50nm Carboxyl Green-Fluo Microspheres CFG050-10 0.5%

50nm Carboxyl Green-Fluo Microspheres CFG050-10

SHBC CFG050-10 are 50 nm carboxyl-functionalized green fluorescent microspheres developed for fluorescent immunoassays, lateral flow tests, biosensors and biomolecule labeling.

The particles feature excitation at 470/488 nm, emission at 525 nm and 0.5% solid content. Surface carboxyl groups support covalent coupling with antibodies, antigens, proteins and peptides.

Product Specifications

Batch-specific particle size, size distribution, fluorescence intensity and carboxyl content are available in the Certificate of Analysis.

Green Fluorescence at 470/488–525nm

CFG050-10 can be excited using approximately 470 nm or 488 nm light and emits green fluorescence near 525 nm.

The optical profile is suitable for compatible fluorescence readers, imaging systems and green-channel detection platforms.

Key Advantages

Uniform 50nm Particle Size

Controlled particle sizing supports consistent conjugation, dispersion and assay performance.

Bright Green Fluorescence

The 525 nm emission provides a clear signal for qualitative and quantitative fluorescence detection.

Reactive Carboxyl Surface

Surface COOH groups support covalent coupling of antibodies, antigens, proteins and other amino-containing molecules.

High Total Surface Area

The nanoscale particles provide a large total surface area for biomolecule immobilization.

Suitable for Membrane Assays

The 50 nm size can be evaluated in porous membranes and fluorescent lateral flow systems.

Custom and Bulk Supply

SHBC supports samples, customized specifications, pilot production and industrial batch supply.

Main Applications

Fluorescent Lateral Flow Assays

CFG050-10 can be evaluated as a fluorescent reporter nanoparticle in qualitative and quantitative lateral flow tests.

Fluorescence Immunoassays

Suitable for sandwich, competitive and indirect fluorescent immunoassay formats.

Antibody and Antigen Conjugation

Surface carboxyl groups allow covalent immobilization of antibodies, antigens and recombinant proteins.

Biosensor Development

Can be used as a fluorescent signal carrier in optical biosensors and surface-based detection systems.

Protein and Peptide Labeling

Suitable for coupling proteins, peptides, enzymes and other amino-containing biomolecules.

Nanoparticle Tracing

Can be evaluated in membrane transport, filtration, fluid-flow and particle-distribution research.

Diagnostic Reagent Development

Suitable for medical, veterinary, food-safety and environmental assay development.

Product Benefits

CFG050-10 provides:

• Green fluorescence near 525 nm

• Compatibility with 488 nm excitation

• Reactive COOH coupling sites

• High total coupling surface

• Nanoscale particle mobility

• Flexible biomolecule modification

• Sample and bulk supply

• Custom fluorescence options

Biomolecule Coupling

Surface carboxyl groups can be activated using EDC/NHS chemistry and coupled with primary amine groups on antibodies or proteins.

A general workflow includes:

1. Disperse and wash the microspheres.

2. Activate the carboxyl groups with EDC and NHS.

3. Add the antibody or target protein.

4. Block remaining reactive sites.

5. Purify and resuspend the conjugate.

6. Evaluate coupling and fluorescence performance.

Activation pH, reagent ratio, protein concentration and reaction time should be optimized for each biomolecule.

LFIA Development Considerations

For lateral flow applications, evaluate:

• Antibody-to-particle ratio

• Particle concentration

• Fluorescence intensity

• Nitrocellulose membrane type

• Conjugate pad release

• Running buffer composition

• Surfactant concentration

• Test-line capture density

• Background fluorescence

• Reader filter settings

The final formulation should provide both stable membrane migration and sufficient test-line signal.

Dispersion and Handling

Before use:

• Mix gently before sampling

• Use controlled bath sonication when required

• Avoid excessive bubbles and foam

• Prevent the particles from drying

• Protect the suspension from strong light

• Avoid repeated freeze-thaw cycles

• Confirm buffer compatibility

• Use clean laboratory equipment

High salt concentrations, unsuitable solvents and incorrect pH may reduce dispersion stability.

