bulk supply capability

• OEM and ODM support

Quality Documentation

Available documentation may include:

• Certificate of Analysis

• Safety Data Sheet

• Product specification

• Particle size information

• Solid-content information

• Surface group information

• Batch identification

• Storage and handling guidance

Please confirm the available particle distribution, morphology and optical data before placing a bulk order.

Frequently Asked Questions

What is SCP500NM-10?

SCP500NM-10 is a 500 nm carboxyl-modified microsphere suspension with 5% solids for photonic crystal, biosensor and immunoassay research.

What is the function of the carboxyl surface?

The carboxyl groups provide reactive sites for covalent immobilization of antibodies, proteins, peptides and other amino-containing biomolecules.

Can SCP500NM-10 form photonic crystal films?

The microspheres can be evaluated for colloidal self-assembly. Final film quality depends on particle uniformity, concentration, surface charge and deposition conditions.

Can the product be used for immunoassays?

Yes. It can be evaluated for antibody conjugation, particle-capture assays, optical biosensors, agglutination and immunoturbidimetric research.

Which coupling method can be used?

EDC/NHS or EDC/Sulfo-NHS chemistry can be evaluated for coupling biomolecules to the carboxyl surface.

Does carboxyl modification affect self-assembly?

Yes. Surface carboxyl groups can influence particle charge, hydrophilicity and interparticle interactions. Processing conditions should be optimized.

Can SHBC customize the specification?

Yes. Particle size, solid content, carboxyl density, dispersion medium and packaging can be customized according to project requirements.

Are bulk quantities available?

Yes. SHBC supports sample evaluation, pilot orders and bulk production.

Is SCP500NM-10 a finished diagnostic reagent?

No. It is a raw material for research and reagent development. Customers must complete application-specific validation.

Request Samples and Bulk Pricing

Contact SHBC for SCP500NM-10 samples, technical specifications, customized surfaces and bulk quotations.

Please provide:

• Required quantity

• Target optical or immunoassay application

• Required particle size tolerance

• Preferred carboxyl density

• Dispersion medium requirements

• Packaging requirements

• Expected annual demand

Product: 500nm Carboxyl-Modified Special Standard Microspheres for Photonic Crystals
Catalog Number: SCP500NM-10
Particle Size: 500 nm
Solid Content: 5%
Surface Group: Carboxyl, –COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
Applications: Photonic Crystals, Biosensors and Immunoassay Research
Intended Use: Research and Reagent Development Use