Product Overview
CFR500-10 Carboxyl Red Fluorescent Microspheres are premium fluorescent polystyrene nanoparticles with reactive surface carboxyl groups for biomolecule conjugation and fluorescence-based detection. They combine bright red fluorescence, narrow particle size distribution and excellent colloidal stability to deliver reliable performance in diagnostic assays and biomedical research.
Supplied as a 1% aqueous suspension, CFR500-10 provides consistent fluorescence intensity and excellent dispersion stability. The surface carboxyl groups can be activated using EDC/NHS coupling chemistry, allowing stable covalent immobilization of antibodies, proteins, peptides and other amine-containing biomolecules for a wide variety of fluorescence applications.
Key Features
Uniform 500 nm particle size
Bright red fluorescence
Surface carboxyl functional groups
High fluorescence intensity
Excellent particle size uniformity
Stable aqueous suspension
Compatible with EDC/NHS coupling
High biomolecule conjugation efficiency
Low nonspecific adsorption
Excellent batch-to-batch consistency
Technical Specifications
Parameter | Specification |
|---|
Product Code | CFR500-10 |
Material | Fluorescent Polystyrene Microspheres |
Particle Size | 500 nm |
Surface | Carboxyl (-COOH) |
Solid Content | 1% Suspension |
Excitation (Ex) | 530 nm |
Emission (Em) | 580 nm |
Appearance | Red Fluorescent Suspension |
Storage | 2–8°C |
Packaging | 10 mL / 20 mL / 100 mL |
Applications
Lateral Flow Assays (LFA)
Fluorescence Immunoassays
Chemiluminescence Immunoassays (CLIA)
Antibody Conjugation
Protein Labeling
Cell Labeling
Biosensors
Molecular Diagnostics
Fluorescence Calibration
Life Science Research
Product Advantages
CFR500-10 combines intense red fluorescence with a highly reactive carboxyl-functionalized surface, enabling efficient biomolecule conjugation and highly sensitive fluorescence detection. The 500 nm particle size offers increased fluorescence brightness, higher biomolecule loading capacity and excellent suspension stability, making it particularly suitable for fluorescence immunoassays, lateral flow assays, biosensors, cell labeling and in vitro diagnostic reagent development.
Storage and Handling
Store at 2–8°C.
Do not freeze.
Protect from direct light.
Keep the container tightly sealed.
Gently invert before use.
Avoid repeated freeze-thaw cycles.
Prevent microbial contamination during handling.
Customization Service
SANYU GROUP provides customized fluorescent microspheres including:
Customized particle sizes
Customized fluorescence intensity
Customized excitation and emission wavelengths
Surface functionalization
Customized solid content
OEM manufacturing
Bulk production
Custom packaging
Frequently Asked Questions
What are Carboxyl Red Fluorescent Microspheres?
Carboxyl Red Fluorescent Microspheres are fluorescent polystyrene nanoparticles with reactive surface carboxyl groups designed for fluorescence detection, biomolecule conjugation and diagnostic assay development.
What are the excitation and emission wavelengths of CFR500-10?
CFR500-10 has an excitation wavelength of 530 nm and an emission wavelength of 580 nm, providing bright and stable red fluorescence for fluorescence-based analytical systems.
What applications is CFR500-10 suitable for?
CFR500-10 is widely used for lateral flow assays, fluorescence immunoassays, antibody conjugation, protein labeling, cell labeling, biosensors, molecular diagnostics, fluorescence calibration and life science research.
Can antibodies be conjugated to CFR500-10?
Yes. The surface carboxyl groups can be activated using EDC/NHS chemistry to covalently conjugate antibodies, proteins, peptides and other amine-containing biomolecules with high stability.
How should CFR500-10 be stored?
Store at 2–8°C, protect the suspension from light, avoid freezing, and gently invert before use to maintain optimal particle dispersion and fluorescence performance.
