Products
SHBC provides colored microspheres, fluorescent microspheres, magnetic beads, silica microspheres, chromatography packing microspheres and biological reagents for diagnostic assay development, nucleic acid extraction, protein purification and separation applications.
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PS/DVB5UM-300A
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SHBC
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5µm
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1g 10g 50g 100g 1000g
5µm Porous PS/DVB Microspheres with 300Å Pores
SHBC PS/DVB5UM-300A is a porous, crosslinked polystyrene-divinylbenzene microsphere with a nominal particle size of 5µm and a pore size of 300Å.
The rigid polymer matrix, hydrophobic surface and wide-pore structure make it suitable for chromatography packing, protein and peptide separation, oligonucleotide analysis and polymer separation research.
Product Overview
PS/DVB5UM-300A consists of spherical polystyrene particles crosslinked with divinylbenzene.
The DVB crosslinking improves particle rigidity and structural stability. The 5µm particle size supports efficient chromatographic separation, while the 300Å pore structure provides internal surface access for larger biomolecules.
The particles can be supplied as bulk chromatography media for column packing, method development and industrial separation research.
Key Features and Benefits
Nominal particle size of 5µm
Controlled 300Å pore size
Porous PS/DVB polymer matrix
Crosslinked spherical structure
Hydrophobic stationary-phase surface
High internal surface accessibility
Good mechanical stability
Suitable for chromatography packing
Suitable for proteins and peptides
Compatible with surface modification
Laboratory and bulk supply available
Custom particle and pore sizes supported
Product Specifications
Parameter | Specification |
|---|---|
Product Name | Porous Crosslinked PS/DVB Microspheres |
Catalog Number | PS/DVB5UM-300A |
Brand | SHBC |
Particle Size | 5µm |
Pore Size | 300Å |
Matrix | Polystyrene/Divinylbenzene |
Particle Structure | Porous and crosslinked |
Surface Property | Hydrophobic |
Product Form | Bulk microsphere packing media |
Manufacturer | Shanghai SanYu Biotechnology Co., Ltd. |
Intended Use | Research and industrial chromatography |
Additional parameters such as particle-size distribution, pore volume, specific surface area, crosslinking degree and pressure resistance are available according to the product specification.
How the Porous PS/DVB Matrix Works
The polystyrene backbone provides a hydrophobic surface that can interact with nonpolar regions of analytes.
Divinylbenzene creates a crosslinked network that strengthens the particles and helps maintain the porous structure during chromatography operation.
The internal 300Å pores increase the accessible surface area and allow larger analytes to enter the particle structure for chromatographic interaction.
Recommended Applications
Protein Separation
The 300Å pore structure can be evaluated for reversed-phase separation of intact proteins, protein subunits and larger biomolecules.
Peptide Purification
Suitable for analytical and preparative peptide separation, impurity removal and purity improvement.
Oligonucleotide Analysis
Can be used for chromatographic method development involving DNA or RNA oligonucleotides.
Monoclonal Antibody Research
Suitable for research involving intact antibodies, antibody subunits, fragments and related biomolecules.
Antibiotic Separation
Can be evaluated for the analysis and purification of hydrophobic antibiotics and related compounds.
Pharmaceutical Analysis
Suitable for chromatographic separation of small-molecule pharmaceuticals and process-related impurities.
Polymer Separation
The porous PS/DVB matrix can be evaluated for gel permeation or size exclusion chromatography of synthetic polymers in compatible mobile phases.
Chromatography Media Development
Can be used as a base material for developing custom stationary phases and functionalized separation media.
Advantages of 300Å Pore Size
Access for Larger Biomolecules
The wide-pore structure provides better internal access for proteins, peptides, oligonucleotides and other relatively large analytes.
Increased Available Surface
The porous structure provides more chromatographically active surface than nonporous particles.
Suitable for Biopolymer Analysis
The 300Å pore size is suitable for method development involving biomolecules that may have limited access to smaller pores.
Balanced Resolution and Mass Transfer
The combination of 5µm particles and wide pores supports efficient chromatographic interaction and separation performance.
Advantages of the PS/DVB Matrix
Crosslinked Particle Rigidity
DVB crosslinking improves the mechanical integrity of the polymer particles during column packing and operation.
Hydrophobic Retention
The polystyrene matrix provides inherent hydrophobic interaction without requiring a conventional bonded C18 layer.
No Silanol Surface
Unlike silica-based materials, PS/DVB particles do not contain residual silanol groups that may cause unwanted interactions with basic analytes.
