Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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CFR500-10
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SHBC
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500nm
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10ml,20ml,50ml,100ml,1000ml
500nm Carboxyl Red-Fluo Microspheres CFR500-10
SHBC CFR500-10 are 500 nm carboxyl-functionalized red fluorescent microspheres developed for fluorescent lateral flow assays, immunoassays and biomolecule labeling.
The microspheres feature excitation at 535 nm, emission at 610 nm and a 1% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and other amino-containing biomolecules.
Product Specifications
Parameter | Specification |
|---|---|
Product Name | Carboxyl Red-Fluo Microspheres |
Catalog Number | CFR500-10 |
Nominal Particle Size | 500 nm |
Solid Content | 1% |
Surface Group | Carboxyl, COOH |
Excitation Wavelength | 535 nm |
Emission Wavelength | 610 nm |
Fluorescence Color | Red |
Supply Form | Aqueous Suspension |
Brand | SHBC |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Batch-specific particle size, size distribution, carboxyl content and fluorescence data are available in the Certificate of Analysis.
Red Fluorescence at 535/610nm
CFR500-10 can be excited at approximately 535 nm and produces red fluorescence centered at approximately 610 nm.
The fluorescence profile is suitable for compatible fluorescence readers, imaging systems and quantitative immunoassay platforms.
Key Advantages
Bright Red Fluorescent Signal
The 535/610 nm optical profile provides a clear red signal for qualitative and quantitative detection.
500nm Particle Size
The 500 nm particle size provides a large fluorescent particle surface for biomolecule immobilization and signal generation.
Reactive Carboxyl Surface
Surface COOH groups support covalent coupling of antibodies, antigens, proteins, peptides and other amino-containing molecules.
Uniform Particle Size
Controlled particle sizing supports repeatable conjugation, membrane migration and assay performance.
1% Aqueous Suspension
The supplied suspension is convenient for laboratory evaluation, coupling development and production scale-up.
Custom and Bulk Supply
SHBC supports samples, customized specifications, pilot production and industrial batch supply.
Main Applications
Fluorescent Lateral Flow Immunoassays
CFR500-10 can be used as a fluorescent reporter particle in qualitative and quantitative lateral flow test strips.
Fluorescence Immunoassays
Suitable for sandwich, competitive and indirect fluorescent immunoassay formats.
Antibody Conjugation
The carboxyl surface supports stable covalent immobilization of antibodies used for target recognition.
Antigen and Protein Coupling
Suitable for coupling antigens, recombinant proteins, enzymes, peptides and other amino-containing biomolecules.
Infectious Disease Testing
Can be evaluated in fluorescent assays for viral, bacterial and other infectious disease biomarkers.
Cardiac and Inflammation Biomarkers
Suitable for assay development involving cardiac markers, inflammatory proteins and low-concentration analytes.
Food and Veterinary Testing
Can be used in fluorescent detection systems for food safety, veterinary diagnostics and agricultural testing.
Fluorescent Tracing
Suitable for selected membrane-flow, filtration, particle-transport and imaging studies.
Advantages in Lateral Flow Assays
CFR500-10 provides:
Bright red fluorescence
High signal per particle
Active COOH coupling surface
Compatibility with quantitative readers
Stable biomolecule immobilization
Suitable size for high-signal test development
Potential for multiplex detection
Support for research and batch production
Because 500 nm particles are relatively large for membrane-based assays, membrane pore structure, buffer composition and conjugate release should be optimized.
Biomolecule Coupling
Surface carboxyl groups can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.
A typical workflow includes:
Wash and disperse the microspheres.
Activate the carboxyl groups with EDC and NHS.
Add the antibody or target protein.
Block remaining active sites.
Wash and resuspend the conjugate.
Evaluate coupling efficiency and assay performance.
Activation pH, protein concentration, reaction time and blocking conditions should be optimized for each biomolecule.
Assay Development Considerations
Important parameters include:
Antibody-to-particle ratio
EDC and NHS concentration
Activation buffer and pH
Coupling time
Blocking reagent
Storage buffer
Membrane pore structure
Conjugate pad treatment
Running buffer composition
Reader filter settings
Optimization is recommended before production scale-up.
Membrane Flow Optimization
The 500 nm particle size can provide strong fluorescence but may migrate more slowly than smaller fluorescent particles.
Evaluate:
Nitrocellulose membrane type
Membrane pore structure
Particle aggregation
Sample viscosity
Surfactant concentration
Salt concentration
Conjugate pad release
Test-line capture density
Background fluorescence
Assay running time
Uniform dispersion is important for stable membrane migration and low background.
Dispersion and Handling
Before use:
Mix by gentle inversion or controlled vortexing
Avoid excessive bubbles and foam
Prevent the microspheres from drying
Protect the product from strong light
Avoid repeated freeze-thaw cycles
Confirm buffer compatibility
Redisperse settled particles before sampling
Controlled bath sonication may be used when necessary to redisperse the microspheres.
Custom Carboxyl Fluorescent Microspheres
SHBC can customize fluorescent microspheres according to assay requirements.
Customization options may include:
Custom particle size
Custom excitation and emission wavelengths
Different fluorescence colors
Carboxyl group density
Solid content
Dispersion buffer
Surface modification
Custom concentration
Custom packaging
OEM and ODM production
Please provide the target application, required wavelength, particle size and estimated quantity when requesting customization.
Quality Control and Documents
Available product documents may include:
Certificate of Analysis
Technical Data Sheet
Safety Data Sheet
Particle size report
Particle size distribution data
Fluorescence spectrum
Carboxyl content data
Microscopy images
Batch inspection report
Why Choose SHBC?
Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.
SHBC provides:
Controlled microsphere manufacturing
Stable particle size distribution
Multiple fluorescence wavelengths
Functional surface modification
Batch-to-batch consistency
Sample evaluation support
Pilot and bulk production
OEM and ODM services
Frequently Asked Questions
What is CFR500-10?
CFR500-10 is a 500 nm carboxyl-functionalized red fluorescent microsphere suspension with 1% solid content.
What are the excitation and emission wavelengths?
The excitation wavelength is 535 nm and the emission wavelength is 610 nm.
Is CFR500-10 suitable for lateral flow assays?
Yes. It can be evaluated as a fluorescent reporter in qualitative and quantitative lateral flow immunoassays.
What can be coupled to CFR500-10?
Antibodies, antigens, proteins, peptides and other molecules containing primary amine groups can be coupled to the carboxyl surface.
Which coupling chemistry is recommended?
EDC/NHS chemistry is commonly used to activate carboxyl groups for covalent protein coupling.
What is the difference between 400nm and 500nm particles?
The 500 nm particles may provide higher signal per particle and more coupling surface, while the 400 nm particles may provide faster membrane migration. Actual performance should be compared in the final assay.
Can a 488nm laser excite CFR500-10?
The specified excitation wavelength is 535 nm. A 488 nm light source may produce weaker excitation and should be tested with the actual instrument.
How should CFR500-10 be dispersed?
Use gentle inversion, controlled vortexing or compatible bath sonication while avoiding excessive foam and aggregation.
Can CFR500-10 be customized?
Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer and packaging can be customized.
Are samples and bulk quantities available?
Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.
Request a Sample or Quotation
Contact SHBC for CFR500-10 samples, technical specifications, coupling support and bulk pricing.
Please provide:
Target application
Target biomarker
Assay format
Required quantity
Preferred packaging
Estimated annual demand
Product: 500nm Carboxyl Red-Fluo Microspheres
Catalog Number: CFR500-10
Particle Size: 500 nm
Solid Content: 1%
Excitation/Emission: 535/610 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.