5um Nickel Magnetic Beads MNi5UM-10 5%

5um Nickel Magnetic Beads MNi5UM-10

SHBC MNi5UM-10 Nickel Magnetic Beads are 5 μm magnetic microspheres with a nickel-affinity surface and 5% solid content.

They are designed for magnetic capture, separation and purification of His-tagged proteins in research and process-development workflows.

Product Overview

MNi5UM-10 combines a 5 μm particle size with nickel affinity and magnetic separation.

The beads provide a convenient solid phase for capturing polyhistidine-tagged proteins from cell lysates, clarified samples and protein solutions.

The concentrated 5% suspension supports flexible dilution, manual processing, automation development and bulk applications.

Technical Specifications

The exact chelating ligand, binding capacity, magnetic response and buffer composition are batch- or specification-dependent. Refer to the applicable TDS and COA.

Key Advantages

• 5 μm magnetic particle size

• Concentrated 5% solids

• Nickel-affinity surface

• Convenient magnetic separation

• No centrifugation required for bead collection

• Suitable for His-tagged protein capture

• Compatible with small-scale purification workflows

• Suitable for manual and automated process development

• Flexible packaging volumes

• OEM and bulk production available

How Nickel Magnetic Beads Work

Nickel affinity is commonly used in immobilized metal affinity chromatography, also known as IMAC.

Histidine residues in a His-tag interact with immobilized nickel ions on the bead surface. After binding, a magnet collects the beads while unbound sample components are removed.

The captured target protein can then be washed and eluted under an application-specific protocol.

Applications of 5um Nickel Magnetic Beads

His-Tagged Protein Purification

MNi5UM-10 is designed for the capture and purification of recombinant proteins containing polyhistidine tags.

Recombinant Protein Screening

The beads can support rapid screening of His-tagged protein expression from multiple samples.

Protein Pull-Down Assays

His-tagged bait proteins can be immobilized on the beads for protein-interaction and pull-down research.

His-Tagged Protein Immobilization

MNi5UM-10 may be used to immobilize His-tagged proteins for downstream binding, screening and analytical studies.

Magnetic Protein Separation

The magnetic core enables convenient separation using a compatible magnetic rack or automated magnetic platform.

High-Throughput Process Development

The bead suspension can be evaluated in tubes, multiwell plates and automated liquid-handling workflows.

Native and Denaturing Purification Research

Nickel-affinity purification can be developed under native or denaturing conditions, subject to the target protein and selected buffer system.

Typical Purification Workflow

1. Resuspend the Beads

Mix the bead suspension thoroughly before sampling to obtain a uniform particle concentration.

2. Wash and Equilibrate

Collect the beads with a magnet, remove the storage solution and equilibrate them with a suitable binding buffer.

3. Add the Protein Sample

Add clarified lysate or protein solution and mix gently to allow His-tagged proteins to interact with the nickel surface.

4. Perform Magnetic Separation

Place the sample on a compatible magnet and remove the unbound fraction after the beads are collected.

5. Wash the Beads

Wash the beads to reduce nonspecific proteins and other sample components.

6. Elute the Target Protein

Elute the captured His-tagged protein using a validated imidazole, pH-based or other compatible elution method.

Protocol conditions should be optimized for the target protein and sample matrix.

Benefits of 5um Particle Size

The 5 μm particle size offers a practical balance between accessible surface area, suspension handling and magnetic collection.

It is suitable for:

• Tube-based protein purification

• Magnetic-rack separation

• Multiwell plate workflows

• Automated process development

• Repeated washing and elution

• Pilot-scale assay optimization

Benefits of 5% Solid Content

MNi5UM-10 is supplied at 5% solids to provide a concentrated magnetic bead suspension.

Users can dilute the beads according to sample volume, target concentration and required binding performance.

The concentrated format also supports repeated experiments, pilot projects and bulk manufacturing requirements.

Buffer Compatibility Considerations

Nickel-affinity performance depends on buffer composition, pH, salt concentration and competing reagents.