Purification Options

Because 50 nm particles may be difficult to collect using standard low-speed centrifugation, suitable purification methods may include:

• Ultracentrifugation

• Ultrafiltration

• Tangential flow filtration

• Size-exclusion chromatography

• Dialysis

Select the method according to sample volume, buffer composition and required recovery.

Assay Optimization

Important development parameters include:

• Carboxyl group density

• Ligand loading

• Coupling efficiency

• Blocking reagent

• Conjugate storage buffer

• Particle aggregation

• Membrane migration

• Fluorescence background

• Reader sensitivity

• Batch consistency

Optimization is recommended before production scale-up.

Application Limitations

Individual 50 nm particles may be below the direct detection limit of conventional flow cytometers.

Their signal is generally measured as an accumulated particle population, assay line or conjugated nanoparticle complex.

Instrument filters should be compatible with excitation at 470/488 nm and emission near 525 nm.

Custom Carboxyl Green-Fluo Microspheres

SHBC can customize fluorescent microspheres according to assay requirements.

Customization options may include:

• Custom particle size

• Custom excitation and emission wavelength

• Different fluorescence colors

• Carboxyl group density

• Fluorescence intensity

• Solid content

• Dispersion buffer

• Surface modification

• Custom packaging

• OEM and ODM production

Please provide the application, target biomolecule, required wavelength and order quantity when requesting customization.

Quality Control and Documents

Available product documents may include:

• Certificate of Analysis

• Technical Data Sheet

• Safety Data Sheet

• Particle size report

• Particle size distribution data

• Fluorescence spectrum

• Carboxyl content data

• Dispersion stability data

• Batch inspection report

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies fluorescent microspheres, colored latex particles, magnetic beads and functional microsphere materials.

SHBC provides:

• Controlled nanoparticle manufacturing

• Stable particle size distribution

• Multiple fluorescence wavelengths

• Functional surface modification

• Batch-to-batch consistency

• Sample evaluation support

• Pilot and bulk production

• OEM and ODM services

Frequently Asked Questions

What is CFG050-10?

CFG050-10 is a 50 nm carboxyl-functionalized green fluorescent microsphere suspension with 0.5% solid content.

What are the excitation and emission wavelengths?

The excitation wavelengths are 470/488 nm, and the emission wavelength is 525 nm.

What is CFG050-10 mainly used for?

It can be evaluated for fluorescent immunoassays, lateral flow tests, biosensors, biomolecule labeling and nanoparticle tracing.

Can antibodies be coupled to CFG050-10?

Yes. Antibodies and proteins containing primary amine groups can be covalently coupled to the carboxyl surface.

Which coupling method is recommended?

EDC/NHS chemistry is commonly used to activate carboxyl groups for protein coupling.

Can CFG050-10 be excited with a 488nm laser?

Yes. The specified excitation range includes 488 nm, with green emission near 525 nm.

Is CFG050-10 suitable for lateral flow assays?

Yes. Membrane type, particle concentration, conjugate release and reader settings should be optimized.

How does 50nm compare with 30nm particles?

The 50 nm particles may provide more fluorescence per particle, while 30 nm particles may offer faster diffusion. Actual assay performance should be compared experimentally.

Can the particles be collected by standard centrifugation?

Standard low-speed centrifugation is generally unsuitable. Ultracentrifugation, ultrafiltration, dialysis or chromatography may be required.

Can CFG050-10 be customized?

Yes. Particle size, fluorescence wavelength, carboxyl density, solid content, buffer and packaging can be customized.

Are samples and bulk quantities available?

Yes. SHBC supports laboratory samples, pilot orders and industrial batch production.

Request a Sample or Quotation

Contact SHBC for CFG050-10 samples, technical specifications, coupling support and bulk pricing.

Please provide:

• Target application

• Target biomolecule

• Assay format

• Detection instrument

• Required quantity

• Preferred packaging

• Estimated annual demand

Product: 50nm Carboxyl Green-Fluo Microspheres
Catalog Number: CFG050-10
Particle Size: 50 nm
Solid Content: 0.5%
Excitation/Emission: 470/488–525 nm
Surface Group: Carboxyl, COOH
Brand: SHBC
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.