Flexible Method Development
The polymer matrix can be evaluated with different aqueous and organic mobile-phase systems according to the validated method.
Surface Modification Potential
The particles can be modified with alternative functional groups or coatings for specialized chromatographic applications.
Chromatography Packing Applications
PS/DVB5UM-300A can be used for packing:
Analytical HPLC columns
Preparative chromatography columns
Protein purification columns
Peptide separation columns
Oligonucleotide analysis columns
Polymer SEC or GPC columns
Custom research columns
Process-development columns
Column dimensions, packing pressure and slurry conditions should be optimized for the chromatography hardware and intended application.
General Column Packing Procedure
Select a compatible slurry solvent.
Fully disperse the microspheres before packing.
Prepare a uniform slurry without visible agglomerates.
Transfer the slurry into the column or packing reservoir.
Pack under controlled flow or pressure.
Avoid excessive pressure that may compress the packed bed.
Flush the column with the selected mobile phase.
Continue equilibration until a stable baseline is obtained.
Test column efficiency using a suitable standard.
Confirm pressure, peak symmetry and retention reproducibility.
Packing conditions should be validated for each column diameter, bed length and chromatography system.
Handling Recommendations
Avoid inhalation of dry particles.
Use suitable personal protective equipment.
Prevent contamination during handling.
Fully disperse the particles before slurry preparation.
Avoid prolonged exposure to unvalidated solvents.
Do not exceed the validated packing pressure.
Store in a tightly sealed container.
Follow the product safety documentation.
Why Choose SHBC PS/DVB Microspheres
Controlled Particle Manufacturing
SHBC uses controlled polymerization and particle-classification processes to support consistent 5µm particle size.
Controlled Porous Structure
The 300Å pore structure is designed for chromatography applications involving proteins, peptides and other biomolecules.
Reliable Batch Consistency
Particle size, pore structure and polymer properties are controlled to support reproducible chromatography performance.
Flexible Product Development
Particle size, pore size, crosslinking degree and surface chemistry can be customized.
Bulk Manufacturing Capability
SHBC supports laboratory evaluation, pilot-scale column development and industrial batch supply.
Technical Support
Support is available for media selection, slurry preparation, column packing and application development.
Custom and Bulk Supply
Shanghai SanYu Biotechnology Co., Ltd. supplies porous PS/DVB microspheres to universities, pharmaceutical companies, chromatography manufacturers and purification-process developers.
Available customization includes:
Particle size
Pore size
Pore volume
Specific surface area
Crosslinking degree
Surface functionality
Product form
Packaging quantity
OEM and ODM manufacturing
Batch quality documentation
Please provide the target analyte, chromatography mode, column dimensions, required pore size and estimated order quantity when requesting a quotation.
Frequently Asked Questions
What is PS/DVB5UM-300A?
PS/DVB5UM-300A is a porous, crosslinked polystyrene-divinylbenzene microsphere with a 5µm particle size and 300Å pore size.
What does 300Å mean?
It refers to the nominal pore diameter inside the porous polymer microspheres.
What is the purpose of the pores?
The pores provide internal surface area and allow analytes to enter the particle structure for chromatographic interaction.
What molecules are suitable for 300Å pores?
The material can be evaluated for proteins, peptides, antibody fragments, oligonucleotides and other biomolecules.
Is the surface hydrophobic?
Yes. The standard PS/DVB matrix has an inherently hydrophobic polymer surface.
Can it be used for reversed-phase chromatography?
Yes. The hydrophobic PS/DVB surface can be used as a reversed-phase stationary-phase base material.
Can it be used for SEC or GPC?
It can be evaluated for size exclusion or gel permeation chromatography when the pore structure and mobile phase match the target polymer range.
Is this a finished HPLC column?
No. PS/DVB5UM-300A is supplied as bulk porous microsphere packing media unless a prepacked column is specifically ordered.
Can SHBC customize the pore size?
Yes. Particle size, pore size, crosslinking degree and surface chemistry can be customized.
Is bulk production available?
Yes. SHBC supports samples, pilot quantities and bulk manufacturing.
Request a Sample or Quotation
Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical documentation, customized specifications or bulk pricing.
Product: 5µm Porous Crosslinked PS/DVB Microspheres
Catalog Number: PS/DVB5UM-300A
Brand: SHBC
Particle Size: 5µm
Pore Size: 300Å
Matrix: Polystyrene/Divinylbenzene
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.
For research and industrial chromatography use only.