Users should evaluate:

• Binding-buffer pH

• Imidazole concentration

• Detergent concentration

• Reducing agents

• Chelating agents

• Protein denaturants

• Sample viscosity

Strong chelating agents may remove nickel ions from the affinity surface. Compatibility should be confirmed before use.

Factors Affecting Purification Performance

Purification efficiency may be influenced by:

• Accessibility of the His tag

• Number and position of histidine residues

• Target protein concentration

• Sample pH

• Incubation time

• Mixing efficiency

• Wash conditions

• Elution conditions

• Presence of competing proteins

• Bead-to-sample ratio

Small-scale optimization is recommended before batch processing.

Quality and Batch Documentation

Available documentation may include:

• Product code

• Batch number

• Nominal particle size

• Solid content

• Product appearance

• Surface type

• Certificate of Analysis

• Safety Data Sheet

• Technical Data Sheet

Binding capacity and magnetic response should be quoted only according to the applicable product specification or batch documentation.

Why Choose SHBC?

Shanghai SanYu Biotechnology Co., Ltd. supplies magnetic beads and functional microspheres for protein purification, biomolecule separation and assay development.

SHBC supply advantages include:

• Multiple magnetic bead sizes

• Nickel and other functional surfaces

• Laboratory sample supply

• Pilot-scale manufacturing

• Bulk production capability

• Customized solid content

• Flexible packaging volumes

• OEM and private-label services

• Technical product-selection support

Customized Nickel Magnetic Beads

Customization options may include:

• Particle diameter

• Particle-size distribution

• Solid content

• Nickel-affinity surface

• Chelating ligand

• Dispersion medium

• Binding-buffer system

• Packaging volume

• OEM labeling

• Bulk production

Storage and Handling

Store MNi5UM-10 according to the product label, TDS, COA and SDS.

Thoroughly resuspend the beads before sampling. Avoid allowing the magnetic beads to dry during washing and purification.

Use clean, low-binding laboratory tools and a compatible magnetic separator. Do not freeze unless freezing compatibility is confirmed in the product specification.

Frequently Asked Questions

What is MNi5UM-10?

MNi5UM-10 is a 5 μm nickel-affinity magnetic bead suspension with a solid content of 5%.

What are nickel magnetic beads used for?

They are mainly used for magnetic capture, separation, purification and immobilization of His-tagged proteins.

What does His-tag mean?

A His-tag is a sequence of histidine residues added to a recombinant protein to enable affinity capture using immobilized metal ions such as nickel.

What is IMAC?

IMAC means immobilized metal affinity chromatography. It uses immobilized metal ions to capture proteins containing accessible histidine residues.

Can MNi5UM-10 purify 6×His-tagged proteins?

The beads are designed for polyhistidine-tagged protein capture. Performance depends on tag accessibility, buffer conditions and the target protein.

Can the beads be used without centrifugation?

Yes. A compatible magnet can be used to collect the beads and remove liquid fractions.

Can the beads be used in automated systems?

MNi5UM-10 may be evaluated for automated magnetic-separation and multiwell-plate workflows.

Can the beads be used under denaturing conditions?

Nickel-affinity purification can be developed under selected denaturing conditions. Compatibility must be verified for the bead formulation and target protein.

Can EDTA be used with nickel magnetic beads?

Strong chelating agents such as EDTA may remove nickel ions and reduce binding performance. Use should be avoided unless compatibility is confirmed.

Can imidazole be used?

Imidazole is commonly evaluated at controlled concentrations during binding, washing and elution. The optimal concentration depends on the target and sample.

What is the binding capacity?

Binding capacity depends on the bead formulation, target protein and purification conditions. Refer to the applicable SHBC technical specification.

Are bulk quantities available?

Yes. SHBC supports laboratory samples, customized packaging, pilot projects and bulk production.

Request MNi5UM-10 Samples and Quotations

Contact Shanghai SanYu Biotechnology Co., Ltd. for MNi5UM-10 samples, technical specifications, application support, customized packaging and bulk quotations.

SHBC supplies nickel magnetic beads for recombinant protein purification, affinity capture, assay development and industrial research.

For Research Use Only.