5um Epoxy Silicate Microspheres ESi5UM-10 5%

5µm Epoxy Silicate Microspheres ESi5UM-10

SHBC ESi5UM-10 is a 5µm epoxy-functionalized silicate microsphere suspension with 5% solids. The microspheres provide a reactive solid surface for the covalent immobilization of proteins, antibodies, enzymes, peptides and amino-modified biomolecules.

Also known as epoxy silica microspheres, ESi5UM-10 is suitable for bioassay development, biomolecule coupling, affinity separation and functional material research.

Product Overview

The 5µm particle size provides convenient separation, washing and solid-phase handling during laboratory and process development.

Reactive epoxy groups are introduced onto the microsphere surface to support direct coupling with suitable functional groups on biomolecules. The silicate-based particle matrix provides a stable carrier for surface modification and immobilization research.

Key Features and Benefits

• Uniform 5µm particle size

• Reactive epoxy-functionalized surface

• 5% solid concentration

• Suitable for covalent biomolecule immobilization

• Convenient particle collection and washing

• Stable silicate-based microsphere matrix

• Suitable for research and process development

• Custom concentration and packaging available

• Reliable batch and bulk supply

Product Specifications

How Epoxy Silicate Microspheres Work

Epoxy groups can react with nucleophilic groups under suitable coupling conditions. Common targets include primary amino groups found in proteins, antibodies, peptides and amino-modified probes.

This surface chemistry enables biomolecules to be covalently immobilized on the microspheres. Compared with physical adsorption, covalent attachment can provide more stable retention during washing, separation and downstream processing.

A separate EDC/NHS activation step is generally not required for direct epoxy coupling.

Recommended Applications

Antibody and Protein Immobilization

ESi5UM-10 can be used as a solid carrier for immobilizing antibodies, antigens, recombinant proteins and peptides.

Enzyme Immobilization

The epoxy surface supports enzyme coupling for biocatalysis research, enzyme carrier development and reusable reaction systems.

Immunoassay Development

Suitable for developing bead-based immunoassays, antigen-antibody interaction studies and diagnostic reagent research.

Nucleic Acid Probe Coupling

Can be used with amino-modified DNA, RNA and oligonucleotide probes for molecular assay development.

Affinity Separation

Suitable for preparing ligand-functionalized particles used in biomolecule capture, affinity purification and separation research.

Surface Modification Research

The epoxy surface can be further modified with amino-containing ligands, polymers and functional molecules.

General Coupling Procedure

1. Mix the microsphere suspension thoroughly before use.

2. Transfer the required amount of ESi5UM-10 into a reaction tube.

3. Wash the microspheres with a suitable coupling buffer.

4. Add the antibody, protein, enzyme or amino-modified molecule.

5. Incubate under optimized coupling conditions.

6. Block the remaining reactive sites when necessary.

7. Wash away unbound biomolecules.

8. Resuspend the coupled microspheres in a suitable storage buffer.

Coupling time, pH, temperature and biomolecule concentration should be optimized for the specific application.

Advantages of 5µm Particle Size

The 5µm diameter provides a practical balance between surface functionality and handling performance.

Compared with smaller microspheres, 5µm particles are easier to collect by centrifugation, wash and resuspend. They are suitable for solid-phase reactions, bead-based assays and separation processes that require convenient particle recovery.

Why Choose SHBC

Controlled Particle Manufacturing

SHBC applies controlled microsphere manufacturing processes to support consistent particle size and surface functionality.

Reactive Epoxy Surface

The epoxy-functionalized surface provides a convenient route for covalent coupling of amino-containing biomolecules.

Flexible Customization

Particle size, solid concentration, surface group, packaging volume and buffer system can be customized according to project requirements.

Scale-Up Support

SHBC supports laboratory evaluation, process development, pilot orders and bulk production.

Technical Service

Technical support is available for particle selection, coupling condition development and application evaluation.

Custom and Bulk Supply

Shanghai SanYu Biotechnology Co., Ltd. supplies epoxy-functionalized silicate microspheres to universities, research institutes, biotechnology companies and diagnostic reagent manufacturers.

Available services include:

• Custom particle size

• Custom solid concentration

• Alternative surface functional groups

• Custom dispersion medium

• Sample evaluation

• Biomolecule coupling support

• OEM and ODM manufacturing

• Pilot-scale and bulk production

• Custom packaging

• Batch documentation

Please provide the required particle size, concentration, application and estimated order quantity when requesting a quotation.

Frequently Asked Questions

What is ESi5UM-10?

ESi5UM-10 is a 5µm epoxy-functionalized silicate microsphere suspension supplied at 5% solids.

What can be coupled to the microspheres?

Typical coupling targets include antibodies, proteins, peptides, enzymes, amino-modified oligonucleotides and amino-containing ligands.

Does epoxy coupling require EDC/NHS activation?

A separate EDC/NHS activation step is generally not required because the surface already contains reactive epoxy groups.

Why select 5µm microspheres?

The 5µm particle size offers convenient collection, washing and resuspension for solid-phase coupling and separation applications.

Can the product be used for immunoassay development?

Yes. ESi5UM-10 can be evaluated as a functional carrier for antibody immobilization, bead-based immunoassays and diagnostic reagent research.

Can SHBC customize the concentration?

Yes. Solid content, particle size, surface chemistry, dispersion medium and packaging can be customized.

Is bulk production available?

Yes. SHBC supports samples, pilot-scale orders and bulk manufacturing for research and industrial customers.

Storage and Handling

Store the product according to the conditions stated in the product documentation.

Do not freeze unless specifically recommended. Mix gently before use and avoid contamination during sampling. Appropriate washing and dispersion conditions should be selected according to the final application.

Request a Sample or Quotation

Contact Shanghai SanYu Biotechnology Co., Ltd. for samples, technical information, customization and bulk pricing.

Product: 5µm Epoxy Silicate Microspheres
Catalog Number: ESi5UM-10
Brand: SHBC
Particle Size: 5µm
Solid Content: 5%
Surface Group: Epoxy
Manufacturer: Shanghai SanYu Biotechnology Co., Ltd.

For research and industrial raw material use only.